Microbial hydrolytic enzymes: In silico studies between polar and tropical regions

Enzyme is important as biocatalyst for industrial and biotechnological applications. Cold active enzymes have showed many advantages compare to mesophilic enzymes. Their cold active and thermolabile characteristics have shown potential benefits in many industries. In silico characterization of hyd...

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Main Authors:	Abd Latip, Muhammad Asyraf, Abdul Hamid, Azzmer Azzar, Nordin, Noor Faizul Hadry
Format:	Article
Language:	English English English
Published:	Elsevier 2019
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/74627/ http://irep.iium.edu.my/74627/ http://irep.iium.edu.my/74627/ http://irep.iium.edu.my/74627/7/74627%20Microbial%20hydrolytic%20enzymes.pdf http://irep.iium.edu.my/74627/8/74627%20Microbial%20hydrolytic%20enzymes%20SCOPUS.pdf http://irep.iium.edu.my/74627/19/74627_Microbial%20hydrolytic%20enzymes_wos.pdf

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spelling	iium-746272019-11-20T09:37:09Z http://irep.iium.edu.my/74627/ Microbial hydrolytic enzymes: In silico studies between polar and tropical regions Abd Latip, Muhammad Asyraf Abdul Hamid, Azzmer Azzar Nordin, Noor Faizul Hadry QR Microbiology Enzyme is important as biocatalyst for industrial and biotechnological applications. Cold active enzymes have showed many advantages compare to mesophilic enzymes. Their cold active and thermolabile characteristics have shown potential benefits in many industries. In silico characterization of hydrolytic enzymes originated from polar and tropical regions was conducted. Amino acid sequences and molecular structures of mesophilic and psychrophilic bacterial enzyme homologues were compared thoroughly. Amino acid sequences from these two homologs do not showed any extraordinary differences. Overall, protein folds were highly similar when psychrophilic homologies were matched with mesophilic homologies. Active site residues located in catalytic domain of both psychrophilic and mesophilic enzymes were highly conserved. Thus, the hydrolytic mechanisms of these cold active enzymes still remain similar to mesophilic enzymes. However, the differences were indicated between these two enzymes at the substrate-binding sites. Cold active enzymes showed unique loop conformations and smaller side chains at the entrances. These characteristics provided larger active site of the enzymes. Larger entrance of active site was expected to stipulate substrates binding and products exiting with lower energy consumption. This part of the enzymes indicated one of the important features of cold active enzyme to work efficiently at lower temperature. Based on this in silico study, cold active enzymes have much more advantages compared to the mesophilic enzymes that made them valuable to be further researched and applied at industrial level. Elsevier 2019-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/74627/7/74627%20Microbial%20hydrolytic%20enzymes.pdf application/pdf en http://irep.iium.edu.my/74627/8/74627%20Microbial%20hydrolytic%20enzymes%20SCOPUS.pdf application/pdf en http://irep.iium.edu.my/74627/19/74627_Microbial%20hydrolytic%20enzymes_wos.pdf Abd Latip, Muhammad Asyraf and Abdul Hamid, Azzmer Azzar and Nordin, Noor Faizul Hadry (2019) Microbial hydrolytic enzymes: In silico studies between polar and tropical regions. Polar Science, 20 (part 1). pp. 9-18. ISSN 1873-9652 https://www.sciencedirect.com/science/article/abs/pii/S187396521830094X 10.1016/j.polar.2019.04.003
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QR Microbiology
spellingShingle	QR Microbiology Abd Latip, Muhammad Asyraf Abdul Hamid, Azzmer Azzar Nordin, Noor Faizul Hadry Microbial hydrolytic enzymes: In silico studies between polar and tropical regions
description	Enzyme is important as biocatalyst for industrial and biotechnological applications. Cold active enzymes have showed many advantages compare to mesophilic enzymes. Their cold active and thermolabile characteristics have shown potential benefits in many industries. In silico characterization of hydrolytic enzymes originated from polar and tropical regions was conducted. Amino acid sequences and molecular structures of mesophilic and psychrophilic bacterial enzyme homologues were compared thoroughly. Amino acid sequences from these two homologs do not showed any extraordinary differences. Overall, protein folds were highly similar when psychrophilic homologies were matched with mesophilic homologies. Active site residues located in catalytic domain of both psychrophilic and mesophilic enzymes were highly conserved. Thus, the hydrolytic mechanisms of these cold active enzymes still remain similar to mesophilic enzymes. However, the differences were indicated between these two enzymes at the substrate-binding sites. Cold active enzymes showed unique loop conformations and smaller side chains at the entrances. These characteristics provided larger active site of the enzymes. Larger entrance of active site was expected to stipulate substrates binding and products exiting with lower energy consumption. This part of the enzymes indicated one of the important features of cold active enzyme to work efficiently at lower temperature. Based on this in silico study, cold active enzymes have much more advantages compared to the mesophilic enzymes that made them valuable to be further researched and applied at industrial level.
format	Article
author	Abd Latip, Muhammad Asyraf Abdul Hamid, Azzmer Azzar Nordin, Noor Faizul Hadry
author_facet	Abd Latip, Muhammad Asyraf Abdul Hamid, Azzmer Azzar Nordin, Noor Faizul Hadry
author_sort	Abd Latip, Muhammad Asyraf
title	Microbial hydrolytic enzymes: In silico studies between polar and tropical regions
title_short	Microbial hydrolytic enzymes: In silico studies between polar and tropical regions
title_full	Microbial hydrolytic enzymes: In silico studies between polar and tropical regions
title_fullStr	Microbial hydrolytic enzymes: In silico studies between polar and tropical regions
title_full_unstemmed	Microbial hydrolytic enzymes: In silico studies between polar and tropical regions
title_sort	microbial hydrolytic enzymes: in silico studies between polar and tropical regions
publisher	Elsevier
publishDate	2019
url	http://irep.iium.edu.my/74627/ http://irep.iium.edu.my/74627/ http://irep.iium.edu.my/74627/ http://irep.iium.edu.my/74627/7/74627%20Microbial%20hydrolytic%20enzymes.pdf http://irep.iium.edu.my/74627/8/74627%20Microbial%20hydrolytic%20enzymes%20SCOPUS.pdf http://irep.iium.edu.my/74627/19/74627_Microbial%20hydrolytic%20enzymes_wos.pdf
first_indexed	2023-09-18T21:45:39Z
last_indexed	2023-09-18T21:45:39Z
_version_	1777413431719100416

Microbial hydrolytic enzymes: In silico studies between polar and tropical regions

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