Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain
It is important to know the thermal and storage behavior of produced protein for the purpose of storage, transport and shelve life during industrial application. Differential Scanning Calorimetry (DSC) is used to measure the thermal and storage stability of recombinant bromelain produced i...
| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/7470/ http://irep.iium.edu.my/7470/ http://irep.iium.edu.my/7470/1/10.BPE1_pg414-460_7470.pdf |
| Summary: | It is important to know the thermal and storage
behavior of produced protein for the purpose of storage,
transport and shelve life during industrial application.
Differential Scanning Calorimetry (DSC) is used to
measure the thermal and storage stability of recombinant
bromelain produced in our laboratory. Recombinant
bromelain thermal and storage stability were measured
and compared to the commercial bromelain. The purified
recombinant bromelain was powderized using freeze
drying method and its transition midpoint, crystalline and
enthalpy were recorded. Recombinant bromelain is more
stable than commercial bromelain at higher temperature
but the stability reduced after 7 days of storage at 4oC.
Moreover, its activity is lower than commercial at day 1
and 7. Higher energy is needed to break the bond between
amino acid chains in recombinant bromelain showing by
the enthalpy recorded suggesting that recombinant
bromelain has good protein structure and conformation
compared to commercial. |
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