Hemolymph profiling reveals post bleeding stress in Tachypleus gigas
Horseshoe crabs amoebocyte cells degranulates to form a gel clot when in contact with endotoxins/Lipopolysaccharide (LPS) that are cellular constituents of gram-negative bacteria. This phenomenon is the basis of both Horseshoe crab immune system and detection of endotoxin in biologicals. Present...
| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/74599/ http://irep.iium.edu.my/74599/7/74599%20programme.pdf http://irep.iium.edu.my/74599/1/Hong%20Kong.pdf |
| Summary: | Horseshoe crabs amoebocyte cells degranulates to form a gel clot when in contact with
endotoxins/Lipopolysaccharide (LPS) that are cellular constituents of gram-negative bacteria.
This phenomenon is the basis of both Horseshoe crab immune system and detection of
endotoxin in biologicals. Present study investigates the quality of amoebocyte cells in
Tachypleus gigas pre and post bleeding under captivity. Wild and captive horseshoe crabs (5
months captivity) were bled in 6 anticoagulant formulations (A, B, C, D, E and F). Experiments
were carried out in triplicate using a total of 18 T. gigas (3 crabs for each anticoagulant
treatment) to determine cell viability and density. No profound difference in cell density
observed between captive and wild crabs with the mean value of 1.1×107 cells/mL and
1.167×107 cells/mL, respectively. while, the cell viability of captive group was significantly
lower than the wild crabs (P<0.001). Cell viability of captive group in anticoagulant A, B, C,
D, E, F were 40%, 52%, 58%,11%, 18%, 39%, respectively. Whilst, cell viability of wild crabs
were 80%, 84%, 86%, 36%, 45%, 54%, respectively. The anticoagulant formulations had
varying capabilities in maintaining cell viability with formula B and C being significantly
better than the rest (P<0.001), however, no significant difference between Formula B and C
was noted. This was attributed to the various components in the anticoagulant formulations
such as the buffering and chelating agent used. We conclude that the captivity has negative
effect on the amoebocyte viability. Anticoagulant formulation is crucial to determine the
hemolymph profiling of xiphosuran arthropods. |
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