Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans

Candida albicans is one of the common causes for oral candidiasis worldwide. The proliferation of antifungal-resistant C. albicans has become a major concern. This study was carried out to evaluate activated virgin coconut oil (AVCO) and crude extract of virgin coconut oil (VCO) as new antifungal ag...

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Main Authors:	Mukhtar, Nor Izzah, Abllah, Zurainie, M., Azrul Naim, Shahdan, Intan Azura, Long, Kamariah, Haron, Ummi Aqilah
Format:	Article
Language:	English English English
Published:	2019
Subjects:	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
Online Access:	http://irep.iium.edu.my/75103/ http://irep.iium.edu.my/75103/ http://irep.iium.edu.my/75103/1/MJAS%202019.pdf http://irep.iium.edu.my/75103/3/letter%20from%20publisher.pdf http://irep.iium.edu.my/75103/9/75103_Application%20of%20direct%20fluorescence-based%20live%20or%20dead%20staining%20for%20assessment%20of%20antifungal%20activity%20of%20coconut%20oil%20against%20candida%20albicans_Scopus.pdf

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spelling	iium-751032020-02-28T07:31:42Z http://irep.iium.edu.my/75103/ Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans Mukhtar, Nor Izzah Abllah, Zurainie M., Azrul Naim Shahdan, Intan Azura Long, Kamariah Haron, Ummi Aqilah RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works Candida albicans is one of the common causes for oral candidiasis worldwide. The proliferation of antifungal-resistant C. albicans has become a major concern. This study was carried out to evaluate activated virgin coconut oil (AVCO) and crude extract of virgin coconut oil (VCO) as new antifungal agents for treatment of oral candidiasis. C. albicans viability was determined using LIVE/DEAD bacterial viability kit. C. albicans cells were grown in yeast peptone dextrose (YPD) broth overnight. The fungus was treated with AVCO and VCO at the concentration of minimum fungicidal concentration (MFC) of 6.24 mg/mL and incubated for three different time points (1, 2, and 3 h). To evaluate the viability of C. albicans, SYTO 9 and propidium iodide (PI) staining were used and the cells were observed using fluorescence microscopy. C. albicans treated with AVCO showed more dead cells compared to cells treated with VCO. The data indicate that exposure of C. albicans to AVCO was the most inhibitory to its growth (p < 0.01). 2019-08-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/75103/1/MJAS%202019.pdf application/pdf en http://irep.iium.edu.my/75103/3/letter%20from%20publisher.pdf application/pdf en http://irep.iium.edu.my/75103/9/75103_Application%20of%20direct%20fluorescence-based%20live%20or%20dead%20staining%20for%20assessment%20of%20antifungal%20activity%20of%20coconut%20oil%20against%20candida%20albicans_Scopus.pdf Mukhtar, Nor Izzah and Abllah, Zurainie and M., Azrul Naim and Shahdan, Intan Azura and Long, Kamariah and Haron, Ummi Aqilah (2019) Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans. Malaysian Journal of Analytical Sciences, 23 (5). pp. 1-6. ISSN 1394-2506 (In Press) https://doi.org/10.17576/mjas-2019-2305-zz
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
spellingShingle	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works Mukhtar, Nor Izzah Abllah, Zurainie M., Azrul Naim Shahdan, Intan Azura Long, Kamariah Haron, Ummi Aqilah Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans
description	Candida albicans is one of the common causes for oral candidiasis worldwide. The proliferation of antifungal-resistant C. albicans has become a major concern. This study was carried out to evaluate activated virgin coconut oil (AVCO) and crude extract of virgin coconut oil (VCO) as new antifungal agents for treatment of oral candidiasis. C. albicans viability was determined using LIVE/DEAD bacterial viability kit. C. albicans cells were grown in yeast peptone dextrose (YPD) broth overnight. The fungus was treated with AVCO and VCO at the concentration of minimum fungicidal concentration (MFC) of 6.24 mg/mL and incubated for three different time points (1, 2, and 3 h). To evaluate the viability of C. albicans, SYTO 9 and propidium iodide (PI) staining were used and the cells were observed using fluorescence microscopy. C. albicans treated with AVCO showed more dead cells compared to cells treated with VCO. The data indicate that exposure of C. albicans to AVCO was the most inhibitory to its growth (p < 0.01).
format	Article
author	Mukhtar, Nor Izzah Abllah, Zurainie M., Azrul Naim Shahdan, Intan Azura Long, Kamariah Haron, Ummi Aqilah
author_facet	Mukhtar, Nor Izzah Abllah, Zurainie M., Azrul Naim Shahdan, Intan Azura Long, Kamariah Haron, Ummi Aqilah
author_sort	Mukhtar, Nor Izzah
title	Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans
title_short	Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans
title_full	Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans
title_fullStr	Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans
title_full_unstemmed	Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans
title_sort	application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans
publishDate	2019
url	http://irep.iium.edu.my/75103/ http://irep.iium.edu.my/75103/ http://irep.iium.edu.my/75103/1/MJAS%202019.pdf http://irep.iium.edu.my/75103/3/letter%20from%20publisher.pdf http://irep.iium.edu.my/75103/9/75103_Application%20of%20direct%20fluorescence-based%20live%20or%20dead%20staining%20for%20assessment%20of%20antifungal%20activity%20of%20coconut%20oil%20against%20candida%20albicans_Scopus.pdf
first_indexed	2023-09-18T21:46:17Z
last_indexed	2023-09-18T21:46:17Z
_version_	1777413471373099008

Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans

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