Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
Context: Muntingia calabura L. (Muntingiaceae) exerts antioxidant and anti-inflammatory activities, thus, it might be a good hepatoprotective agent. Objective: This study investigates the effect of methanol extract of M. calabura leaves (MMCL) on hepatic antioxidant and anti-inflammatory activities...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Taylor and Francis
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/72035/ http://irep.iium.edu.my/72035/ http://irep.iium.edu.my/72035/ http://irep.iium.edu.my/72035/1/Pharmbiol_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates%20CCl4%20induced%20liver%20injury%20possible%20synergistic%20action%20of%20flavonoids%20and%20volatile%20bioactive.pdf http://irep.iium.edu.my/72035/7/72035_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates%20CCl4_scopus.pdf |
| Summary: | Context: Muntingia calabura L. (Muntingiaceae) exerts antioxidant and anti-inflammatory activities, thus, it might be a good hepatoprotective agent.
Objective: This study investigates the effect of methanol extract of M. calabura leaves (MMCL) on hepatic antioxidant and anti-inflammatory activities in CCl4-induced hepatotoxic rat.
Materials and methods: Sprague Dawley rats (n 1⁄4 6) were treated (p.o.) with 10% DMSO (Groups 1 and 2), 50mg/kg N-acetylcysteine (Group 3) or, 50, 250, or 500mg/kg MMCL (Groups 4–6) for 7 consecutive days followed by pretreatment (i.p.) with vehicle (Group 1) or 50% CCl4 in olive oil (v/v) (Groups 2–6) on day 7th. Plasma liver enzymes and hepatic antioxidant enzymes and pro-inflammatory cytokines concen- trations were measured while liver histopathology was examined.
Results: MMCL, at 500 mg/kg, significantly (p < 0.05) ameliorated CCl4-induced hepatotoxicity by decreas- ing the plasma level of alanine transaminase (429.1 versus 168.7U/L) and aspartate transaminase (513.8 versus 438.1 U/L) as well as the tissue level of nitric oxide (62.7 versus 24.1 nmol/g tissue). At 50, 250, or 500mg/kg, MMCL significantly (p<0.05) reduced the tumour necrosis factor a (87.8 versus 32.7pg/mg tissue), interleukin-1b (1474.4 versus 618.3pg/mg tissue), and interleukin-6 (136.7 versus 30.8pg/mg tis- sue) while increased the liver catalase (92.1 versus 114.4 U/g tissue) and superoxide dismutase (3.4 versus 5.5 U/g tissue). Additionally, qualitative phytochemicals analysis showed that MMCL contained gallic acid, ferulic acid, quercetin, and genistein.
Discussion and conclusions: MMCL ability to attenuate CCl4-induced hepatotoxicity could be helpful in the development of hepatoprotective agents with fewer side effects. |
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