Antiproliferative activity of ionic liquid-graviola fruit extract against human breast cancer (MCF-7) cell lines using flow cytometry techniques

Ethnopharmacological relevance Medicinal plants have been used for ages by indigenous communities around the world to help humankind sustain its health. Graviola (Annona muricata), also called soursop, is a member of the Annonaceae family and is an evergreen plant that is generally distributed in t...

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Main Authors:	Daddiouaissa, Djabir, Amid, Azura, Kabbashi, Nassereldeen Ahmed, Ahmad Fuad, Fazia Adyani, Elnour, Ahmed, Epandy, Mohamad A.K.M S.
Format:	Article
Language:	English English English
Published:	Elsevier Ireland Ltd 2019
Subjects:	TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/72405/ http://irep.iium.edu.my/72405/ http://irep.iium.edu.my/72405/ http://irep.iium.edu.my/72405/1/72405_Antiproliferative%20activity%20of%20ionic.pdf http://irep.iium.edu.my/72405/2/72405_Antiproliferative%20activity%20of%20ionic_SCOPUS.pdf http://irep.iium.edu.my/72405/3/72405_Antiproliferative%20activity%20of%20ionic_WOS.pdf

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spelling	iium-724052019-07-12T01:15:09Z http://irep.iium.edu.my/72405/ Antiproliferative activity of ionic liquid-graviola fruit extract against human breast cancer (MCF-7) cell lines using flow cytometry techniques Daddiouaissa, Djabir Amid, Azura Kabbashi, Nassereldeen Ahmed Ahmad Fuad, Fazia Adyani Elnour, Ahmed Epandy, Mohamad A.K.M S. TP Chemical technology TP248.13 Biotechnology Ethnopharmacological relevance Medicinal plants have been used for ages by indigenous communities around the world to help humankind sustain its health. Graviola (Annona muricata), also called soursop, is a member of the Annonaceae family and is an evergreen plant that is generally distributed in tropical and subtropical areas of the world. Graviola tree has a long history of traditional use due to its therapeutic potential including anti-inflammatory, antimicrobial, antioxidant, insecticide and cytotoxic to tumor cells. Aim of the study This study aimed to investigate the in vitro antiproliferative effects and apoptotic events of the ionic liquid extract of Graviola fruit (IL-GFE) on MCF-7 breast cancer cells and their cytokinetics behaviour to observe their potential as a therapeutic alternative in cancer treatment. Materials and methods The cell viability assay of the extract was measured using tetrazolium bromide (MTT assay) to observe the effects of Graviola fruit extract. Then the cytokinetics behaviour of MCF-7 cells treated with IL-GFE is observed by plotting the growth curve of the cells. Additionally, the cell cycle distribution and apoptosis mechanism of IL-GFE action on MCF-7 cancer cells were observed by flow cytometry. Results IL-GFE exhibited anti-proliferative activity on MCF-7 with the IC50 value of 4.75 μg/mL, compared to Taxol with an IC50 value of 0.99 μg/mL. IL- GFE also reduced the number of cell generations from 3.71 to 1.67 generations compared to 2.18 generations when treated with Taxol. Furthermore, the anti-proliferative activities were verified when the growth rate was decreased dynamically from 0.0077 h to 1 to 0.0035 h-1. Observation of the IL-GFE-treated MCF-7 under microscope demonstrated detachment of cells and loss of density. The growth inhibition of the cells by extracts was associated with cell cycle arrest at the G0/G1 phase, and phosphatidylserine externalisation confirms the anti-proliferation through apoptosis. Conclusions ionic liquid Graviola fruit extract affect the cytokinetics behaviour of MCF-7 cells by reducing cell viability, induce apoptosis and cell cycle arrest at the G0/G1 phase. Elsevier Ireland Ltd 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/72405/1/72405_Antiproliferative%20activity%20of%20ionic.pdf application/pdf en http://irep.iium.edu.my/72405/2/72405_Antiproliferative%20activity%20of%20ionic_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/72405/3/72405_Antiproliferative%20activity%20of%20ionic_WOS.pdf Daddiouaissa, Djabir and Amid, Azura and Kabbashi, Nassereldeen Ahmed and Ahmad Fuad, Fazia Adyani and Elnour, Ahmed and Epandy, Mohamad A.K.M S. (2019) Antiproliferative activity of ionic liquid-graviola fruit extract against human breast cancer (MCF-7) cell lines using flow cytometry techniques. Journal of Ethnopharmacology, 236. pp. 466-473. ISSN 0378-8741 https://www.sciencedirect.com/science/article/pii/S0378874118347901 10.1016/j.jep.2019.03.003
