The effects of Aquilaria malaccensis leaves aqueous extract on sperm of sprague dawley rats towards early embryogenesis.

Introduction: Oxidative stress induced by excessive and unopposed levels of reactive oxygen species in male reproductive system results in impaired sperm quality, fertilization capacity and poor embryo development. Our goal is to assess the potential effects of Aquilaria malaccensis (AM) leaves, a...

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Main Authors:	Ismail, Faridah, Abdul Wahab, Azantee Yazmie, Md Isa, Muhammad Lokman, Muhammad, Hussin, Raja Ismail, Raja Arif Shah, Abdul Razak, Redzuan Nul Hakim
Format:	Article
Language:	English English
Published:	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2019
Subjects:	Q Science (General) R Medicine (General) RG Gynecology and obstetrics RM Therapeutics. Pharmacology RV Botanic, Thomsonian, and eclectic medicine SD Forestry
Online Access:	http://irep.iium.edu.my/75301/ http://irep.iium.edu.my/75301/ http://irep.iium.edu.my/75301/13/75301%20The%20Effects%20of%20Aquilaria%20malaccensis%20Leaves%20Aqueous.pdf http://irep.iium.edu.my/75301/14/75301%20The%20Effects%20of%20Aquilaria%20malaccensis%20Leaves%20Aqueous%20SCOPUS.pdf

