Identification of hyaluric acid synthesis 2 (has2) and gremlin 1 (grem1) gene expressions in human cumulus cells as a biomarker for oocyte quality.

Nowadays, the criteria for oocyte selection is based on morphological criteria however, it needs further improvement to select the best embryo quality. The gene expression in the cumulus cell plays important role in signaling for follicular development as well as for oocyte quality. The aim of this...

Full description

Bibliographic Details
Main Authors:	Abdul Wahab, Azantee Yazmie, Md Isa, Muhammad Lokman, Mohd Yassim, Haiyuni, Salleh, Yukmil Amru, Mat Yusof, Afzan, Abdul Razak, Redzuan Nul Hakim, Abdul Razak, Nik Fakhuruddin, Muhammad, Hussin, Mazlan, Muhammad Alif
Format:	Article
Language:	English English
Published:	Malaysian Society for Biochemistry and Molecular Biology (MSBMB) 2019
Subjects:	R Medicine (General) RG Gynecology and obstetrics RT Nursing
Online Access:	http://irep.iium.edu.my/76226/ http://irep.iium.edu.my/76226/ http://irep.iium.edu.my/76226/1/76226_Identification%20of%20Hyaluric%20Acid%20synthesis%202.pdf http://irep.iium.edu.my/76226/7/76226_Identification%20of%20hyaluric%20acid%20synthesis%202%20%28HAS2%29%20and%20gremlin%201%20%28GREM1%29%20gene%20expressions%20in%20human%20cumulus%20cells%20as%20a%20biomarker%20for%20oocyte%20quality_Scopus.pdf

