Understanding the NaCl-dependent behavior of hydrogen production of a marine bacterium, Vibrio tritonius

Biohydrogen is one of the most suitable clean energy sources for sustaining a fossil fuel independent society. The use of both land and ocean bioresources as feedstocks show great potential in maximizing biohydrogen production, but sodium ion is one of the main obstacles in efficient bacterial biohy...

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Main Authors:	Al-saari, Nurhidayu, Amada, Eri, Matsumura, Yuta, Tanaka, Mami, Mino, Sayaka, Sawabe, Tomoo
Format:	Article
Language:	English English
Published:	PeerJ 2019
Subjects:	QR Microbiology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/75660/ http://irep.iium.edu.my/75660/ http://irep.iium.edu.my/75660/ http://irep.iium.edu.my/75660/7/75660%20Understanding%20the%20NaCl-dependent.pdf http://irep.iium.edu.my/75660/8/75660%20Understanding%20the%20NaCl-dependent%20SCOPUS.pdf

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spelling	iium-756602019-11-25T02:36:26Z http://irep.iium.edu.my/75660/ Understanding the NaCl-dependent behavior of hydrogen production of a marine bacterium, Vibrio tritonius Al-saari, Nurhidayu Amada, Eri Matsumura, Yuta Tanaka, Mami Mino, Sayaka Sawabe, Tomoo QR Microbiology TP248.13 Biotechnology Biohydrogen is one of the most suitable clean energy sources for sustaining a fossil fuel independent society. The use of both land and ocean bioresources as feedstocks show great potential in maximizing biohydrogen production, but sodium ion is one of the main obstacles in efficient bacterial biohydrogen production. Vibrio tritonius strain AM2 can perform efficient hydrogen production with a molar yield of 1.7 mol H2/mol mannitol, which corresponds to 85% theoretical molar yield of H2 production, under saline conditions. With a view to maximizing the hydrogen production using marine biomass, it is important to accumulate knowledge on the effects of salts on the hydrogen production kinetics. Here, we show the kinetics in batch hydrogen production of V. tritonius strain AM2 to investigate the response to various NaCl concentrations. The modified Han–Levenspiel model reveals that salt inhibition in hydrogen production using V. tritonius starts precisely at the point where 10.2 g/L of NaCl is added, and is critically inhibited at 46 g/L. NaCl concentration greatly affects the substrate consumption which in turn affects both growth and hydrogen production. The NaCl-dependent behavior of fermentative hydrogen production of V. tritonius compared to that of Escherichia coli JCM 1649 reveals the marine-adapted fermentative hydrogen production system in V. tritonius. V. tritonius AM2 is capable of producing hydrogen from seaweed carbohydrate under a wide range of NaCl concentrations (5 to 46 g/L). The optimal salt concentration producing the highest levels of hydrogen, optimal substrate consumption and highest molar hydrogen yield is at 10 g/L NaCl (1.0% (w/v)). PeerJ 2019-04-17 Article PeerReviewed application/pdf en http://irep.iium.edu.my/75660/7/75660%20Understanding%20the%20NaCl-dependent.pdf application/pdf en http://irep.iium.edu.my/75660/8/75660%20Understanding%20the%20NaCl-dependent%20SCOPUS.pdf Al-saari, Nurhidayu and Amada, Eri and Matsumura, Yuta and Tanaka, Mami and Mino, Sayaka and Sawabe, Tomoo (2019) Understanding the NaCl-dependent behavior of hydrogen production of a marine bacterium, Vibrio tritonius. PeerJ, 2019. pp. 1-21. ISSN 2167-8359 https://peerj.com/articles/6769.pdf 10.7717/peerj.6769
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology TP248.13 Biotechnology
spellingShingle	QR Microbiology TP248.13 Biotechnology Al-saari, Nurhidayu Amada, Eri Matsumura, Yuta Tanaka, Mami Mino, Sayaka Sawabe, Tomoo Understanding the NaCl-dependent behavior of hydrogen production of a marine bacterium, Vibrio tritonius
description	Biohydrogen is one of the most suitable clean energy sources for sustaining a fossil fuel independent society. The use of both land and ocean bioresources as feedstocks show great potential in maximizing biohydrogen production, but sodium ion is one of the main obstacles in efficient bacterial biohydrogen production. Vibrio tritonius strain AM2 can perform efficient hydrogen production with a molar yield of 1.7 mol H2/mol mannitol, which corresponds to 85% theoretical molar yield of H2 production, under saline conditions. With a view to maximizing the hydrogen production using marine biomass, it is important to accumulate knowledge on the effects of salts on the hydrogen production kinetics. Here, we show the kinetics in batch hydrogen production of V. tritonius strain AM2 to investigate the response to various NaCl concentrations. The modified Han–Levenspiel model reveals that salt inhibition in hydrogen production using V. tritonius starts precisely at the point where 10.2 g/L of NaCl is added, and is critically inhibited at 46 g/L. NaCl concentration greatly affects the substrate consumption which in turn affects both growth and hydrogen production. The NaCl-dependent behavior of fermentative hydrogen production of V. tritonius compared to that of Escherichia coli JCM 1649 reveals the marine-adapted fermentative hydrogen production system in V. tritonius. V. tritonius AM2 is capable of producing hydrogen from seaweed carbohydrate under a wide range of NaCl concentrations (5 to 46 g/L). The optimal salt concentration producing the highest levels of hydrogen, optimal substrate consumption and highest molar hydrogen yield is at 10 g/L NaCl (1.0% (w/v)).
format	Article
author	Al-saari, Nurhidayu Amada, Eri Matsumura, Yuta Tanaka, Mami Mino, Sayaka Sawabe, Tomoo
author_facet	Al-saari, Nurhidayu Amada, Eri Matsumura, Yuta Tanaka, Mami Mino, Sayaka Sawabe, Tomoo
author_sort	Al-saari, Nurhidayu
title	Understanding the NaCl-dependent behavior of hydrogen production of a marine bacterium, Vibrio tritonius
title_short	Understanding the NaCl-dependent behavior of hydrogen production of a marine bacterium, Vibrio tritonius
title_full	Understanding the NaCl-dependent behavior of hydrogen production of a marine bacterium, Vibrio tritonius
title_fullStr	Understanding the NaCl-dependent behavior of hydrogen production of a marine bacterium, Vibrio tritonius
title_full_unstemmed	Understanding the NaCl-dependent behavior of hydrogen production of a marine bacterium, Vibrio tritonius
title_sort	understanding the nacl-dependent behavior of hydrogen production of a marine bacterium, vibrio tritonius
publisher	PeerJ
publishDate	2019
url	http://irep.iium.edu.my/75660/ http://irep.iium.edu.my/75660/ http://irep.iium.edu.my/75660/ http://irep.iium.edu.my/75660/7/75660%20Understanding%20the%20NaCl-dependent.pdf http://irep.iium.edu.my/75660/8/75660%20Understanding%20the%20NaCl-dependent%20SCOPUS.pdf
first_indexed	2023-09-18T21:47:02Z
last_indexed	2023-09-18T21:47:02Z
_version_	1777413518721548288

Understanding the NaCl-dependent behavior of hydrogen production of a marine bacterium, Vibrio tritonius

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