Analysis on the inhibition of periodontal pathogens and its virulence genes expression by virgin coconut oil using quantitative real-time reverse transcription-PCR (qRT-PCR)

Introduction: Dental caries has remained a major oral health problem worldwide. Streptococcus mutans is considered as a vital cariogenic agent in the oral cavity. It can synthesise soluble and insoluble glucans from sucrose by glucosyltransferases enzymes and develops stable biofilm on the tooth sur...

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Main Authors:	Abllah, Zurainie, Omar, Muhammad Nor, Arief Ichwan, Solachuddin Jauhari, Shahdan, Intan Azura, Jalaludin, Anil Azura, Haron, Ummi Aqilah, Mukhtar, Nor Izzah
Format:	Monograph
Language:	English
Published:	2019
Subjects:	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
Online Access:	http://irep.iium.edu.my/75362/ http://irep.iium.edu.my/75362/1/Profile%20of%20Final%20Report_FRGS%20DRZURAINIE.pdf

id	iium-75362
recordtype	eprints
spelling	iium-753622019-10-15T08:30:10Z http://irep.iium.edu.my/75362/ Analysis on the inhibition of periodontal pathogens and its virulence genes expression by virgin coconut oil using quantitative real-time reverse transcription-PCR (qRT-PCR) Abllah, Zurainie Omar, Muhammad Nor Arief Ichwan, Solachuddin Jauhari Shahdan, Intan Azura Jalaludin, Anil Azura Haron, Ummi Aqilah Mukhtar, Nor Izzah RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works Introduction: Dental caries has remained a major oral health problem worldwide. Streptococcus mutans is considered as a vital cariogenic agent in the oral cavity. It can synthesise soluble and insoluble glucans from sucrose by glucosyltransferases enzymes and develops stable biofilm on the tooth surface. In the previous study, the fatty acids profile of activated virgin coconut oil (AVCO) have been described, and it includes the excellent antimicrobial activity of the oil. The current study aims to evaluate the antibiofilm effect of AVCO against the Streptococcus mutans ATCC 2157 biofilm in vitro. Materials and method: The minimum biofilm inhibition concentration (MBIC) and the minimum biofilm eradication concentration (MBEC) for antibiofilm activity were determined by serial dilution method, and biofilm thickness on S. mutans was quantified by confocal laser scanning microscopy (CLSM). Results: At the concentra-tion of 15.63 mg/ml of AVCO, it successfully inhibits the development of S. mutans ATCC 21575 biofilm and AVCO (62.52 mg/ml) eradicate biofilm that was formed by the bacteria. The z-stack images obtained from CLSM allows the construction of 3-D biofilm structure and a significant difference in the thickness of S. mutans ATCC 21575 biofilm pre and post-treatment with AVCO were observed. Conclusion: AVCO showed good potential as anticaries where it possesses the ability to inhibits and eradicate S. mutans ATCC 21575 biofilm. A future study to evaluates the interaction of individuals fatty acids present in AVCO against proteins that relate to biofilm formation of S. mutans can be performed utilising the molecular docking tools. 2019-07-31 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/75362/1/Profile%20of%20Final%20Report_FRGS%20DRZURAINIE.pdf Abllah, Zurainie and Omar, Muhammad Nor and Arief Ichwan, Solachuddin Jauhari and Shahdan, Intan Azura and Jalaludin, Anil Azura and Haron, Ummi Aqilah and Mukhtar, Nor Izzah (2019) Analysis on the inhibition of periodontal pathogens and its virulence genes expression by virgin coconut oil using quantitative real-time reverse transcription-PCR (qRT-PCR). Project Report. UNSPECIFIED. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
spellingShingle	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works Abllah, Zurainie Omar, Muhammad Nor Arief Ichwan, Solachuddin Jauhari Shahdan, Intan Azura Jalaludin, Anil Azura Haron, Ummi Aqilah Mukhtar, Nor Izzah Analysis on the inhibition of periodontal pathogens and its virulence genes expression by virgin coconut oil using quantitative real-time reverse transcription-PCR (qRT-PCR)
description	Introduction: Dental caries has remained a major oral health problem worldwide. Streptococcus mutans is considered as a vital cariogenic agent in the oral cavity. It can synthesise soluble and insoluble glucans from sucrose by glucosyltransferases enzymes and develops stable biofilm on the tooth surface. In the previous study, the fatty acids profile of activated virgin coconut oil (AVCO) have been described, and it includes the excellent antimicrobial activity of the oil. The current study aims to evaluate the antibiofilm effect of AVCO against the Streptococcus mutans ATCC 2157 biofilm in vitro. Materials and method: The minimum biofilm inhibition concentration (MBIC) and the minimum biofilm eradication concentration (MBEC) for antibiofilm activity were determined by serial dilution method, and biofilm thickness on S. mutans was quantified by confocal laser scanning microscopy (CLSM). Results: At the concentra-tion of 15.63 mg/ml of AVCO, it successfully inhibits the development of S. mutans ATCC 21575 biofilm and AVCO (62.52 mg/ml) eradicate biofilm that was formed by the bacteria. The z-stack images obtained from CLSM allows the construction of 3-D biofilm structure and a significant difference in the thickness of S. mutans ATCC 21575 biofilm pre and post-treatment with AVCO were observed. Conclusion: AVCO showed good potential as anticaries where it possesses the ability to inhibits and eradicate S. mutans ATCC 21575 biofilm. A future study to evaluates the interaction of individuals fatty acids present in AVCO against proteins that relate to biofilm formation of S. mutans can be performed utilising the molecular docking tools.
format	Monograph
author	Abllah, Zurainie Omar, Muhammad Nor Arief Ichwan, Solachuddin Jauhari Shahdan, Intan Azura Jalaludin, Anil Azura Haron, Ummi Aqilah Mukhtar, Nor Izzah
author_facet	Abllah, Zurainie Omar, Muhammad Nor Arief Ichwan, Solachuddin Jauhari Shahdan, Intan Azura Jalaludin, Anil Azura Haron, Ummi Aqilah Mukhtar, Nor Izzah
author_sort	Abllah, Zurainie
title	Analysis on the inhibition of periodontal pathogens and its virulence genes expression by virgin coconut oil using quantitative real-time reverse transcription-PCR (qRT-PCR)
title_short	Analysis on the inhibition of periodontal pathogens and its virulence genes expression by virgin coconut oil using quantitative real-time reverse transcription-PCR (qRT-PCR)
title_full	Analysis on the inhibition of periodontal pathogens and its virulence genes expression by virgin coconut oil using quantitative real-time reverse transcription-PCR (qRT-PCR)
title_fullStr	Analysis on the inhibition of periodontal pathogens and its virulence genes expression by virgin coconut oil using quantitative real-time reverse transcription-PCR (qRT-PCR)
title_full_unstemmed	Analysis on the inhibition of periodontal pathogens and its virulence genes expression by virgin coconut oil using quantitative real-time reverse transcription-PCR (qRT-PCR)
title_sort	analysis on the inhibition of periodontal pathogens and its virulence genes expression by virgin coconut oil using quantitative real-time reverse transcription-pcr (qrt-pcr)
publishDate	2019
url	http://irep.iium.edu.my/75362/ http://irep.iium.edu.my/75362/1/Profile%20of%20Final%20Report_FRGS%20DRZURAINIE.pdf
first_indexed	2023-09-18T21:46:37Z
last_indexed	2023-09-18T21:46:37Z
_version_	1777413493056602112

Analysis on the inhibition of periodontal pathogens and its virulence genes expression by virgin coconut oil using quantitative real-time reverse transcription-PCR (qRT-PCR)

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