Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia

Vibrio infections are common among marine fish and lead to serious problems in the aquaculture sector. This study reports a recent occurrence of Vibrio species (spp.) isolated from cultured groupers in Peninsular Malaysia using the gyrB and pyrH genes. A total of 147 Vibrio strains were successfully...

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Main Authors:	Zulkipli, Nor Amalina, Zulperi, Dzarifah, Azmai Amal, Mohammad Noor, Yusof, Mohd Termizi, Saad, Mohd Zamri, Al-saari, Nurhidayu, Tanaka, Mami, Mino, Sayaka, Sawabe, Tomoo, Md Yasin, Ina‐Salwany
Format:	Article
Language:	English English
Published:	Wiley Online Library 2019
Subjects:	QR Microbiology SH Aquaculture. Fisheries. Angling
Online Access:	http://irep.iium.edu.my/75657/ http://irep.iium.edu.my/75657/ http://irep.iium.edu.my/75657/ http://irep.iium.edu.my/75657/19/75657%20Recent%20update%20on%20the%20prevalence%20of%20Vibrio.pdf http://irep.iium.edu.my/75657/7/75657_Recent%20update%20on%20the%20prevalence%20of%20Vibrio%20species%20among%20cultured%20grouper%20in%20Peninsular%20Malaysia_Scopus.pdf