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TP Chemical technology TP248.13 Biotechnology
spellingShingle	TP Chemical technology TP248.13 Biotechnology Daddiouaissa, Djabir Amid, Azura Kabbashi, Nassereldeen Ahmed Ahmad Fuad, Fazia Adyani Elnour, Ahmed Epandy, Mohamad A.K.M S. Antiproliferative activity of ionic liquid-graviola fruit extract against human breast cancer (MCF-7) cell lines using flow cytometry techniques
description	Ethnopharmacological relevance Medicinal plants have been used for ages by indigenous communities around the world to help humankind sustain its health. Graviola (Annona muricata), also called soursop, is a member of the Annonaceae family and is an evergreen plant that is generally distributed in tropical and subtropical areas of the world. Graviola tree has a long history of traditional use due to its therapeutic potential including anti-inflammatory, antimicrobial, antioxidant, insecticide and cytotoxic to tumor cells. Aim of the study This study aimed to investigate the in vitro antiproliferative effects and apoptotic events of the ionic liquid extract of Graviola fruit (IL-GFE) on MCF-7 breast cancer cells and their cytokinetics behaviour to observe their potential as a therapeutic alternative in cancer treatment. Materials and methods The cell viability assay of the extract was measured using tetrazolium bromide (MTT assay) to observe the effects of Graviola fruit extract. Then the cytokinetics behaviour of MCF-7 cells treated with IL-GFE is observed by plotting the growth curve of the cells. Additionally, the cell cycle distribution and apoptosis mechanism of IL-GFE action on MCF-7 cancer cells were observed by flow cytometry. Results IL-GFE exhibited anti-proliferative activity on MCF-7 with the IC50 value of 4.75 μg/mL, compared to Taxol with an IC50 value of 0.99 μg/mL. IL- GFE also reduced the number of cell generations from 3.71 to 1.67 generations compared to 2.18 generations when treated with Taxol. Furthermore, the anti-proliferative activities were verified when the growth rate was decreased dynamically from 0.0077 h to 1 to 0.0035 h-1. Observation of the IL-GFE-treated MCF-7 under microscope demonstrated detachment of cells and loss of density. The growth inhibition of the cells by extracts was associated with cell cycle arrest at the G0/G1 phase, and phosphatidylserine externalisation confirms the anti-proliferation through apoptosis. Conclusions ionic liquid Graviola fruit extract affect the cytokinetics behaviour of MCF-7 cells by reducing cell viability, induce apoptosis and cell cycle arrest at the G0/G1 phase.
format	Article
author	Daddiouaissa, Djabir Amid, Azura Kabbashi, Nassereldeen Ahmed Ahmad Fuad, Fazia Adyani Elnour, Ahmed Epandy, Mohamad A.K.M S.
author_facet	Daddiouaissa, Djabir Amid, Azura Kabbashi, Nassereldeen Ahmed Ahmad Fuad, Fazia Adyani Elnour, Ahmed Epandy, Mohamad A.K.M S.
author_sort	Daddiouaissa, Djabir
title	Antiproliferative activity of ionic liquid-graviola fruit extract against human breast cancer (MCF-7) cell lines using flow cytometry techniques
title_short	Antiproliferative activity of ionic liquid-graviola fruit extract against human breast cancer (MCF-7) cell lines using flow cytometry techniques
title_full	Antiproliferative activity of ionic liquid-graviola fruit extract against human breast cancer (MCF-7) cell lines using flow cytometry techniques
title_fullStr	Antiproliferative activity of ionic liquid-graviola fruit extract against human breast cancer (MCF-7) cell lines using flow cytometry techniques
title_full_unstemmed	Antiproliferative activity of ionic liquid-graviola fruit extract against human breast cancer (MCF-7) cell lines using flow cytometry techniques
title_sort	antiproliferative activity of ionic liquid-graviola fruit extract against human breast cancer (mcf-7) cell lines using flow cytometry techniques
publisher	Elsevier Ireland Ltd
publishDate	2019
url	http://irep.iium.edu.my/72405/ http://irep.iium.edu.my/72405/ http://irep.iium.edu.my/72405/ http://irep.iium.edu.my/72405/1/72405_Antiproliferative%20activity%20of%20ionic.pdf http://irep.iium.edu.my/72405/2/72405_Antiproliferative%20activity%20of%20ionic_SCOPUS.pdf http://irep.iium.edu.my/72405/3/72405_Antiproliferative%20activity%20of%20ionic_WOS.pdf
first_indexed	2023-09-18T21:42:36Z
last_indexed	2023-09-18T21:42:36Z
_version_	1777413239538188288

Antiproliferative activity of ionic liquid-graviola fruit extract against human breast cancer (MCF-7) cell lines using flow cytometry techniques

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