Determination of cetirizine in human plasma and validation method using HPLC technique

This reseach project is dedicated to analyze cetirizine in human plasma by using HPLC qualitatively and quantitatively, to make a reliable bioanalysis of this drug in human plasma with a goal to utilise it in pharmacokinetics studies. In this research project, cetirizine was ex...

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Main Authors:	Zuhri, Siti Hazar, Azad, Abul Kalam, Mia, Abdur Rashid, Helaluddin, Abul Bashar Mohammed, Mohd. Nawi, Mohamed Sufian
Format:	Article
Language:	English
Published:	Journal of Drug Delivery and Therapeutics 2019
Subjects:	QD Chemistry RM Therapeutics. Pharmacology RS Pharmacy and materia medica RS403 Materia Medica-Pharmaceutical Chemistry
Online Access:	http://irep.iium.edu.my/72827/ http://irep.iium.edu.my/72827/ http://irep.iium.edu.my/72827/ http://irep.iium.edu.my/72827/1/JDDT%202019-Dr.%20Helal.pdf

id	iium-72827
recordtype	eprints
spelling	iium-728272019-06-26T01:59:32Z http://irep.iium.edu.my/72827/ Determination of cetirizine in human plasma and validation method using HPLC technique Zuhri, Siti Hazar Azad, Abul Kalam Mia, Abdur Rashid Helaluddin, Abul Bashar Mohammed Mohd. Nawi, Mohamed Sufian QD Chemistry RM Therapeutics. Pharmacology RS Pharmacy and materia medica RS403 Materia Medica-Pharmaceutical Chemistry This reseach project is dedicated to analyze cetirizine in human plasma by using HPLC qualitatively and quantitatively, to make a reliable bioanalysis of this drug in human plasma with a goal to utilise it in pharmacokinetics studies. In this research project, cetirizine was extracted from plasma using liquid-liquid extraction by dichloromethane and ethyl acetate. The results in terms of accuracy and precision are compared. For both of the methods, amlodipine was used as the internal standard. Separation was carried out by Phenomenex C18 chromato graphy column. The mobile phase used here is 35:65, v/v of Acetonitrile to 0.3% triethylamine (TEA) buffer fixed at pH 3 by phosphoric acid, with flow rate of 1mL/min. The column temperature was set at 30 °C. Variable wavelength detector (VWD) was used, and detection was set at 237 nm. The analysis was linear from 30 ng/mL to 500 ng/mL. Overall the recovery is more than 90% when using dichloromethane and more than 65% when using ethyl acetate. Journal of Drug Delivery and Therapeutics 2019-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/72827/1/JDDT%202019-Dr.%20Helal.pdf Zuhri, Siti Hazar and Azad, Abul Kalam and Mia, Abdur Rashid and Helaluddin, Abul Bashar Mohammed and Mohd. Nawi, Mohamed Sufian (2019) Determination of cetirizine in human plasma and validation method using HPLC technique. Journal of Drug Delivery and Therapeutics, 9 (2-S (March-April)). pp. 243-247. ISSN 2250-1177 http://jddtonline.info/index.php/jddt/article/view/2650/1930 10.22270/jddt.v9i2-s.2655
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry RM Therapeutics. Pharmacology RS Pharmacy and materia medica RS403 Materia Medica-Pharmaceutical Chemistry
spellingShingle	QD Chemistry RM Therapeutics. Pharmacology RS Pharmacy and materia medica RS403 Materia Medica-Pharmaceutical Chemistry Zuhri, Siti Hazar Azad, Abul Kalam Mia, Abdur Rashid Helaluddin, Abul Bashar Mohammed Mohd. Nawi, Mohamed Sufian Determination of cetirizine in human plasma and validation method using HPLC technique
description	This reseach project is dedicated to analyze cetirizine in human plasma by using HPLC qualitatively and quantitatively, to make a reliable bioanalysis of this drug in human plasma with a goal to utilise it in pharmacokinetics studies. In this research project, cetirizine was extracted from plasma using liquid-liquid extraction by dichloromethane and ethyl acetate. The results in terms of accuracy and precision are compared. For both of the methods, amlodipine was used as the internal standard. Separation was carried out by Phenomenex C18 chromato graphy column. The mobile phase used here is 35:65, v/v of Acetonitrile to 0.3% triethylamine (TEA) buffer fixed at pH 3 by phosphoric acid, with flow rate of 1mL/min. The column temperature was set at 30 °C. Variable wavelength detector (VWD) was used, and detection was set at 237 nm. The analysis was linear from 30 ng/mL to 500 ng/mL. Overall the recovery is more than 90% when using dichloromethane and more than 65% when using ethyl acetate.
format	Article
author	Zuhri, Siti Hazar Azad, Abul Kalam Mia, Abdur Rashid Helaluddin, Abul Bashar Mohammed Mohd. Nawi, Mohamed Sufian
author_facet	Zuhri, Siti Hazar Azad, Abul Kalam Mia, Abdur Rashid Helaluddin, Abul Bashar Mohammed Mohd. Nawi, Mohamed Sufian
author_sort	Zuhri, Siti Hazar
title	Determination of cetirizine in human plasma and validation method using HPLC technique
title_short	Determination of cetirizine in human plasma and validation method using HPLC technique
title_full	Determination of cetirizine in human plasma and validation method using HPLC technique
title_fullStr	Determination of cetirizine in human plasma and validation method using HPLC technique
title_full_unstemmed	Determination of cetirizine in human plasma and validation method using HPLC technique
title_sort	determination of cetirizine in human plasma and validation method using hplc technique
publisher	Journal of Drug Delivery and Therapeutics
publishDate	2019
url	http://irep.iium.edu.my/72827/ http://irep.iium.edu.my/72827/ http://irep.iium.edu.my/72827/ http://irep.iium.edu.my/72827/1/JDDT%202019-Dr.%20Helal.pdf
first_indexed	2023-09-18T21:43:15Z
last_indexed	2023-09-18T21:43:15Z
_version_	1777413280607764480

Determination of cetirizine in human plasma and validation method using HPLC technique

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