The effect of different composite on Candida albicans biofilm development

Introduction: Candida albicans is an opportunistic microorganism that inhabit oral cavity. It is the most common fungal pathogens isolated from the oral cavity and frequently cause superficial infections such as oral candidiasis. The objectives of the present study are to investigate the effect of c...

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Main Authors:	Nor Azemi, Siti Nor Humaira, Raja Sahrul Hishan, Raja Izzatun Nisa’, Muhamad Halil, Mohd Haikal, Arzmi, Mohd Hafiz
Format:	Conference or Workshop Item
Language:	English
Published:	2019
Subjects:	QR Microbiology RK Dentistry
Online Access:	http://irep.iium.edu.my/73645/ http://irep.iium.edu.my/73645/1/73685_The%20effect%20of%20different%20composite_complete.pdf

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recordtype	eprints
spelling	iium-736452019-09-30T08:31:37Z http://irep.iium.edu.my/73645/ The effect of different composite on Candida albicans biofilm development Nor Azemi, Siti Nor Humaira Raja Sahrul Hishan, Raja Izzatun Nisa’ Muhamad Halil, Mohd Haikal Arzmi, Mohd Hafiz QR Microbiology RK Dentistry Introduction: Candida albicans is an opportunistic microorganism that inhabit oral cavity. It is the most common fungal pathogens isolated from the oral cavity and frequently cause superficial infections such as oral candidiasis. The objectives of the present study are to investigate the effect of caries on C. albicans biofilm formation and to determine biofilm development of C. albicans on different types of composites with the hypotheses that caries isolate C. albicans form more biofilms compared to lab strain and that C. albicans form different cell number in biofilm grown in different composite. Materials and methods: To assess the biofilm forming ability of C. albicans ATCC 32354 and caries iso-late HNFC2 on different composites, 750 μL of YPD broth was pipetted into each well of 12-well plate. Following that 750 μL of C. albicans inoculum prepared in yeast peptone dextrose (YPD) broth was add-ed in the same well. Finally, sterile composite bead (SHOFU, DENTSPLY or SDI) was placed into each well aseptically and incubated at 37 °C for 24 h. Results: The present study has shown that C. albicans HNFC2 has significantly higher cell number on the composites compared to the ATCC (P < 0.05). Meanwhile, in comparison of the three types of compo-sites, DENTSPLY exhibited the least cell number in HNFC2 (7.7 x 10³ cells mL-1) and composite SHOFU was the highest (14.3 x 10³ cells mL-1). Conclusions: In conclusion, C. albicans form different cell number in biofilm grown on different com-posite, and caries isolate C. albicans form more biofilm on composite compared to lab strain. 2019-02-25 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/73645/1/73685_The%20effect%20of%20different%20composite_complete.pdf Nor Azemi, Siti Nor Humaira and Raja Sahrul Hishan, Raja Izzatun Nisa’ and Muhamad Halil, Mohd Haikal and Arzmi, Mohd Hafiz (2019) The effect of different composite on Candida albicans biofilm development. In: 8th Dental Student's Scientific Conference IIUM 2019, 25th February 2019, Kulliyyah of Dentistry, IIUM Kuantan Campus. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology RK Dentistry
spellingShingle	QR Microbiology RK Dentistry Nor Azemi, Siti Nor Humaira Raja Sahrul Hishan, Raja Izzatun Nisa’ Muhamad Halil, Mohd Haikal Arzmi, Mohd Hafiz The effect of different composite on Candida albicans biofilm development
description	Introduction: Candida albicans is an opportunistic microorganism that inhabit oral cavity. It is the most common fungal pathogens isolated from the oral cavity and frequently cause superficial infections such as oral candidiasis. The objectives of the present study are to investigate the effect of caries on C. albicans biofilm formation and to determine biofilm development of C. albicans on different types of composites with the hypotheses that caries isolate C. albicans form more biofilms compared to lab strain and that C. albicans form different cell number in biofilm grown in different composite. Materials and methods: To assess the biofilm forming ability of C. albicans ATCC 32354 and caries iso-late HNFC2 on different composites, 750 μL of YPD broth was pipetted into each well of 12-well plate. Following that 750 μL of C. albicans inoculum prepared in yeast peptone dextrose (YPD) broth was add-ed in the same well. Finally, sterile composite bead (SHOFU, DENTSPLY or SDI) was placed into each well aseptically and incubated at 37 °C for 24 h. Results: The present study has shown that C. albicans HNFC2 has significantly higher cell number on the composites compared to the ATCC (P < 0.05). Meanwhile, in comparison of the three types of compo-sites, DENTSPLY exhibited the least cell number in HNFC2 (7.7 x 10³ cells mL-1) and composite SHOFU was the highest (14.3 x 10³ cells mL-1). Conclusions: In conclusion, C. albicans form different cell number in biofilm grown on different com-posite, and caries isolate C. albicans form more biofilm on composite compared to lab strain.
format	Conference or Workshop Item
author	Nor Azemi, Siti Nor Humaira Raja Sahrul Hishan, Raja Izzatun Nisa’ Muhamad Halil, Mohd Haikal Arzmi, Mohd Hafiz
author_facet	Nor Azemi, Siti Nor Humaira Raja Sahrul Hishan, Raja Izzatun Nisa’ Muhamad Halil, Mohd Haikal Arzmi, Mohd Hafiz
author_sort	Nor Azemi, Siti Nor Humaira
title	The effect of different composite on Candida albicans biofilm development
title_short	The effect of different composite on Candida albicans biofilm development
title_full	The effect of different composite on Candida albicans biofilm development
title_fullStr	The effect of different composite on Candida albicans biofilm development
title_full_unstemmed	The effect of different composite on Candida albicans biofilm development
title_sort	effect of different composite on candida albicans biofilm development
publishDate	2019
url	http://irep.iium.edu.my/73645/ http://irep.iium.edu.my/73645/1/73685_The%20effect%20of%20different%20composite_complete.pdf
first_indexed	2023-09-18T21:44:24Z
last_indexed	2023-09-18T21:44:24Z
_version_	1777413352861990912

The effect of different composite on Candida albicans biofilm development

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