Human amniotic membrane as a matrix for endothelial differentiation of VEGF-treated dental stem cells

Introduction—Endothelial cells cover the surface of the capillary wall and literature review has cemented its angiogenic roles in wound healing and tissue regeneration. However, the angiogenic in vitro models available are inadequate to understand the endothelial differentiation process. Methods—A...

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Main Authors:	Md Hashim, Siti Nurnasihah, Yusof, Muhammad Fuad Hilmi, Zahari, Wafa’, Chandra, Hamshawagini, Ahmad Amin Noordin, Khairul Bariah, Kannan, Thirumulu Ponnuraj, Sheikh Abdul Hamid, Suzina, Mokhtar@Makhtar, Khairani Idah, Ahmad, Azlina
Format:	Article
Language:	English English
Published:	Springer 2019
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/75120/ http://irep.iium.edu.my/75120/ http://irep.iium.edu.my/75120/ http://irep.iium.edu.my/75120/1/Siti%20Nurnasihah%20et%20al%202019-BMES.pdf http://irep.iium.edu.my/75120/7/75120_Indexed%20in%20Scopus.pdf

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recordtype	eprints
spelling	iium-751202019-12-04T09:47:21Z http://irep.iium.edu.my/75120/ Human amniotic membrane as a matrix for endothelial differentiation of VEGF-treated dental stem cells Md Hashim, Siti Nurnasihah Yusof, Muhammad Fuad Hilmi Zahari, Wafa’ Chandra, Hamshawagini Ahmad Amin Noordin, Khairul Bariah Kannan, Thirumulu Ponnuraj Sheikh Abdul Hamid, Suzina Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina RK Dentistry Introduction—Endothelial cells cover the surface of the capillary wall and literature review has cemented its angiogenic roles in wound healing and tissue regeneration. However, the angiogenic in vitro models available are inadequate to understand the endothelial differentiation process. Methods—A construct was made using human amniotic membrane (HAM) as a matrix to assist the dental stem cells to differentiate into endothelial-like cells. This study aimed to assess the biological interaction between stem cells from human exfoliated deciduous teeth (SHED) and the stromal side (SS) of the glycerol-preserved HAM in angiogenic induced environment media using VEGF. The changes were evaluated through cell morphology, migration, as well as gene expression level. Results—There were morphological changes observed in SHED in angiogenic-induced media. SHED appeared to be differentiated from fibroblast-like cells to a new structure, mimicking endothelial-like structure through microscopy analysis. Besides, the cross-section of the construct revealed that the cells seeded on the matrix were able to maintain its monolayer structure at day 1, 7 and 14 but infiltrated into the HAM at day 21, suggesting cell migration. The cells were also able to maintain its stemness (Nestin, Nanog and CD29) and at the same time express the angiogenic markers (IL-8, VEGF and MMP-2). Conclusion—HAM promotes SHED proliferation, migration and has the potential as a differentiating matrix for endothelial-like cells. Springer 2019-09-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/75120/1/Siti%20Nurnasihah%20et%20al%202019-BMES.pdf application/pdf en http://irep.iium.edu.my/75120/7/75120_Indexed%20in%20Scopus.pdf Md Hashim, Siti Nurnasihah and Yusof, Muhammad Fuad Hilmi and Zahari, Wafa’ and Chandra, Hamshawagini and Ahmad Amin Noordin, Khairul Bariah and Kannan, Thirumulu Ponnuraj and Sheikh Abdul Hamid, Suzina and Mokhtar@Makhtar, Khairani Idah and Ahmad, Azlina (2019) Human amniotic membrane as a matrix for endothelial differentiation of VEGF-treated dental stem cells. Cellular and Molecular Bioengineering, Vol. 12 (6). pp. 599-613. ISSN 1865-5025 E-ISSN 1865-5033 https://link.springer.com https://doi.org/10.1007/s12195-019-00596-x
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RK Dentistry
spellingShingle	RK Dentistry Md Hashim, Siti Nurnasihah Yusof, Muhammad Fuad Hilmi Zahari, Wafa’ Chandra, Hamshawagini Ahmad Amin Noordin, Khairul Bariah Kannan, Thirumulu Ponnuraj Sheikh Abdul Hamid, Suzina Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina Human amniotic membrane as a matrix for endothelial differentiation of VEGF-treated dental stem cells
description	Introduction—Endothelial cells cover the surface of the capillary wall and literature review has cemented its angiogenic roles in wound healing and tissue regeneration. However, the angiogenic in vitro models available are inadequate to understand the endothelial differentiation process. Methods—A construct was made using human amniotic membrane (HAM) as a matrix to assist the dental stem cells to differentiate into endothelial-like cells. This study aimed to assess the biological interaction between stem cells from human exfoliated deciduous teeth (SHED) and the stromal side (SS) of the glycerol-preserved HAM in angiogenic induced environment media using VEGF. The changes were evaluated through cell morphology, migration, as well as gene expression level. Results—There were morphological changes observed in SHED in angiogenic-induced media. SHED appeared to be differentiated from fibroblast-like cells to a new structure, mimicking endothelial-like structure through microscopy analysis. Besides, the cross-section of the construct revealed that the cells seeded on the matrix were able to maintain its monolayer structure at day 1, 7 and 14 but infiltrated into the HAM at day 21, suggesting cell migration. The cells were also able to maintain its stemness (Nestin, Nanog and CD29) and at the same time express the angiogenic markers (IL-8, VEGF and MMP-2). Conclusion—HAM promotes SHED proliferation, migration and has the potential as a differentiating matrix for endothelial-like cells.
format	Article
author	Md Hashim, Siti Nurnasihah Yusof, Muhammad Fuad Hilmi Zahari, Wafa’ Chandra, Hamshawagini Ahmad Amin Noordin, Khairul Bariah Kannan, Thirumulu Ponnuraj Sheikh Abdul Hamid, Suzina Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina
author_facet	Md Hashim, Siti Nurnasihah Yusof, Muhammad Fuad Hilmi Zahari, Wafa’ Chandra, Hamshawagini Ahmad Amin Noordin, Khairul Bariah Kannan, Thirumulu Ponnuraj Sheikh Abdul Hamid, Suzina Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina
author_sort	Md Hashim, Siti Nurnasihah
title	Human amniotic membrane as a matrix for endothelial differentiation of VEGF-treated dental stem cells
title_short	Human amniotic membrane as a matrix for endothelial differentiation of VEGF-treated dental stem cells
title_full	Human amniotic membrane as a matrix for endothelial differentiation of VEGF-treated dental stem cells
title_fullStr	Human amniotic membrane as a matrix for endothelial differentiation of VEGF-treated dental stem cells
title_full_unstemmed	Human amniotic membrane as a matrix for endothelial differentiation of VEGF-treated dental stem cells
title_sort	human amniotic membrane as a matrix for endothelial differentiation of vegf-treated dental stem cells
publisher	Springer
publishDate	2019
url	http://irep.iium.edu.my/75120/ http://irep.iium.edu.my/75120/ http://irep.iium.edu.my/75120/ http://irep.iium.edu.my/75120/1/Siti%20Nurnasihah%20et%20al%202019-BMES.pdf http://irep.iium.edu.my/75120/7/75120_Indexed%20in%20Scopus.pdf
first_indexed	2023-09-18T21:46:18Z
last_indexed	2023-09-18T21:46:18Z
_version_	1777413472400703488

Human amniotic membrane as a matrix for endothelial differentiation of VEGF-treated dental stem cells

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