Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells

Apart from the highly sought agarwood resin primarily for perfumery industry, agarwood or gaharu (Aquilaria spp.) in general has been one of basic components in traditional medicine including Ayurvedic, Traditional Chinese Medicine and other communities in the Asian region. While the resin is form...

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Main Authors:	Abbas, Phirdaous, Hashim, Yumi Zuhanis Has-Yun, Mohd. Salleh, Hamzah
Format:	Article
Language:	English English English
Published:	Marmara University Press 2019
Subjects:	Q Science (General) TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/72088/ http://irep.iium.edu.my/72088/ http://irep.iium.edu.my/72088/ http://irep.iium.edu.my/72088/1/72088_Uninfected%20agarwood%20branch.pdf http://irep.iium.edu.my/72088/2/72088_Uninfected%20agarwood%20branch_WOS.pdf http://irep.iium.edu.my/72088/13/72088_Uninfected%20agarwood%20branch%20extract%20possess%20cytotoxic%20and%20inhibitory%20effects_scopus.pdf

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recordtype	eprints
spelling	iium-720882019-08-05T03:02:34Z http://irep.iium.edu.my/72088/ Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah Q Science (General) TP248.13 Biotechnology Apart from the highly sought agarwood resin primarily for perfumery industry, agarwood or gaharu (Aquilaria spp.) in general has been one of basic components in traditional medicine including Ayurvedic, Traditional Chinese Medicine and other communities in the Asian region. While the resin is formed in infected trees, this present study reported the cytotoxicity and attachment inhibition effects of leaf and branch extracts from uninfected agarwood tree against breast cancer cells. Qualitative extraction screening process was first conducted to screen for suitable extraction solvents and parts of plant (leaf or branch). Then, the in vitro anti-cancer screening assays including cytotoxicity and attachment assays were conducted. Branch sample extracted using ethanol and distilled water resulted in higher yield and more potent cancer inhibiting effects as compared to other solvents. Crude extract obtained after drying process using ethanol as solvent yielded 9.47 % g/g (branch) and 13.2 % g/g (leaf); distilled water as solvent yielded 9.33 % g/g (branch) and 12.8 % g/g (leaf), respectively. However, branch extract exhibited more potent cancerinhibiting effects with IC50 of 23 µg/mL (ethanol) and 38 µg/mL (distilled water) as compared to leaf (no intersection points in the plot). To this end, it can be concluded that extracts from uninfected agarwood tree (Aquilaria subintegra) possesses cytotoxic and anti-attachment effects on MCF-7 breast cancer cells with ethanolic branch extract being the most promising. The screening and selection of extraction solvent and plant type are crucial steps towards cost-effective extraction and further bioprocessing of bioactive compounds from agarwood tree. Marmara University Press 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/72088/1/72088_Uninfected%20agarwood%20branch.pdf application/pdf en http://irep.iium.edu.my/72088/2/72088_Uninfected%20agarwood%20branch_WOS.pdf application/pdf en http://irep.iium.edu.my/72088/13/72088_Uninfected%20agarwood%20branch%20extract%20possess%20cytotoxic%20and%20inhibitory%20effects_scopus.pdf Abbas, Phirdaous and Hashim, Yumi Zuhanis Has-Yun and Mohd. Salleh, Hamzah (2019) Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells. Journal of Research in Pharmacy, 23 (1). pp. 120-129. ISSN 2630-6344 http://jrespharm.com/uploads/pdf/pdf_MPJ_637.pdf https://doi.org/10.12991/jrp.2018.116
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	Q Science (General) TP248.13 Biotechnology
spellingShingle	Q Science (General) TP248.13 Biotechnology Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells
description	Apart from the highly sought agarwood resin primarily for perfumery industry, agarwood or gaharu (Aquilaria spp.) in general has been one of basic components in traditional medicine including Ayurvedic, Traditional Chinese Medicine and other communities in the Asian region. While the resin is formed in infected trees, this present study reported the cytotoxicity and attachment inhibition effects of leaf and branch extracts from uninfected agarwood tree against breast cancer cells. Qualitative extraction screening process was first conducted to screen for suitable extraction solvents and parts of plant (leaf or branch). Then, the in vitro anti-cancer screening assays including cytotoxicity and attachment assays were conducted. Branch sample extracted using ethanol and distilled water resulted in higher yield and more potent cancer inhibiting effects as compared to other solvents. Crude extract obtained after drying process using ethanol as solvent yielded 9.47 % g/g (branch) and 13.2 % g/g (leaf); distilled water as solvent yielded 9.33 % g/g (branch) and 12.8 % g/g (leaf), respectively. However, branch extract exhibited more potent cancerinhibiting effects with IC50 of 23 µg/mL (ethanol) and 38 µg/mL (distilled water) as compared to leaf (no intersection points in the plot). To this end, it can be concluded that extracts from uninfected agarwood tree (Aquilaria subintegra) possesses cytotoxic and anti-attachment effects on MCF-7 breast cancer cells with ethanolic branch extract being the most promising. The screening and selection of extraction solvent and plant type are crucial steps towards cost-effective extraction and further bioprocessing of bioactive compounds from agarwood tree.
format	Article
author	Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah
author_facet	Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah
author_sort	Abbas, Phirdaous
title	Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells
title_short	Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells
title_full	Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells
title_fullStr	Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells
title_full_unstemmed	Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells
title_sort	uninfected agarwood branch extract possess cytotoxic and inhibitory effects on mcf-7 breast cancer cells
publisher	Marmara University Press
publishDate	2019
url	http://irep.iium.edu.my/72088/ http://irep.iium.edu.my/72088/ http://irep.iium.edu.my/72088/ http://irep.iium.edu.my/72088/1/72088_Uninfected%20agarwood%20branch.pdf http://irep.iium.edu.my/72088/2/72088_Uninfected%20agarwood%20branch_WOS.pdf http://irep.iium.edu.my/72088/13/72088_Uninfected%20agarwood%20branch%20extract%20possess%20cytotoxic%20and%20inhibitory%20effects_scopus.pdf
first_indexed	2023-09-18T21:42:12Z
last_indexed	2023-09-18T21:42:12Z
_version_	1777413214573690880

Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells

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