Development and validation of UV-vis spectroscopic method of assay of carbamazepine in microparticles

Objective: This study aimed to develop a new, rapid, robust, effective, inexpensive, and accurate UV-Vis method for the quantification analysis of carbamazepine (CBZ) in the carbamazepine-loaded microparticles. Methods: CBZ was encapsulated in ethyl cellulose microparticles by a solvent evaporation...

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Main Authors:	Mawazi, Saeid Mezail, Ab. Hadi, Hazrina, Abdullah, Sinan Mohamed, Doolanea, Abd Almonem
Format:	Article
Language:	English English
Published:	Innovare Academics Sciences Pvt. Ltd 2019
Subjects:	RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology
Online Access:	http://irep.iium.edu.my/72710/ http://irep.iium.edu.my/72710/ http://irep.iium.edu.my/72710/ http://irep.iium.edu.my/72710/1/72710_Development%20and%20validation%20of%20UV-vis.pdf http://irep.iium.edu.my/72710/2/72710_Development%20and%20validation%20of%20UV-vis_SCOPUS.pdf

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recordtype	eprints
spelling	iium-727102019-11-24T14:59:21Z http://irep.iium.edu.my/72710/ Development and validation of UV-vis spectroscopic method of assay of carbamazepine in microparticles Mawazi, Saeid Mezail Ab. Hadi, Hazrina Abdullah, Sinan Mohamed Doolanea, Abd Almonem RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology Objective: This study aimed to develop a new, rapid, robust, effective, inexpensive, and accurate UV-Vis method for the quantification analysis of carbamazepine (CBZ) in the carbamazepine-loaded microparticles. Methods: CBZ was encapsulated in ethyl cellulose microparticles by a solvent evaporation method using polyvinyl alcohol (PVA) as a stabilizer. Methanol was used to dissolve CBZ followed by dilution with distilled water as diluent. CBZ drug, excipients, and microparticles were subjected to specificity, solution stability, linearity, precision and accuracy to confirm and ensure the validity of this method. Results: The results showed no interference from the excipients in the selected wavelength 286 nm. It was exhibited linearity in the range 2-12 μg/ml with R 2 = 0.9992. CBZ solution was stable during 24 h. Accuracy and precision were within the accepted limits (100±2%). All results were in accordance to the ICH-Q2 guideline. Conclusion: As a conclusion, CBZ could be quantified from loaded EC microparticles using UV-Vis spectrophotometer at 286 nm. Therefore, this method can be used for the quantification analysis of CBZ in CBZ-loaded microparticles can be utilized also as an alternative method to calculate CBZ in different dosage forms. © 2019 The Authors. Innovare Academics Sciences Pvt. Ltd 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/72710/1/72710_Development%20and%20validation%20of%20UV-vis.pdf application/pdf en http://irep.iium.edu.my/72710/2/72710_Development%20and%20validation%20of%20UV-vis_SCOPUS.pdf Mawazi, Saeid Mezail and Ab. Hadi, Hazrina and Abdullah, Sinan Mohamed and Doolanea, Abd Almonem (2019) Development and validation of UV-vis spectroscopic method of assay of carbamazepine in microparticles. International Journal of Applied Pharmaceutics, 11 (1). pp. 34-37. ISSN 0975-7058 https://innovareacademics.in/journals/index.php/ijap/article/view/26256/16803 10.22159/ijap.2019v11i1.26256
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology
spellingShingle	RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology Mawazi, Saeid Mezail Ab. Hadi, Hazrina Abdullah, Sinan Mohamed Doolanea, Abd Almonem Development and validation of UV-vis spectroscopic method of assay of carbamazepine in microparticles
description	Objective: This study aimed to develop a new, rapid, robust, effective, inexpensive, and accurate UV-Vis method for the quantification analysis of carbamazepine (CBZ) in the carbamazepine-loaded microparticles. Methods: CBZ was encapsulated in ethyl cellulose microparticles by a solvent evaporation method using polyvinyl alcohol (PVA) as a stabilizer. Methanol was used to dissolve CBZ followed by dilution with distilled water as diluent. CBZ drug, excipients, and microparticles were subjected to specificity, solution stability, linearity, precision and accuracy to confirm and ensure the validity of this method. Results: The results showed no interference from the excipients in the selected wavelength 286 nm. It was exhibited linearity in the range 2-12 μg/ml with R 2 = 0.9992. CBZ solution was stable during 24 h. Accuracy and precision were within the accepted limits (100±2%). All results were in accordance to the ICH-Q2 guideline. Conclusion: As a conclusion, CBZ could be quantified from loaded EC microparticles using UV-Vis spectrophotometer at 286 nm. Therefore, this method can be used for the quantification analysis of CBZ in CBZ-loaded microparticles can be utilized also as an alternative method to calculate CBZ in different dosage forms. © 2019 The Authors.
format	Article
author	Mawazi, Saeid Mezail Ab. Hadi, Hazrina Abdullah, Sinan Mohamed Doolanea, Abd Almonem
author_facet	Mawazi, Saeid Mezail Ab. Hadi, Hazrina Abdullah, Sinan Mohamed Doolanea, Abd Almonem
author_sort	Mawazi, Saeid Mezail
title	Development and validation of UV-vis spectroscopic method of assay of carbamazepine in microparticles
title_short	Development and validation of UV-vis spectroscopic method of assay of carbamazepine in microparticles
title_full	Development and validation of UV-vis spectroscopic method of assay of carbamazepine in microparticles
title_fullStr	Development and validation of UV-vis spectroscopic method of assay of carbamazepine in microparticles
title_full_unstemmed	Development and validation of UV-vis spectroscopic method of assay of carbamazepine in microparticles
title_sort	development and validation of uv-vis spectroscopic method of assay of carbamazepine in microparticles
publisher	Innovare Academics Sciences Pvt. Ltd
publishDate	2019
url	http://irep.iium.edu.my/72710/ http://irep.iium.edu.my/72710/ http://irep.iium.edu.my/72710/ http://irep.iium.edu.my/72710/1/72710_Development%20and%20validation%20of%20UV-vis.pdf http://irep.iium.edu.my/72710/2/72710_Development%20and%20validation%20of%20UV-vis_SCOPUS.pdf
first_indexed	2023-09-18T21:43:03Z
last_indexed	2023-09-18T21:43:03Z
_version_	1777413267993395200

Development and validation of UV-vis spectroscopic method of assay of carbamazepine in microparticles

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