The effect of dental caries in children on the colonisation of Candida species
Objective: To determine the effect of caries towards colonization of Candida spp. Methods: To assess the colonization of Candida spp. in the oral cavity of paediatric patient, samples were obtained from 30 subjects aged five to six years old from International Islamic University Malaysia (IIUM) Dent...
| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/73639/ http://irep.iium.edu.my/73639/1/73639_The%20effect%20of%20dental%20caries%20in%20children%20_complete.pdf |
| Summary: | Objective: To determine the effect of caries towards colonization of Candida spp. Methods: To assess the colonization of Candida spp. in the oral cavity of paediatric patient, samples were obtained from 30 subjects aged five to six years old from International Islamic University Malaysia (IIUM) Dental Clinic and Adik Arif Kindergarten in Kuantan, Pahang, Malaysia. The samples were collected from buccal mucosa, palate and tooth surfaces using sterile swab. In addition, 10 mL of patient’s saliva suspension was also collected. Following that, the samples were inoculated aseptically on CHROMagar and incubated for 24 h to 48 h at 37 ºC. Finally, the colour, morphology and colony forming unit were determined. Results: This study has shown that no C. albicans was isolated from caries-free oral cavity. Meanwhile, 76% of children with caries possessed Candida spp. in their oral cavity with 65% of Candida spp. isolated was from the tooth surface. Only 35% of the total isolates were obtained from soft tissues including palatal and buccal mucosa. In addition, 82% and 67% of the yeast isolated from the tooth surface and buccal mucosa, respectively were C. albicans. Meanwhile, only C. albicans (1.2 0.5 x 105 cfu mL-1) was isolated from saliva of children with caries. Conclusions: Dental caries enhance the colonization of Candida spp. particularly C. albicans in children’s oral cavity. |
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