Structural and functional properties of neocartilage construct engineered in poly (Lactic-co-Glycolic Acid) (PLGA) based scaffolds

The study aims to assess the structural and functional properties of in vitro three-dimensional (3D) PLGA-based hybrid scaffolds seeded with chondrocytes, particularly in terms of the production of specific cartilaginous extracellular matrix (ECM). The PLGA scaffolds were incorporated with ateloco...

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Main Authors:	Md Ali @ Tahir, Aisyah Hanani, Mohamed Amin, Muhammad Azri Ifwat, Noor Azmi, Azran Azhim, Sha'ban, Munirah
Format:	Article
Language:	English English
Published:	UTHM Press 2019
Subjects:	TA164 Bioengineering
Online Access:	http://irep.iium.edu.my/74944/ http://irep.iium.edu.my/74944/ http://irep.iium.edu.my/74944/ http://irep.iium.edu.my/74944/1/IJIE%20AISYAH%20HANANI.pdf http://irep.iium.edu.my/74944/6/74944_Structural%20and%20functional%20properties%20of%20neocartilage%20construct%20engineered%20in%20poly%20%28Lactic-co-Glycolic%20Acid%29%20%28PLGA%29%20based%20Scaffolds_Scopus.pdf

id	iium-74944
recordtype	eprints
spelling	iium-749442020-02-24T07:41:42Z http://irep.iium.edu.my/74944/ Structural and functional properties of neocartilage construct engineered in poly (Lactic-co-Glycolic Acid) (PLGA) based scaffolds Md Ali @ Tahir, Aisyah Hanani Mohamed Amin, Muhammad Azri Ifwat Noor Azmi, Azran Azhim Sha'ban, Munirah TA164 Bioengineering The study aims to assess the structural and functional properties of in vitro three-dimensional (3D) PLGA-based hybrid scaffolds seeded with chondrocytes, particularly in terms of the production of specific cartilaginous extracellular matrix (ECM). The PLGA scaffolds were incorporated with atelocollagen and/or fresh fibrin and assigned to four groups; PLGA only as control, PLGA-fibrin (PF), PLGA-atelocollagen (PA) and PLGA-atelocollagen-fibrin (PAF). The resulting PLGA hybrid scaffolds were characterized based on gross appearance, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy analysis, porosity and swelling tests as well as cytocompatibility analysis using cell proliferation (MTT) assay. All scaffolds seeded with cells were cultured for three weeks in vitro. Macroscopic changes were recorded using photographs. Microscopic evaluation of ‘cells-scaffolds’ construct was done using Haematoxylin and Eosin (H&E), Safranin O, Alcian Blue, Toluidine Blue and scanning electron microscopy (SEM). The production of cartilage specific ECM was measured using sulphated glycosaminoglycan (sGAG) assay. Based on physical characterizations, PLGAbased hybrid scaffolds have been successfully manufactured and showed no cytocompatibility issues. The PAF exhibited cartilaginous tissue morphology better than other scaffold groups, grossly and microscopically. On SEM, the presence of branching fibers that produce a web-like network on the surface of PLGA-based hybrid scaffolds indicated ECM secretion. This is supported by the manifestation of glycosaminoglycan and as well as proteoglycan through histology and sGAG assay. This present study indicated that PLGA-based hybrid scaffolds promote formation of neocartilage in vitro. UTHM Press 2019-09-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/74944/1/IJIE%20AISYAH%20HANANI.pdf application/pdf en http://irep.iium.edu.my/74944/6/74944_Structural%20and%20functional%20properties%20of%20neocartilage%20construct%20engineered%20in%20poly%20%28Lactic-co-Glycolic%20Acid%29%20%28PLGA%29%20based%20Scaffolds_Scopus.pdf Md Ali @ Tahir, Aisyah Hanani and Mohamed Amin, Muhammad Azri Ifwat and Noor Azmi, Azran Azhim and Sha'ban, Munirah (2019) Structural and functional properties of neocartilage construct engineered in poly (Lactic-co-Glycolic Acid) (PLGA) based scaffolds. International Journal of Integrated Engineering, 11 (3). pp. 109-118. ISSN 2229-838X E-ISSN 2600-7916 https://publisher.uthm.edu.my/ojs/index.php/ijie/article/view/4545/2947 10.30880/ijie.2019.11.03.012
