Development of integrated electrochemical-quartz crystal microbalance biosensor arrays: towards ultrasensitive, multiplexed and rapid point-of-care dengue detection

Dengue is an infectious mosquito-borne viral disease that affects approximately 50 million people annually worldwide and is prevalent mostly in the tropics. Severe cases of dengue can be fatal, making early detection and fast diagnosis crucial towards improving patient care and survival rates. Cur...

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Main Authors:	Zainuddin, Ahmad Anwar, Nordin, Anis Nurashikin, Asri, Mohd Afiq, Ab Rahim, Rosminazuin, Guines, Cyril, Chatras, Matthieu, Pothier, Arnaud, Mak, Wing Cheung
Format:	Conference or Workshop Item
Language:	English English
Published:	SciTePress 2019
Subjects:	T Technology (General) TK Electrical engineering. Electronics Nuclear engineering
Online Access:	http://irep.iium.edu.my/72664/ http://irep.iium.edu.my/72664/ http://irep.iium.edu.my/72664/3/72664_Development%20of%20integrated%20electrochemical-quartz_complete_final.pdf http://irep.iium.edu.my/72664/2/72664_Development%20of%20integrated%20electrochemical-quartz_scopus.pdf

id	iium-72664
recordtype	eprints
spelling	iium-726642019-06-17T02:41:05Z http://irep.iium.edu.my/72664/ Development of integrated electrochemical-quartz crystal microbalance biosensor arrays: towards ultrasensitive, multiplexed and rapid point-of-care dengue detection Zainuddin, Ahmad Anwar Nordin, Anis Nurashikin Asri, Mohd Afiq Ab Rahim, Rosminazuin Guines, Cyril Chatras, Matthieu Pothier, Arnaud Mak, Wing Cheung T Technology (General) TK Electrical engineering. Electronics Nuclear engineering Dengue is an infectious mosquito-borne viral disease that affects approximately 50 million people annually worldwide and is prevalent mostly in the tropics. Severe cases of dengue can be fatal, making early detection and fast diagnosis crucial towards improving patient care and survival rates. Currently, early detection can be achieved through detection of NS1 protein, using ELISA technique. Unfortunately, ELISA is an expensive method, making it unsuitable as a screening technique, especially in low-resource settings. In this work, we present a prototype device and its early validation studies, of an integrated electrochemical and mass-sensor for dengue NS1 antigen. The sensor is connected to open source mass-sensing software and hardware, OpenQCM which makes it easily portable. Having dual-measurement capabilities (mass and impedance) increases the sensitivity of the sensor. Preliminary studies suggest that the prototype could achieve ultralow limit of detection as low as 10 ng mL-1, dual-sensing cross-validation capability, portable size, sample-toanalysis time of less than 30 minutes, and parallelization of multiple assays. This work could lead to early and accurate dengue detection in routine point-of-care settings. SciTePress 2019-02 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/72664/3/72664_Development%20of%20integrated%20electrochemical-quartz_complete_final.pdf application/pdf en http://irep.iium.edu.my/72664/2/72664_Development%20of%20integrated%20electrochemical-quartz_scopus.pdf Zainuddin, Ahmad Anwar and Nordin, Anis Nurashikin and Asri, Mohd Afiq and Ab Rahim, Rosminazuin and Guines, Cyril and Chatras, Matthieu and Pothier, Arnaud and Mak, Wing Cheung (2019) Development of integrated electrochemical-quartz crystal microbalance biosensor arrays: towards ultrasensitive, multiplexed and rapid point-of-care dengue detection. In: 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019, 22 - 24 February 2019, Prague; Czech Republic. https://www.scitepress.org/PublicationsDetail.aspx?ID=YGsZnmARVu8%3d&t=1
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	T Technology (General) TK Electrical engineering. Electronics Nuclear engineering
spellingShingle	T Technology (General) TK Electrical engineering. Electronics Nuclear engineering Zainuddin, Ahmad Anwar Nordin, Anis Nurashikin Asri, Mohd Afiq Ab Rahim, Rosminazuin Guines, Cyril Chatras, Matthieu Pothier, Arnaud Mak, Wing Cheung Development of integrated electrochemical-quartz crystal microbalance biosensor arrays: towards ultrasensitive, multiplexed and rapid point-of-care dengue detection
description	Dengue is an infectious mosquito-borne viral disease that affects approximately 50 million people annually worldwide and is prevalent mostly in the tropics. Severe cases of dengue can be fatal, making early detection and fast diagnosis crucial towards improving patient care and survival rates. Currently, early detection can be achieved through detection of NS1 protein, using ELISA technique. Unfortunately, ELISA is an expensive method, making it unsuitable as a screening technique, especially in low-resource settings. In this work, we present a prototype device and its early validation studies, of an integrated electrochemical and mass-sensor for dengue NS1 antigen. The sensor is connected to open source mass-sensing software and hardware, OpenQCM which makes it easily portable. Having dual-measurement capabilities (mass and impedance) increases the sensitivity of the sensor. Preliminary studies suggest that the prototype could achieve ultralow limit of detection as low as 10 ng mL-1, dual-sensing cross-validation capability, portable size, sample-toanalysis time of less than 30 minutes, and parallelization of multiple assays. This work could lead to early and accurate dengue detection in routine point-of-care settings.
format	Conference or Workshop Item
author	Zainuddin, Ahmad Anwar Nordin, Anis Nurashikin Asri, Mohd Afiq Ab Rahim, Rosminazuin Guines, Cyril Chatras, Matthieu Pothier, Arnaud Mak, Wing Cheung
author_facet	Zainuddin, Ahmad Anwar Nordin, Anis Nurashikin Asri, Mohd Afiq Ab Rahim, Rosminazuin Guines, Cyril Chatras, Matthieu Pothier, Arnaud Mak, Wing Cheung
author_sort	Zainuddin, Ahmad Anwar
title	Development of integrated electrochemical-quartz crystal microbalance biosensor arrays: towards ultrasensitive, multiplexed and rapid point-of-care dengue detection
title_short	Development of integrated electrochemical-quartz crystal microbalance biosensor arrays: towards ultrasensitive, multiplexed and rapid point-of-care dengue detection
title_full	Development of integrated electrochemical-quartz crystal microbalance biosensor arrays: towards ultrasensitive, multiplexed and rapid point-of-care dengue detection
title_fullStr	Development of integrated electrochemical-quartz crystal microbalance biosensor arrays: towards ultrasensitive, multiplexed and rapid point-of-care dengue detection
title_full_unstemmed	Development of integrated electrochemical-quartz crystal microbalance biosensor arrays: towards ultrasensitive, multiplexed and rapid point-of-care dengue detection
title_sort	development of integrated electrochemical-quartz crystal microbalance biosensor arrays: towards ultrasensitive, multiplexed and rapid point-of-care dengue detection
publisher	SciTePress
publishDate	2019
url	http://irep.iium.edu.my/72664/ http://irep.iium.edu.my/72664/ http://irep.iium.edu.my/72664/3/72664_Development%20of%20integrated%20electrochemical-quartz_complete_final.pdf http://irep.iium.edu.my/72664/2/72664_Development%20of%20integrated%20electrochemical-quartz_scopus.pdf
first_indexed	2023-09-18T21:42:59Z
last_indexed	2023-09-18T21:42:59Z
_version_	1777413263836839936

Development of integrated electrochemical-quartz crystal microbalance biosensor arrays: towards ultrasensitive, multiplexed and rapid point-of-care dengue detection

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