An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria

The development of treatment strategies for periodontitis that maximise the effectiveness of antibiotics is highly desirable. Azithromycin is proving to be an effective antibiotic for treatment of refractory periodontitis which works by binding to the outer membrane of Gram-negative bacteria and sub...

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Main Authors:	Husain, Juzaily, Griffiths, Gareth S., Rawlinson, Andrew, Stafford, Graham, Douglas, zC.W. Ian
Format:	Conference or Workshop Item
Language:	English English
Published:	Elsevier 2019
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/75318/ http://irep.iium.edu.my/75318/ http://irep.iium.edu.my/75318/ http://irep.iium.edu.my/75318/7/75318%20An%20investigation%20into%20the%20synergistic.pdf http://irep.iium.edu.my/75318/8/75318%20An%20investigation%20into%20the%20synergistic%20SCOPUS.pdf

id	iium-75318
recordtype	eprints
spelling	iium-753182019-11-15T00:52:33Z http://irep.iium.edu.my/75318/ An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria Husain, Juzaily Griffiths, Gareth S. Rawlinson, Andrew Stafford, Graham Douglas, zC.W. Ian RK Dentistry The development of treatment strategies for periodontitis that maximise the effectiveness of antibiotics is highly desirable. Azithromycin is proving to be an effective antibiotic for treatment of refractory periodontitis which works by binding to the outer membrane of Gram-negative bacteria and subsequently inhibits protein synthesis. Lactoferrin is a membrane-active host antimicrobial protein and so the objective of this study was to determine whether the effect of azithromycin (AZM) against example periodontopathogens (Porphyromonas gingivalis and Tannerella forsythia) could be potentiated by lactoferrin. Two strains of P.gingivalis and T. forsythia were exposed to lactoferrin (LF; up to 10 mg/ml) and AZM (up to 5 μg/ml) for 0 – 72 h. The MICs for AZM were established using E-Test strips and by agar diffusion. Susceptibility to LF and LF + AZM was evaluated using diffusion assays, with and without iron depletion. The range of MIC values of AZM for P. gingivalis strains and T. forsythia was 0.16 - 0.63 μg/ml and 0.50 - 0.63 μg/ml, respectively. However, no inhibition was observed with iron saturated lactoferrin at any concentration or under iron depletion conditions nor was any effect observed on the AZM MIC by its presence. P. gingivalis and T. forsythia were inhibited by AZM but were not affected by Lf and there was no synergism between AZM and Lf. Elsevier 2019 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/75318/7/75318%20An%20investigation%20into%20the%20synergistic.pdf application/pdf en http://irep.iium.edu.my/75318/8/75318%20An%20investigation%20into%20the%20synergistic%20SCOPUS.pdf Husain, Juzaily and Griffiths, Gareth S. and Rawlinson, Andrew and Stafford, Graham and Douglas, zC.W. Ian (2019) An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria. In: Conference on BioMedical and Advanced Materials (Bio-CAM 2017), 28th-29th November 2017, Pulau Langkawi, Kedah. https://www.sciencedirect.com/science/article/pii/S2214785319314786 10.1016/j.matpr.2019.06.129
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RK Dentistry
spellingShingle	RK Dentistry Husain, Juzaily Griffiths, Gareth S. Rawlinson, Andrew Stafford, Graham Douglas, zC.W. Ian An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria
description	The development of treatment strategies for periodontitis that maximise the effectiveness of antibiotics is highly desirable. Azithromycin is proving to be an effective antibiotic for treatment of refractory periodontitis which works by binding to the outer membrane of Gram-negative bacteria and subsequently inhibits protein synthesis. Lactoferrin is a membrane-active host antimicrobial protein and so the objective of this study was to determine whether the effect of azithromycin (AZM) against example periodontopathogens (Porphyromonas gingivalis and Tannerella forsythia) could be potentiated by lactoferrin. Two strains of P.gingivalis and T. forsythia were exposed to lactoferrin (LF; up to 10 mg/ml) and AZM (up to 5 μg/ml) for 0 – 72 h. The MICs for AZM were established using E-Test strips and by agar diffusion. Susceptibility to LF and LF + AZM was evaluated using diffusion assays, with and without iron depletion. The range of MIC values of AZM for P. gingivalis strains and T. forsythia was 0.16 - 0.63 μg/ml and 0.50 - 0.63 μg/ml, respectively. However, no inhibition was observed with iron saturated lactoferrin at any concentration or under iron depletion conditions nor was any effect observed on the AZM MIC by its presence. P. gingivalis and T. forsythia were inhibited by AZM but were not affected by Lf and there was no synergism between AZM and Lf.
format	Conference or Workshop Item
author	Husain, Juzaily Griffiths, Gareth S. Rawlinson, Andrew Stafford, Graham Douglas, zC.W. Ian
author_facet	Husain, Juzaily Griffiths, Gareth S. Rawlinson, Andrew Stafford, Graham Douglas, zC.W. Ian
author_sort	Husain, Juzaily
title	An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria
title_short	An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria
title_full	An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria
title_fullStr	An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria
title_full_unstemmed	An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria
title_sort	investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria
publisher	Elsevier
publishDate	2019
url	http://irep.iium.edu.my/75318/ http://irep.iium.edu.my/75318/ http://irep.iium.edu.my/75318/ http://irep.iium.edu.my/75318/7/75318%20An%20investigation%20into%20the%20synergistic.pdf http://irep.iium.edu.my/75318/8/75318%20An%20investigation%20into%20the%20synergistic%20SCOPUS.pdf
first_indexed	2023-09-18T21:46:35Z
last_indexed	2023-09-18T21:46:35Z
_version_	1777413490039848960

An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria

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