Cyclic voltammetric and electrical impedance spectroscopy studies of graphene- and conductive polymer–based enzyme electrodes

Screen-printed carbon electrodes (SPCEs), modified with graphene oxide and poly(3,4-ethylenedioxythiophene):polystyrenesulfonic acid (GO-PEDOT:PSS/SPCEs), and SPCEs modified with partially reduced GO and PEDOT:PSS (prGO-PEDOT:PSS/SPCEs) were studied for electrochemical transduction. Randles-Sevcik a...

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Main Authors:	Ismail, Nur Alya Batrisya, Ramli, Nurul Izzati, Abd-Wahab, Firdaus, Wan Salim, Wan Wardatul Amani
Format:	Article
Language:	English English English
Published:	Universiti Tun Hussein Onn Malaysia 2019
Subjects:	QD Chemistry TP155 Chemical engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/74856/ http://irep.iium.edu.my/74856/ http://irep.iium.edu.my/74856/1/74856_Cyclic%20Voltammetric%20and%20Electrical%20Impedance.pdf http://irep.iium.edu.my/74856/2/74856_Cyclic%20Voltammetric%20and%20Electrical%20Impedance_WOS.pdf http://irep.iium.edu.my/74856/13/74856_Cyclic%20voltammetric%20and%20electrical%20impedance%20spectroscopy%20studies%20of%20graphene-%20and%20conductive%20polymer-based%20enzyme%20electrodes_Scopus.pdf