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spelling	iium-753012020-01-09T06:44:51Z http://irep.iium.edu.my/75301/ The effects of Aquilaria malaccensis leaves aqueous extract on sperm of sprague dawley rats towards early embryogenesis. Ismail, Faridah Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Muhammad, Hussin Raja Ismail, Raja Arif Shah Abdul Razak, Redzuan Nul Hakim Q Science (General) R Medicine (General) RG Gynecology and obstetrics RM Therapeutics. Pharmacology RV Botanic, Thomsonian, and eclectic medicine SD Forestry Introduction: Oxidative stress induced by excessive and unopposed levels of reactive oxygen species in male reproductive system results in impaired sperm quality, fertilization capacity and poor embryo development. Our goal is to assess the potential effects of Aquilaria malaccensis (AM) leaves, a plant with strong antioxidant property on early embryo development in vitro and embryo quality following fertilization with cyclophosphamide (CP) exposed rat sperm. Materials and Methods: Twenty four male Sprague Dawley rats were allocated into eight groups of three rats (n = 3): control, CP only (200 mg/kg), AM only (100 mg/kg, 300 mg/kg and 500 mg/kg) and CP + AM (100 mg/kg, 300 mg/kg and 500 mg/kg). Animals were sacrificed after 63 days of treatment and sperm from caudal epididymis were taken for in vitro fertilization (IVF) with oocytes from untreated female. Fertilization, embryo division and embryo morphology were examined at 8 and 48 hours post insemination and compared between groups. Statistical evaluations were performed using Chi-Square test and Fisher’s exact test and p-value<0.05 was considered significant. Results: Administration of AM leave extract at 100 mg/kg/day to normal rats and CP-exposed rats has significantly increased (p<0.05) the fertilization rate, early cleavage rate and embryo quality when compared to CP only treated group. However, other groups showed no significant differences. Conclusion: Overall, the present results indicate the potential of AM leave extract supplementation to improve the fertility and early embryo development in male rat exposed to CP by inhibiting the oxidative processes and scavenging free radicals. Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2019-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/75301/13/75301%20The%20Effects%20of%20Aquilaria%20malaccensis%20Leaves%20Aqueous.pdf application/pdf en http://irep.iium.edu.my/75301/14/75301%20The%20Effects%20of%20Aquilaria%20malaccensis%20Leaves%20Aqueous%20SCOPUS.pdf Ismail, Faridah and Abdul Wahab, Azantee Yazmie and Md Isa, Muhammad Lokman and Muhammad, Hussin and Raja Ismail, Raja Arif Shah and Abdul Razak, Redzuan Nul Hakim (2019) The effects of Aquilaria malaccensis leaves aqueous extract on sperm of sprague dawley rats towards early embryogenesis. International Medical Journal of Malaysia, 18 (2). pp. 59-68. ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/volume_18_no_2/Pages-from-IMJMVol18No2-11.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General) R Medicine (General) RG Gynecology and obstetrics RM Therapeutics. Pharmacology RV Botanic, Thomsonian, and eclectic medicine SD Forestry
spellingShingle	Q Science (General) R Medicine (General) RG Gynecology and obstetrics RM Therapeutics. Pharmacology RV Botanic, Thomsonian, and eclectic medicine SD Forestry Ismail, Faridah Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Muhammad, Hussin Raja Ismail, Raja Arif Shah Abdul Razak, Redzuan Nul Hakim The effects of Aquilaria malaccensis leaves aqueous extract on sperm of sprague dawley rats towards early embryogenesis.
description	Introduction: Oxidative stress induced by excessive and unopposed levels of reactive oxygen species in male reproductive system results in impaired sperm quality, fertilization capacity and poor embryo development. Our goal is to assess the potential effects of Aquilaria malaccensis (AM) leaves, a plant with strong antioxidant property on early embryo development in vitro and embryo quality following fertilization with cyclophosphamide (CP) exposed rat sperm. Materials and Methods: Twenty four male Sprague Dawley rats were allocated into eight groups of three rats (n = 3): control, CP only (200 mg/kg), AM only (100 mg/kg, 300 mg/kg and 500 mg/kg) and CP + AM (100 mg/kg, 300 mg/kg and 500 mg/kg). Animals were sacrificed after 63 days of treatment and sperm from caudal epididymis were taken for in vitro fertilization (IVF) with oocytes from untreated female. Fertilization, embryo division and embryo morphology were examined at 8 and 48 hours post insemination and compared between groups. Statistical evaluations were performed using Chi-Square test and Fisher’s exact test and p-value<0.05 was considered significant. Results: Administration of AM leave extract at 100 mg/kg/day to normal rats and CP-exposed rats has significantly increased (p<0.05) the fertilization rate, early cleavage rate and embryo quality when compared to CP only treated group. However, other groups showed no significant differences. Conclusion: Overall, the present results indicate the potential of AM leave extract supplementation to improve the fertility and early embryo development in male rat exposed to CP by inhibiting the oxidative processes and scavenging free radicals.
format	Article
author	Ismail, Faridah Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Muhammad, Hussin Raja Ismail, Raja Arif Shah Abdul Razak, Redzuan Nul Hakim
author_facet	Ismail, Faridah Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Muhammad, Hussin Raja Ismail, Raja Arif Shah Abdul Razak, Redzuan Nul Hakim
author_sort	Ismail, Faridah
title	The effects of Aquilaria malaccensis leaves aqueous extract on sperm of sprague dawley rats towards early embryogenesis.
title_short	The effects of Aquilaria malaccensis leaves aqueous extract on sperm of sprague dawley rats towards early embryogenesis.
title_full	The effects of Aquilaria malaccensis leaves aqueous extract on sperm of sprague dawley rats towards early embryogenesis.
title_fullStr	The effects of Aquilaria malaccensis leaves aqueous extract on sperm of sprague dawley rats towards early embryogenesis.
title_full_unstemmed	The effects of Aquilaria malaccensis leaves aqueous extract on sperm of sprague dawley rats towards early embryogenesis.
title_sort	effects of aquilaria malaccensis leaves aqueous extract on sperm of sprague dawley rats towards early embryogenesis.
publisher	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
publishDate	2019
url	http://irep.iium.edu.my/75301/ http://irep.iium.edu.my/75301/ http://irep.iium.edu.my/75301/13/75301%20The%20Effects%20of%20Aquilaria%20malaccensis%20Leaves%20Aqueous.pdf http://irep.iium.edu.my/75301/14/75301%20The%20Effects%20of%20Aquilaria%20malaccensis%20Leaves%20Aqueous%20SCOPUS.pdf
first_indexed	2023-09-18T21:46:33Z
last_indexed	2023-09-18T21:46:33Z
_version_	1777413488447062016

The effects of Aquilaria malaccensis leaves aqueous extract on sperm of sprague dawley rats towards early embryogenesis.

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