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spelling	iium-756572020-01-07T03:42:46Z http://irep.iium.edu.my/75657/ Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia Zulkipli, Nor Amalina Zulperi, Dzarifah Azmai Amal, Mohammad Noor Yusof, Mohd Termizi Saad, Mohd Zamri Al-saari, Nurhidayu Tanaka, Mami Mino, Sayaka Sawabe, Tomoo Md Yasin, Ina‐Salwany QR Microbiology SH Aquaculture. Fisheries. Angling Vibrio infections are common among marine fish and lead to serious problems in the aquaculture sector. This study reports a recent occurrence of Vibrio species (spp.) isolated from cultured groupers in Peninsular Malaysia using the gyrB and pyrH genes. A total of 147 Vibrio strains were successfully isolated from 77 (64%) groupers using culture method and subjected to gyrB and pyrH sequencing for species identifica‐ tion and confirmation. Results showed that 89% of Vibrio strains were identified and clustered to six groups of Vibrio spp., while 11% were not clustered to any Vibrio spp. using the gyrB sequences. Meanwhile, by analysis of the pyrH sequences all the 147 Vibrio strains (100%) were successfully identified and clustered into 11 groups of Vibrio spp., including the gyrB non‐identified strains. The pyrH gene provides a better resolution for identification of Vibrio spp. compared with the gyrB gene. Thus, the pyrH gene was more suitable for a rapid determination of Vibrio spp. distribution in Peninsular Malaysia. Using the pyrH gene, our study found higher prevalence of Vibrio vulnificus (33%), V. alginolyticus (24%) and V. parahaemolyticus (22%), followed by V. rotiferianus (5%), V. harveyi (3%), V. tubiashii (2%), V. campbellii (2%), V. ponticus (1%), V. diabolicus (1%), V. owensii (1%) and others Vibrio sp. (7%). Thus, the results of this study revealed that the occurrence of pathogenic vibrios among grouper fish is still high in Malaysian aquaculture. In addition, the pyrH gene was proved as a suitable marker for rapid identification of Vibrio species compared with the gyrB gene. Wiley Online Library 2019-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/75657/19/75657%20Recent%20update%20on%20the%20prevalence%20of%20Vibrio.pdf application/pdf en http://irep.iium.edu.my/75657/7/75657_Recent%20update%20on%20the%20prevalence%20of%20Vibrio%20species%20among%20cultured%20grouper%20in%20Peninsular%20Malaysia_Scopus.pdf Zulkipli, Nor Amalina and Zulperi, Dzarifah and Azmai Amal, Mohammad Noor and Yusof, Mohd Termizi and Saad, Mohd Zamri and Al-saari, Nurhidayu and Tanaka, Mami and Mino, Sayaka and Sawabe, Tomoo and Md Yasin, Ina‐Salwany (2019) Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia. Aquaculture Research, 50 (11). pp. 3202-3210. E-ISSN 1365-2109 https://onlinelibrary.wiley.com/doi/abs/10.1111/are.14275 10.1111/are.14275
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology SH Aquaculture. Fisheries. Angling
spellingShingle	QR Microbiology SH Aquaculture. Fisheries. Angling Zulkipli, Nor Amalina Zulperi, Dzarifah Azmai Amal, Mohammad Noor Yusof, Mohd Termizi Saad, Mohd Zamri Al-saari, Nurhidayu Tanaka, Mami Mino, Sayaka Sawabe, Tomoo Md Yasin, Ina‐Salwany Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia
description	Vibrio infections are common among marine fish and lead to serious problems in the aquaculture sector. This study reports a recent occurrence of Vibrio species (spp.) isolated from cultured groupers in Peninsular Malaysia using the gyrB and pyrH genes. A total of 147 Vibrio strains were successfully isolated from 77 (64%) groupers using culture method and subjected to gyrB and pyrH sequencing for species identifica‐ tion and confirmation. Results showed that 89% of Vibrio strains were identified and clustered to six groups of Vibrio spp., while 11% were not clustered to any Vibrio spp. using the gyrB sequences. Meanwhile, by analysis of the pyrH sequences all the 147 Vibrio strains (100%) were successfully identified and clustered into 11 groups of Vibrio spp., including the gyrB non‐identified strains. The pyrH gene provides a better resolution for identification of Vibrio spp. compared with the gyrB gene. Thus, the pyrH gene was more suitable for a rapid determination of Vibrio spp. distribution in Peninsular Malaysia. Using the pyrH gene, our study found higher prevalence of Vibrio vulnificus (33%), V. alginolyticus (24%) and V. parahaemolyticus (22%), followed by V. rotiferianus (5%), V. harveyi (3%), V. tubiashii (2%), V. campbellii (2%), V. ponticus (1%), V. diabolicus (1%), V. owensii (1%) and others Vibrio sp. (7%). Thus, the results of this study revealed that the occurrence of pathogenic vibrios among grouper fish is still high in Malaysian aquaculture. In addition, the pyrH gene was proved as a suitable marker for rapid identification of Vibrio species compared with the gyrB gene.
format	Article
author	Zulkipli, Nor Amalina Zulperi, Dzarifah Azmai Amal, Mohammad Noor Yusof, Mohd Termizi Saad, Mohd Zamri Al-saari, Nurhidayu Tanaka, Mami Mino, Sayaka Sawabe, Tomoo Md Yasin, Ina‐Salwany
author_facet	Zulkipli, Nor Amalina Zulperi, Dzarifah Azmai Amal, Mohammad Noor Yusof, Mohd Termizi Saad, Mohd Zamri Al-saari, Nurhidayu Tanaka, Mami Mino, Sayaka Sawabe, Tomoo Md Yasin, Ina‐Salwany
author_sort	Zulkipli, Nor Amalina
title	Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia
title_short	Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia
title_full	Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia
title_fullStr	Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia
title_full_unstemmed	Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia
title_sort	recent update on the prevalence of vibrio species among cultured grouper in peninsular malaysia
publisher	Wiley Online Library
publishDate	2019
url	http://irep.iium.edu.my/75657/ http://irep.iium.edu.my/75657/ http://irep.iium.edu.my/75657/ http://irep.iium.edu.my/75657/19/75657%20Recent%20update%20on%20the%20prevalence%20of%20Vibrio.pdf http://irep.iium.edu.my/75657/7/75657_Recent%20update%20on%20the%20prevalence%20of%20Vibrio%20species%20among%20cultured%20grouper%20in%20Peninsular%20Malaysia_Scopus.pdf
first_indexed	2023-09-18T21:47:02Z
last_indexed	2023-09-18T21:47:02Z
_version_	1777413518294777856

Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia

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