Hemolymph profiling reveals post bleeding stress in Tachypleus gigas

Horseshoe crabs amoebocyte cells degranulates to form a gel clot when in contact with endotoxins/Lipopolysaccharide (LPS) that are cellular constituents of gram-negative bacteria. This phenomenon is the basis of both Horseshoe crab immune system and detection of endotoxin in biologicals. Present...

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Main Authors:	John, Akbar, Sheikh, Hassan I
Format:	Conference or Workshop Item
Language:	English English
Published:	2019
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/74599/ http://irep.iium.edu.my/74599/7/74599%20programme.pdf http://irep.iium.edu.my/74599/1/Hong%20Kong.pdf

id	iium-74599
recordtype	eprints
spelling	iium-745992019-10-03T01:49:10Z http://irep.iium.edu.my/74599/ Hemolymph profiling reveals post bleeding stress in Tachypleus gigas John, Akbar Sheikh, Hassan I Q Science (General) Horseshoe crabs amoebocyte cells degranulates to form a gel clot when in contact with endotoxins/Lipopolysaccharide (LPS) that are cellular constituents of gram-negative bacteria. This phenomenon is the basis of both Horseshoe crab immune system and detection of endotoxin in biologicals. Present study investigates the quality of amoebocyte cells in Tachypleus gigas pre and post bleeding under captivity. Wild and captive horseshoe crabs (5 months captivity) were bled in 6 anticoagulant formulations (A, B, C, D, E and F). Experiments were carried out in triplicate using a total of 18 T. gigas (3 crabs for each anticoagulant treatment) to determine cell viability and density. No profound difference in cell density observed between captive and wild crabs with the mean value of 1.1×107 cells/mL and 1.167×107 cells/mL, respectively. while, the cell viability of captive group was significantly lower than the wild crabs (P<0.001). Cell viability of captive group in anticoagulant A, B, C, D, E, F were 40%, 52%, 58%,11%, 18%, 39%, respectively. Whilst, cell viability of wild crabs were 80%, 84%, 86%, 36%, 45%, 54%, respectively. The anticoagulant formulations had varying capabilities in maintaining cell viability with formula B and C being significantly better than the rest (P<0.001), however, no significant difference between Formula B and C was noted. This was attributed to the various components in the anticoagulant formulations such as the buffering and chelating agent used. We conclude that the captivity has negative effect on the amoebocyte viability. Anticoagulant formulation is crucial to determine the hemolymph profiling of xiphosuran arthropods. 2019 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/74599/7/74599%20programme.pdf application/pdf en http://irep.iium.edu.my/74599/1/Hong%20Kong.pdf John, Akbar and Sheikh, Hassan I (2019) Hemolymph profiling reveals post bleeding stress in Tachypleus gigas. In: The Crustacean Society Mid-Year Meeting 2019, 26th-30th May 2019, Hong Kong. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) John, Akbar Sheikh, Hassan I Hemolymph profiling reveals post bleeding stress in Tachypleus gigas
description	Horseshoe crabs amoebocyte cells degranulates to form a gel clot when in contact with endotoxins/Lipopolysaccharide (LPS) that are cellular constituents of gram-negative bacteria. This phenomenon is the basis of both Horseshoe crab immune system and detection of endotoxin in biologicals. Present study investigates the quality of amoebocyte cells in Tachypleus gigas pre and post bleeding under captivity. Wild and captive horseshoe crabs (5 months captivity) were bled in 6 anticoagulant formulations (A, B, C, D, E and F). Experiments were carried out in triplicate using a total of 18 T. gigas (3 crabs for each anticoagulant treatment) to determine cell viability and density. No profound difference in cell density observed between captive and wild crabs with the mean value of 1.1×107 cells/mL and 1.167×107 cells/mL, respectively. while, the cell viability of captive group was significantly lower than the wild crabs (P<0.001). Cell viability of captive group in anticoagulant A, B, C, D, E, F were 40%, 52%, 58%,11%, 18%, 39%, respectively. Whilst, cell viability of wild crabs were 80%, 84%, 86%, 36%, 45%, 54%, respectively. The anticoagulant formulations had varying capabilities in maintaining cell viability with formula B and C being significantly better than the rest (P<0.001), however, no significant difference between Formula B and C was noted. This was attributed to the various components in the anticoagulant formulations such as the buffering and chelating agent used. We conclude that the captivity has negative effect on the amoebocyte viability. Anticoagulant formulation is crucial to determine the hemolymph profiling of xiphosuran arthropods.
format	Conference or Workshop Item
author	John, Akbar Sheikh, Hassan I
author_facet	John, Akbar Sheikh, Hassan I
author_sort	John, Akbar
title	Hemolymph profiling reveals post bleeding stress in Tachypleus gigas
title_short	Hemolymph profiling reveals post bleeding stress in Tachypleus gigas
title_full	Hemolymph profiling reveals post bleeding stress in Tachypleus gigas
title_fullStr	Hemolymph profiling reveals post bleeding stress in Tachypleus gigas
title_full_unstemmed	Hemolymph profiling reveals post bleeding stress in Tachypleus gigas
title_sort	hemolymph profiling reveals post bleeding stress in tachypleus gigas
publishDate	2019
url	http://irep.iium.edu.my/74599/ http://irep.iium.edu.my/74599/7/74599%20programme.pdf http://irep.iium.edu.my/74599/1/Hong%20Kong.pdf
first_indexed	2023-09-18T21:45:36Z
last_indexed	2023-09-18T21:45:36Z
_version_	1777413428752678912

Hemolymph profiling reveals post bleeding stress in Tachypleus gigas

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