id	iium-76226
recordtype	eprints
spelling	iium-762262020-02-27T07:23:26Z http://irep.iium.edu.my/76226/ Identification of hyaluric acid synthesis 2 (has2) and gremlin 1 (grem1) gene expressions in human cumulus cells as a biomarker for oocyte quality. Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Mohd Yassim, Haiyuni Salleh, Yukmil Amru Mat Yusof, Afzan Abdul Razak, Redzuan Nul Hakim Abdul Razak, Nik Fakhuruddin Muhammad, Hussin Mazlan, Muhammad Alif R Medicine (General) RG Gynecology and obstetrics RT Nursing Nowadays, the criteria for oocyte selection is based on morphological criteria however, it needs further improvement to select the best embryo quality. The gene expression in the cumulus cell plays important role in signaling for follicular development as well as for oocyte quality. The aim of this study is to investigate the present of HAS2 and GREM1 gene expression in the cumulus cells that can become a useful marker for oocyte quality. A preliminary study was performed on cumulus cells derived from four different patients that undergo assisted reproductive technique treatment. Cumulus cells were isolated from 4 patients and the expression of HAS2 and GREM1 was analyzed by using reverse transcriptase polymerase chain reaction (rt- PCR). The results shown that HAS2 and GREM1 were expressed in grade 3 oocytes, whereas, the genes were absent in grade 4 oocytes. This showed that the expression influenced the oocyte quality. Hence, the measurement of HAS2 and GREM1 expressions in cumulus cells would reliably useful tool for selecting competence oocytes with greater chances to be fertilized in assisted reproductive technique. Malaysian Society for Biochemistry and Molecular Biology (MSBMB) 2019-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/76226/1/76226_Identification%20of%20Hyaluric%20Acid%20synthesis%202.pdf application/pdf en http://irep.iium.edu.my/76226/7/76226_Identification%20of%20hyaluric%20acid%20synthesis%202%20%28HAS2%29%20and%20gremlin%201%20%28GREM1%29%20gene%20expressions%20in%20human%20cumulus%20cells%20as%20a%20biomarker%20for%20oocyte%20quality_Scopus.pdf Abdul Wahab, Azantee Yazmie and Md Isa, Muhammad Lokman and Mohd Yassim, Haiyuni and Salleh, Yukmil Amru and Mat Yusof, Afzan and Abdul Razak, Redzuan Nul Hakim and Abdul Razak, Nik Fakhuruddin and Muhammad, Hussin and Mazlan, Muhammad Alif (2019) Identification of hyaluric acid synthesis 2 (has2) and gremlin 1 (grem1) gene expressions in human cumulus cells as a biomarker for oocyte quality. Malaysian Journal of Biochemstry and Molecular Biology, 22 (2). pp. 41-45. E-ISSN 2600-9005 https://drive.google.com/file/d/1Pgy1W5AC6pEMal9Aw9AaHCTXvNN0zF_9/view
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	R Medicine (General) RG Gynecology and obstetrics RT Nursing
spellingShingle	R Medicine (General) RG Gynecology and obstetrics RT Nursing Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Mohd Yassim, Haiyuni Salleh, Yukmil Amru Mat Yusof, Afzan Abdul Razak, Redzuan Nul Hakim Abdul Razak, Nik Fakhuruddin Muhammad, Hussin Mazlan, Muhammad Alif Identification of hyaluric acid synthesis 2 (has2) and gremlin 1 (grem1) gene expressions in human cumulus cells as a biomarker for oocyte quality.
description	Nowadays, the criteria for oocyte selection is based on morphological criteria however, it needs further improvement to select the best embryo quality. The gene expression in the cumulus cell plays important role in signaling for follicular development as well as for oocyte quality. The aim of this study is to investigate the present of HAS2 and GREM1 gene expression in the cumulus cells that can become a useful marker for oocyte quality. A preliminary study was performed on cumulus cells derived from four different patients that undergo assisted reproductive technique treatment. Cumulus cells were isolated from 4 patients and the expression of HAS2 and GREM1 was analyzed by using reverse transcriptase polymerase chain reaction (rt- PCR). The results shown that HAS2 and GREM1 were expressed in grade 3 oocytes, whereas, the genes were absent in grade 4 oocytes. This showed that the expression influenced the oocyte quality. Hence, the measurement of HAS2 and GREM1 expressions in cumulus cells would reliably useful tool for selecting competence oocytes with greater chances to be fertilized in assisted reproductive technique.
format	Article
author	Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Mohd Yassim, Haiyuni Salleh, Yukmil Amru Mat Yusof, Afzan Abdul Razak, Redzuan Nul Hakim Abdul Razak, Nik Fakhuruddin Muhammad, Hussin Mazlan, Muhammad Alif
author_facet	Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Mohd Yassim, Haiyuni Salleh, Yukmil Amru Mat Yusof, Afzan Abdul Razak, Redzuan Nul Hakim Abdul Razak, Nik Fakhuruddin Muhammad, Hussin Mazlan, Muhammad Alif
author_sort	Abdul Wahab, Azantee Yazmie
title	Identification of hyaluric acid synthesis 2 (has2) and gremlin 1 (grem1) gene expressions in human cumulus cells as a biomarker for oocyte quality.
title_short	Identification of hyaluric acid synthesis 2 (has2) and gremlin 1 (grem1) gene expressions in human cumulus cells as a biomarker for oocyte quality.
title_full	Identification of hyaluric acid synthesis 2 (has2) and gremlin 1 (grem1) gene expressions in human cumulus cells as a biomarker for oocyte quality.
title_fullStr	Identification of hyaluric acid synthesis 2 (has2) and gremlin 1 (grem1) gene expressions in human cumulus cells as a biomarker for oocyte quality.
title_full_unstemmed	Identification of hyaluric acid synthesis 2 (has2) and gremlin 1 (grem1) gene expressions in human cumulus cells as a biomarker for oocyte quality.
title_sort	identification of hyaluric acid synthesis 2 (has2) and gremlin 1 (grem1) gene expressions in human cumulus cells as a biomarker for oocyte quality.
publisher	Malaysian Society for Biochemistry and Molecular Biology (MSBMB)
publishDate	2019
url	http://irep.iium.edu.my/76226/ http://irep.iium.edu.my/76226/ http://irep.iium.edu.my/76226/1/76226_Identification%20of%20Hyaluric%20Acid%20synthesis%202.pdf http://irep.iium.edu.my/76226/7/76226_Identification%20of%20hyaluric%20acid%20synthesis%202%20%28HAS2%29%20and%20gremlin%201%20%28GREM1%29%20gene%20expressions%20in%20human%20cumulus%20cells%20as%20a%20biomarker%20for%20oocyte%20quality_Scopus.pdf
first_indexed	2023-09-18T21:47:44Z
last_indexed	2023-09-18T21:47:44Z
_version_	1777413563150761984

Identification of hyaluric acid synthesis 2 (has2) and gremlin 1 (grem1) gene expressions in human cumulus cells as a biomarker for oocyte quality.

Similar Items