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spelling	iium-748562020-03-02T02:04:53Z http://irep.iium.edu.my/74856/ Cyclic voltammetric and electrical impedance spectroscopy studies of graphene- and conductive polymer–based enzyme electrodes Ismail, Nur Alya Batrisya Ramli, Nurul Izzati Abd-Wahab, Firdaus Wan Salim, Wan Wardatul Amani QD Chemistry TP155 Chemical engineering TP248.13 Biotechnology Screen-printed carbon electrodes (SPCEs), modified with graphene oxide and poly(3,4-ethylenedioxythiophene):polystyrenesulfonic acid (GO-PEDOT:PSS/SPCEs), and SPCEs modified with partially reduced GO and PEDOT:PSS (prGO-PEDOT:PSS/SPCEs) were studied for electrochemical transduction. Randles-Sevcik analysis showed that the prGO-PEDOT:PSS/SPCE has a higher effective surface area of 219.3 μm2 in comparison to the unreduced GO-PEDOT:PSS/SPCE (87.0 μm2) and the bare SPCE (71.7 μm2). Using electrical impedance spectroscopy (EIS), we determined that the prGO-PEDOT:PSS/SPCE has a lower charge-transfer resistance (Rct) of 163.82 Ω in comparison to the GO-PEDOT:PSS/SPCE (427.87 Ω) and the bare SPCE (13.31 kΩ). Glucose oxidase (GOx) was immobilized on all electrode types, including GO/SPCE as additional control and tested with low (0.2 mM), intermediate (0.6 mM), and high (1 mM) glucose concentrations. GOx/GO/SPCEs showed the largest change in anodic peak current (Ipa), 8.5, 7.5 and 4.9 μA for low, intermediate, and high glucose concentrations, respectively. Interestingly, GOx/prGO-PEDOT:PSS/SPCEs have no change in both anodic and cathodic peak current, although they exhibit better redox capability, while GOx/SPCEs have very low Ipa. The results show that the high effective surface area and low charge-transfer resistance (Rct) of prGO-PEDOT:PSS/SPCEs do not necessarily result in a sensitive glucose sensor in cases where immobilization of enzymes on the material can affect electron transfer. Universiti Tun Hussein Onn Malaysia 2019-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/74856/1/74856_Cyclic%20Voltammetric%20and%20Electrical%20Impedance.pdf application/pdf en http://irep.iium.edu.my/74856/2/74856_Cyclic%20Voltammetric%20and%20Electrical%20Impedance_WOS.pdf application/pdf en http://irep.iium.edu.my/74856/13/74856_Cyclic%20voltammetric%20and%20electrical%20impedance%20spectroscopy%20studies%20of%20graphene-%20and%20conductive%20polymer-based%20enzyme%20electrodes_Scopus.pdf Ismail, Nur Alya Batrisya and Ramli, Nurul Izzati and Abd-Wahab, Firdaus and Wan Salim, Wan Wardatul Amani (2019) Cyclic voltammetric and electrical impedance spectroscopy studies of graphene- and conductive polymer–based enzyme electrodes. International Journal of Integrated Engineering, 11 (3). pp. 70-80. ISSN 2229-838X E-ISSN 2600-7916 https://publisher.uthm.edu.my/ojs/index.php/ijie/article/view/4541/2943
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QD Chemistry TP155 Chemical engineering TP248.13 Biotechnology
spellingShingle	QD Chemistry TP155 Chemical engineering TP248.13 Biotechnology Ismail, Nur Alya Batrisya Ramli, Nurul Izzati Abd-Wahab, Firdaus Wan Salim, Wan Wardatul Amani Cyclic voltammetric and electrical impedance spectroscopy studies of graphene- and conductive polymer–based enzyme electrodes
description	Screen-printed carbon electrodes (SPCEs), modified with graphene oxide and poly(3,4-ethylenedioxythiophene):polystyrenesulfonic acid (GO-PEDOT:PSS/SPCEs), and SPCEs modified with partially reduced GO and PEDOT:PSS (prGO-PEDOT:PSS/SPCEs) were studied for electrochemical transduction. Randles-Sevcik analysis showed that the prGO-PEDOT:PSS/SPCE has a higher effective surface area of 219.3 μm2 in comparison to the unreduced GO-PEDOT:PSS/SPCE (87.0 μm2) and the bare SPCE (71.7 μm2). Using electrical impedance spectroscopy (EIS), we determined that the prGO-PEDOT:PSS/SPCE has a lower charge-transfer resistance (Rct) of 163.82 Ω in comparison to the GO-PEDOT:PSS/SPCE (427.87 Ω) and the bare SPCE (13.31 kΩ). Glucose oxidase (GOx) was immobilized on all electrode types, including GO/SPCE as additional control and tested with low (0.2 mM), intermediate (0.6 mM), and high (1 mM) glucose concentrations. GOx/GO/SPCEs showed the largest change in anodic peak current (Ipa), 8.5, 7.5 and 4.9 μA for low, intermediate, and high glucose concentrations, respectively. Interestingly, GOx/prGO-PEDOT:PSS/SPCEs have no change in both anodic and cathodic peak current, although they exhibit better redox capability, while GOx/SPCEs have very low Ipa. The results show that the high effective surface area and low charge-transfer resistance (Rct) of prGO-PEDOT:PSS/SPCEs do not necessarily result in a sensitive glucose sensor in cases where immobilization of enzymes on the material can affect electron transfer.
format	Article
author	Ismail, Nur Alya Batrisya Ramli, Nurul Izzati Abd-Wahab, Firdaus Wan Salim, Wan Wardatul Amani
author_facet	Ismail, Nur Alya Batrisya Ramli, Nurul Izzati Abd-Wahab, Firdaus Wan Salim, Wan Wardatul Amani
author_sort	Ismail, Nur Alya Batrisya
title	Cyclic voltammetric and electrical impedance spectroscopy studies of graphene- and conductive polymer–based enzyme electrodes
title_short	Cyclic voltammetric and electrical impedance spectroscopy studies of graphene- and conductive polymer–based enzyme electrodes
title_full	Cyclic voltammetric and electrical impedance spectroscopy studies of graphene- and conductive polymer–based enzyme electrodes
title_fullStr	Cyclic voltammetric and electrical impedance spectroscopy studies of graphene- and conductive polymer–based enzyme electrodes
title_full_unstemmed	Cyclic voltammetric and electrical impedance spectroscopy studies of graphene- and conductive polymer–based enzyme electrodes
title_sort	cyclic voltammetric and electrical impedance spectroscopy studies of graphene- and conductive polymer–based enzyme electrodes
publisher	Universiti Tun Hussein Onn Malaysia
publishDate	2019
url	http://irep.iium.edu.my/74856/ http://irep.iium.edu.my/74856/ http://irep.iium.edu.my/74856/1/74856_Cyclic%20Voltammetric%20and%20Electrical%20Impedance.pdf http://irep.iium.edu.my/74856/2/74856_Cyclic%20Voltammetric%20and%20Electrical%20Impedance_WOS.pdf http://irep.iium.edu.my/74856/13/74856_Cyclic%20voltammetric%20and%20electrical%20impedance%20spectroscopy%20studies%20of%20graphene-%20and%20conductive%20polymer-based%20enzyme%20electrodes_Scopus.pdf
first_indexed	2023-09-18T21:45:55Z
last_indexed	2023-09-18T21:45:55Z
_version_	1777413448658845696

Cyclic voltammetric and electrical impedance spectroscopy studies of graphene- and conductive polymer–based enzyme electrodes

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