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TA164 Bioengineering
spellingShingle	TA164 Bioengineering Md Ali @ Tahir, Aisyah Hanani Mohamed Amin, Muhammad Azri Ifwat Noor Azmi, Azran Azhim Sha'ban, Munirah Structural and functional properties of neocartilage construct engineered in poly (Lactic-co-Glycolic Acid) (PLGA) based scaffolds
description	The study aims to assess the structural and functional properties of in vitro three-dimensional (3D) PLGA-based hybrid scaffolds seeded with chondrocytes, particularly in terms of the production of specific cartilaginous extracellular matrix (ECM). The PLGA scaffolds were incorporated with atelocollagen and/or fresh fibrin and assigned to four groups; PLGA only as control, PLGA-fibrin (PF), PLGA-atelocollagen (PA) and PLGA-atelocollagen-fibrin (PAF). The resulting PLGA hybrid scaffolds were characterized based on gross appearance, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy analysis, porosity and swelling tests as well as cytocompatibility analysis using cell proliferation (MTT) assay. All scaffolds seeded with cells were cultured for three weeks in vitro. Macroscopic changes were recorded using photographs. Microscopic evaluation of ‘cells-scaffolds’ construct was done using Haematoxylin and Eosin (H&E), Safranin O, Alcian Blue, Toluidine Blue and scanning electron microscopy (SEM). The production of cartilage specific ECM was measured using sulphated glycosaminoglycan (sGAG) assay. Based on physical characterizations, PLGAbased hybrid scaffolds have been successfully manufactured and showed no cytocompatibility issues. The PAF exhibited cartilaginous tissue morphology better than other scaffold groups, grossly and microscopically. On SEM, the presence of branching fibers that produce a web-like network on the surface of PLGA-based hybrid scaffolds indicated ECM secretion. This is supported by the manifestation of glycosaminoglycan and as well as proteoglycan through histology and sGAG assay. This present study indicated that PLGA-based hybrid scaffolds promote formation of neocartilage in vitro.
format	Article
author	Md Ali @ Tahir, Aisyah Hanani Mohamed Amin, Muhammad Azri Ifwat Noor Azmi, Azran Azhim Sha'ban, Munirah
author_facet	Md Ali @ Tahir, Aisyah Hanani Mohamed Amin, Muhammad Azri Ifwat Noor Azmi, Azran Azhim Sha'ban, Munirah
author_sort	Md Ali @ Tahir, Aisyah Hanani
title	Structural and functional properties of neocartilage construct engineered in poly (Lactic-co-Glycolic Acid) (PLGA) based scaffolds
title_short	Structural and functional properties of neocartilage construct engineered in poly (Lactic-co-Glycolic Acid) (PLGA) based scaffolds
title_full	Structural and functional properties of neocartilage construct engineered in poly (Lactic-co-Glycolic Acid) (PLGA) based scaffolds
title_fullStr	Structural and functional properties of neocartilage construct engineered in poly (Lactic-co-Glycolic Acid) (PLGA) based scaffolds
title_full_unstemmed	Structural and functional properties of neocartilage construct engineered in poly (Lactic-co-Glycolic Acid) (PLGA) based scaffolds
title_sort	structural and functional properties of neocartilage construct engineered in poly (lactic-co-glycolic acid) (plga) based scaffolds
publisher	UTHM Press
publishDate	2019
url	http://irep.iium.edu.my/74944/ http://irep.iium.edu.my/74944/ http://irep.iium.edu.my/74944/ http://irep.iium.edu.my/74944/1/IJIE%20AISYAH%20HANANI.pdf http://irep.iium.edu.my/74944/6/74944_Structural%20and%20functional%20properties%20of%20neocartilage%20construct%20engineered%20in%20poly%20%28Lactic-co-Glycolic%20Acid%29%20%28PLGA%29%20based%20Scaffolds_Scopus.pdf
first_indexed	2023-09-18T21:46:03Z
last_indexed	2023-09-18T21:46:03Z
_version_	1777413457331617792

Structural and functional properties of neocartilage construct engineered in poly (Lactic-co-Glycolic Acid) (PLGA) based scaffolds

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