Genomic determinants of specific virulent gene of blastocystis amoeboid stage as a marker in zoonoticpotential, biological and host-parasite relationships

Blastocystis sp. is an protozoan with 17 distinctive subtypes discovered in animals and humans worldwide. Unlike human, animal particularly cattle infected with Blastocystis are commonly healthy carriers and serve as a main reservoir in transmiting the infection to human.Thus, this study was aimed t...

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Main Author:	Mohammad, Mardhiah
Format:	Monograph
Language:	English
Published:	2019
Subjects:	Q Science (General) R Medicine (General)
Online Access:	http://irep.iium.edu.my/74329/ http://irep.iium.edu.my/74329/1/2.%20Project%20Report%20FRGS.pdf

id	iium-74329
recordtype	eprints
spelling	iium-743292019-12-01T02:56:35Z http://irep.iium.edu.my/74329/ Genomic determinants of specific virulent gene of blastocystis amoeboid stage as a marker in zoonoticpotential, biological and host-parasite relationships Mohammad, Mardhiah Q Science (General) R Medicine (General) Blastocystis sp. is an protozoan with 17 distinctive subtypes discovered in animals and humans worldwide. Unlike human, animal particularly cattle infected with Blastocystis are commonly healthy carriers and serve as a main reservoir in transmiting the infection to human.Thus, this study was aimed to determine the genotypic subtype distribution of Blastocystis sp. of cattle isolated from cattle and protease activities in the selected subtypes. A total of 120 faecal cattle sample were collected from three farms in Pahang, Malaysia for cultivation and microscopy identification. Later, DNA extracted from the faecal were subjected to genotyping analysis before the protease activity of three different subtypes were determined using Azocasein assay.The study found 30 out of 120 (25%) cattle infected with Blastocystis and vacuolar was the dominant morphological forms observed during cultivation. Besides, ST10 occurred predominantly along with another 5 subtypes (ST1, ST3, ST4, ST5 and ST14). Blastocystis ST3, ST5 and ST10 were found to mainly expressed cysteine protease with partially other proteases, which could responsible for the pathogenesis.In conclusion, moderate rates of Blastocystis infection with six different subtypes were identified in the cattle. This information is important in understanding host-parasite relationship associated with protease activity could serve as causative agents for diseases until further investigation were conducted. 2019-08-26 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/74329/1/2.%20Project%20Report%20FRGS.pdf Mohammad, Mardhiah (2019) Genomic determinants of specific virulent gene of blastocystis amoeboid stage as a marker in zoonoticpotential, biological and host-parasite relationships. Research Report. UNSPECIFIED. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) R Medicine (General)
spellingShingle	Q Science (General) R Medicine (General) Mohammad, Mardhiah Genomic determinants of specific virulent gene of blastocystis amoeboid stage as a marker in zoonoticpotential, biological and host-parasite relationships
description	Blastocystis sp. is an protozoan with 17 distinctive subtypes discovered in animals and humans worldwide. Unlike human, animal particularly cattle infected with Blastocystis are commonly healthy carriers and serve as a main reservoir in transmiting the infection to human.Thus, this study was aimed to determine the genotypic subtype distribution of Blastocystis sp. of cattle isolated from cattle and protease activities in the selected subtypes. A total of 120 faecal cattle sample were collected from three farms in Pahang, Malaysia for cultivation and microscopy identification. Later, DNA extracted from the faecal were subjected to genotyping analysis before the protease activity of three different subtypes were determined using Azocasein assay.The study found 30 out of 120 (25%) cattle infected with Blastocystis and vacuolar was the dominant morphological forms observed during cultivation. Besides, ST10 occurred predominantly along with another 5 subtypes (ST1, ST3, ST4, ST5 and ST14). Blastocystis ST3, ST5 and ST10 were found to mainly expressed cysteine protease with partially other proteases, which could responsible for the pathogenesis.In conclusion, moderate rates of Blastocystis infection with six different subtypes were identified in the cattle. This information is important in understanding host-parasite relationship associated with protease activity could serve as causative agents for diseases until further investigation were conducted.
format	Monograph
author	Mohammad, Mardhiah
author_facet	Mohammad, Mardhiah
author_sort	Mohammad, Mardhiah
title	Genomic determinants of specific virulent gene of blastocystis amoeboid stage as a marker in zoonoticpotential, biological and host-parasite relationships
title_short	Genomic determinants of specific virulent gene of blastocystis amoeboid stage as a marker in zoonoticpotential, biological and host-parasite relationships
title_full	Genomic determinants of specific virulent gene of blastocystis amoeboid stage as a marker in zoonoticpotential, biological and host-parasite relationships
title_fullStr	Genomic determinants of specific virulent gene of blastocystis amoeboid stage as a marker in zoonoticpotential, biological and host-parasite relationships
title_full_unstemmed	Genomic determinants of specific virulent gene of blastocystis amoeboid stage as a marker in zoonoticpotential, biological and host-parasite relationships
title_sort	genomic determinants of specific virulent gene of blastocystis amoeboid stage as a marker in zoonoticpotential, biological and host-parasite relationships
publishDate	2019
url	http://irep.iium.edu.my/74329/ http://irep.iium.edu.my/74329/1/2.%20Project%20Report%20FRGS.pdf
first_indexed	2023-09-18T21:45:18Z
last_indexed	2023-09-18T21:45:18Z
_version_	1777413409633992704

Genomic determinants of specific virulent gene of blastocystis amoeboid stage as a marker in zoonoticpotential, biological and host-parasite relationships

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