Inhibition of Burkholderia cenocepacia H111 quorum sensing system by environmental bacterial isolates

Burkholderia cenocepacia H111 is an opportunistic pathogen which is difficult to eradicate due to its virulence factors, specifically biofilm. The ability of B. cenocepacia to form biofilm is regulated by quorum sensing (QS) system using a signal molecule. Environtmental bacteria often showed the ab...

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Main Authors:	Wahjudi, Mariana, Kurniawan, W D, Gultom, M T, Artadana, Ida Bagus Made, Mohamad Puad, Noor Illi
Format:	Article
Language:	English English
Published:	IOP Publishing 2019
Subjects:	QR Microbiology T Technology (General)
Online Access:	http://irep.iium.edu.my/73655/ http://irep.iium.edu.my/73655/ http://irep.iium.edu.my/73655/ http://irep.iium.edu.my/73655/7/73655%20Inhibition%20of%20Burkholderia%20cenocepacia.pdf http://irep.iium.edu.my/73655/8/73655%20Inhibition%20of%20Burkholderia%20cenocepacia%20SCOPUS.pdf

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spelling	iium-736552019-09-11T07:59:36Z http://irep.iium.edu.my/73655/ Inhibition of Burkholderia cenocepacia H111 quorum sensing system by environmental bacterial isolates Wahjudi, Mariana Kurniawan, W D Gultom, M T Artadana, Ida Bagus Made Mohamad Puad, Noor Illi QR Microbiology T Technology (General) Burkholderia cenocepacia H111 is an opportunistic pathogen which is difficult to eradicate due to its virulence factors, specifically biofilm. The ability of B. cenocepacia to form biofilm is regulated by quorum sensing (QS) system using a signal molecule. Environtmental bacteria often showed the ability to inhibit the QS of other species because they have special and unique metabolites or enzymes. In previous research several environtmental bacteria was isolated from few places in Java Island. However, until now the potential of these bacteria have not been investigated yet. This study was conducted with the aim to investigate the effect of these environmental bacterial isolates, i.e. Bacillus sp., Stenotrophomonas maltophilia, Enterobacter ludwigii and Kosakonia radicincitans on quorum sensing system of B. cenocepacia H111, particularly on quantities of acyl homoserine lactones (AHLs) and biofilm that were produced. The quantity of AHL molecule is measured by biosensor assay using Escherichia coli JM109 pSB401 while the quantity of biofilm formation was quantified by crystal violet method. The result showed that supernatants of all these environtmental bacteria have no impact in decreasing of AHL nor biofilm. On the other hands, all the crude extracts showed a reduction either to AHL molecule and biofilm formation. The Bacillus sp. crude extract revealed the most reducing effect, with 84 % for AHL molecule and 71 % for biofilm. It can be concluded that crude extracts of all environmental bacteria have quorum quenching activity to B. cenocepacia H111 cells, specifically in reducing AHL levels and biofilm quantity. Crude extract from Bacillus sp. gave the highest inhibitory effect. IOP Publishing 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/73655/7/73655%20Inhibition%20of%20Burkholderia%20cenocepacia.pdf application/pdf en http://irep.iium.edu.my/73655/8/73655%20Inhibition%20of%20Burkholderia%20cenocepacia%20SCOPUS.pdf Wahjudi, Mariana and Kurniawan, W D and Gultom, M T and Artadana, Ida Bagus Made and Mohamad Puad, Noor Illi (2019) Inhibition of Burkholderia cenocepacia H111 quorum sensing system by environmental bacterial isolates. IOP Conference Series: Earth and Environmental Science, 293. pp. 1-9. ISSN 1755-1307 E-ISSN 1755-1315 https://iopscience.iop.org/article/10.1088/1755-1315/293/1/012028/pdf :10.1088/1755-1315/293/1/012028
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology T Technology (General)
spellingShingle	QR Microbiology T Technology (General) Wahjudi, Mariana Kurniawan, W D Gultom, M T Artadana, Ida Bagus Made Mohamad Puad, Noor Illi Inhibition of Burkholderia cenocepacia H111 quorum sensing system by environmental bacterial isolates
description	Burkholderia cenocepacia H111 is an opportunistic pathogen which is difficult to eradicate due to its virulence factors, specifically biofilm. The ability of B. cenocepacia to form biofilm is regulated by quorum sensing (QS) system using a signal molecule. Environtmental bacteria often showed the ability to inhibit the QS of other species because they have special and unique metabolites or enzymes. In previous research several environtmental bacteria was isolated from few places in Java Island. However, until now the potential of these bacteria have not been investigated yet. This study was conducted with the aim to investigate the effect of these environmental bacterial isolates, i.e. Bacillus sp., Stenotrophomonas maltophilia, Enterobacter ludwigii and Kosakonia radicincitans on quorum sensing system of B. cenocepacia H111, particularly on quantities of acyl homoserine lactones (AHLs) and biofilm that were produced. The quantity of AHL molecule is measured by biosensor assay using Escherichia coli JM109 pSB401 while the quantity of biofilm formation was quantified by crystal violet method. The result showed that supernatants of all these environtmental bacteria have no impact in decreasing of AHL nor biofilm. On the other hands, all the crude extracts showed a reduction either to AHL molecule and biofilm formation. The Bacillus sp. crude extract revealed the most reducing effect, with 84 % for AHL molecule and 71 % for biofilm. It can be concluded that crude extracts of all environmental bacteria have quorum quenching activity to B. cenocepacia H111 cells, specifically in reducing AHL levels and biofilm quantity. Crude extract from Bacillus sp. gave the highest inhibitory effect.
format	Article
author	Wahjudi, Mariana Kurniawan, W D Gultom, M T Artadana, Ida Bagus Made Mohamad Puad, Noor Illi
author_facet	Wahjudi, Mariana Kurniawan, W D Gultom, M T Artadana, Ida Bagus Made Mohamad Puad, Noor Illi
author_sort	Wahjudi, Mariana
title	Inhibition of Burkholderia cenocepacia H111 quorum sensing system by environmental bacterial isolates
title_short	Inhibition of Burkholderia cenocepacia H111 quorum sensing system by environmental bacterial isolates
title_full	Inhibition of Burkholderia cenocepacia H111 quorum sensing system by environmental bacterial isolates
title_fullStr	Inhibition of Burkholderia cenocepacia H111 quorum sensing system by environmental bacterial isolates
title_full_unstemmed	Inhibition of Burkholderia cenocepacia H111 quorum sensing system by environmental bacterial isolates
title_sort	inhibition of burkholderia cenocepacia h111 quorum sensing system by environmental bacterial isolates
publisher	IOP Publishing
publishDate	2019
url	http://irep.iium.edu.my/73655/ http://irep.iium.edu.my/73655/ http://irep.iium.edu.my/73655/ http://irep.iium.edu.my/73655/7/73655%20Inhibition%20of%20Burkholderia%20cenocepacia.pdf http://irep.iium.edu.my/73655/8/73655%20Inhibition%20of%20Burkholderia%20cenocepacia%20SCOPUS.pdf
first_indexed	2023-09-18T21:44:25Z
last_indexed	2023-09-18T21:44:25Z
_version_	1777413354064707584

Inhibition of Burkholderia cenocepacia H111 quorum sensing system by environmental bacterial isolates

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