Genome comparison of Actinomyces naeslundii with 25 known probiotics

Introduction: Actinomyces naeslundii is an oral microbiome that has been suggested to involve in actinomycosis particularly in immunocompromised patients. However, studies have also shown that the bacterium can inhibit the colonisation of Candida albicans and cariogenic bacterium, Streptococcus muta...

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Main Authors:	Othman, Noratikah, Arzmi, Mohd Hafiz, Mohamad, Noor Afifah hanin
Format:	Conference or Workshop Item
Language:	English English
Published:	2019
Subjects:	QR Microbiology RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
Online Access:	http://irep.iium.edu.my/75373/ http://irep.iium.edu.my/75373/1/Poster%20MRS%202019.pdf http://irep.iium.edu.my/75373/13/75373%20Abstract.pdf

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spelling	iium-753732019-12-17T00:44:54Z http://irep.iium.edu.my/75373/ Genome comparison of Actinomyces naeslundii with 25 known probiotics Othman, Noratikah Arzmi, Mohd Hafiz Mohamad, Noor Afifah hanin QR Microbiology RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works Introduction: Actinomyces naeslundii is an oral microbiome that has been suggested to involve in actinomycosis particularly in immunocompromised patients. However, studies have also shown that the bacterium can inhibit the colonisation of Candida albicans and cariogenic bacterium, Streptococcus mutans. Even though various studies have been conducted to characterise A. naeslundii, however, the role of the bacterium in the oral cavity either to induce pathogenicity or to improve oral health remain unclear. The objective of this study is to determine the role of A. naeslundii in the oral cavity using bioinformatic tools with the hypothesis that A. naeslundii possesses genome similarity to oral probiotics. Materials and method: The study was conducted by data mining of the genomic sequences of A. naeslundii NCTC10301 from GenBank. Following that, genomic comparison was performed with 25 species of well-known probiotics. MAUVE version 2.3.1 was used to find similarities between A. naeslundii with the known probiotics through identification of potential relevance probiotic properties in A. naeslundii. Phylogenetic tree was constructed using PHYLIP package to identify evolutionary relationship of A. naeslundii with the known probiotics. The 16s rRNA sequences of A. naeslundii and known probiotics were harvested from GenBank using BLAST. Finally, the functions of all A. naeslundii genes that shared similarity with known probiotics were identified using INTERPRO. Result: Genome comparison analysis of the present study showed that A. naeslundii genome exhibited high similarity to Bifidobacterium species such as B. animalis subsp. lactis, B. bifidum, B. longum and B. breve. In addition, phylogenetic tree analysis showed that Bacillus species, B. subtilis and B. cereus , appeared to clade together with A. naeslundii , with bootstrap value of 98%. Conclusion: A. naeslundii had high similarity with probiotic Bifidobacterium species thus supported the hypothesis of the present study that A. naeslundii possesses genome similarity to oral probiotic. 2019 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/75373/1/Poster%20MRS%202019.pdf application/pdf en http://irep.iium.edu.my/75373/13/75373%20Abstract.pdf Othman, Noratikah and Arzmi, Mohd Hafiz and Mohamad, Noor Afifah hanin (2019) Genome comparison of Actinomyces naeslundii with 25 known probiotics. In: Medical Research Symposium 2019, 9th Oct 2019, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
spellingShingle	QR Microbiology RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works Othman, Noratikah Arzmi, Mohd Hafiz Mohamad, Noor Afifah hanin Genome comparison of Actinomyces naeslundii with 25 known probiotics
description	Introduction: Actinomyces naeslundii is an oral microbiome that has been suggested to involve in actinomycosis particularly in immunocompromised patients. However, studies have also shown that the bacterium can inhibit the colonisation of Candida albicans and cariogenic bacterium, Streptococcus mutans. Even though various studies have been conducted to characterise A. naeslundii, however, the role of the bacterium in the oral cavity either to induce pathogenicity or to improve oral health remain unclear. The objective of this study is to determine the role of A. naeslundii in the oral cavity using bioinformatic tools with the hypothesis that A. naeslundii possesses genome similarity to oral probiotics. Materials and method: The study was conducted by data mining of the genomic sequences of A. naeslundii NCTC10301 from GenBank. Following that, genomic comparison was performed with 25 species of well-known probiotics. MAUVE version 2.3.1 was used to find similarities between A. naeslundii with the known probiotics through identification of potential relevance probiotic properties in A. naeslundii. Phylogenetic tree was constructed using PHYLIP package to identify evolutionary relationship of A. naeslundii with the known probiotics. The 16s rRNA sequences of A. naeslundii and known probiotics were harvested from GenBank using BLAST. Finally, the functions of all A. naeslundii genes that shared similarity with known probiotics were identified using INTERPRO. Result: Genome comparison analysis of the present study showed that A. naeslundii genome exhibited high similarity to Bifidobacterium species such as B. animalis subsp. lactis, B. bifidum, B. longum and B. breve. In addition, phylogenetic tree analysis showed that Bacillus species, B. subtilis and B. cereus , appeared to clade together with A. naeslundii , with bootstrap value of 98%. Conclusion: A. naeslundii had high similarity with probiotic Bifidobacterium species thus supported the hypothesis of the present study that A. naeslundii possesses genome similarity to oral probiotic.
format	Conference or Workshop Item
author	Othman, Noratikah Arzmi, Mohd Hafiz Mohamad, Noor Afifah hanin
author_facet	Othman, Noratikah Arzmi, Mohd Hafiz Mohamad, Noor Afifah hanin
author_sort	Othman, Noratikah
title	Genome comparison of Actinomyces naeslundii with 25 known probiotics
title_short	Genome comparison of Actinomyces naeslundii with 25 known probiotics
title_full	Genome comparison of Actinomyces naeslundii with 25 known probiotics
title_fullStr	Genome comparison of Actinomyces naeslundii with 25 known probiotics
title_full_unstemmed	Genome comparison of Actinomyces naeslundii with 25 known probiotics
title_sort	genome comparison of actinomyces naeslundii with 25 known probiotics
publishDate	2019
url	http://irep.iium.edu.my/75373/ http://irep.iium.edu.my/75373/1/Poster%20MRS%202019.pdf http://irep.iium.edu.my/75373/13/75373%20Abstract.pdf
first_indexed	2023-09-18T21:46:38Z
last_indexed	2023-09-18T21:46:38Z
_version_	1777413493968863232

Genome comparison of Actinomyces naeslundii with 25 known probiotics

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