Chlorella vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes muscle regeneration

Sarcopenia is characterized by the loss of muscle mass, strength, and function with ageing. With increasing life expectancy, greater attention has been given to counteracting the effects of sarcopenia on the growing elderly population. Chlorella vulgaris, a microscopic, unicellular, green alga with...

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Main Authors:	Zainul Azlan, Nurhazirah, Mohd Yusof, Yasmin Anum, Alias, Ekram, Makpol, Suzana
Format:	Article
Language:	English English English
Published:	Hindawi 2019
Subjects:	RZ Other systems of medicine
Online Access:	http://irep.iium.edu.my/77215/ http://irep.iium.edu.my/77215/ http://irep.iium.edu.my/77215/ http://irep.iium.edu.my/77215/1/77215_Chlorella%20vulgaris%20Improves%20the%20Regenerative.pdf http://irep.iium.edu.my/77215/2/77215_Chlorella%20vulgaris%20Improves%20the%20Regenerative_SCOPUS.pdf http://irep.iium.edu.my/77215/3/77215_Chlorella%20vulgaris%20Improves%20the%20Regenerative_WOS.pdf

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spelling	iium-772152020-02-05T01:06:57Z http://irep.iium.edu.my/77215/ Chlorella vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes muscle regeneration Zainul Azlan, Nurhazirah Mohd Yusof, Yasmin Anum Alias, Ekram Makpol, Suzana RZ Other systems of medicine Sarcopenia is characterized by the loss of muscle mass, strength, and function with ageing. With increasing life expectancy, greater attention has been given to counteracting the effects of sarcopenia on the growing elderly population. Chlorella vulgaris, a microscopic, unicellular, green alga with the potential for various pharmaceutical uses, has been widely studied in this context. This study is aimed at determining the effects of C. vulgaris on promoting muscle regeneration by evaluating myoblast regenerative capacity in vitro. Human skeletal myoblast cells were cultured and underwent serial passaging into young and senescent phases and were then treated with C. vulgaris, followed by the induction of differentiation. The ability of C. vulgaris to promote myoblast differentiation was analysed through cellular morphology, real-time monitoring, cell proliferation, senescence-associated β-galactosidase (SA-β-gal) expression, myogenic differentiation, myogenin expression, and cell cycle profiling. The results obtained showed that senescent myoblasts exhibited an enlarged and flattened morphology, with increased SA-β-gal expression, reduced myogenic differentiation, decreased expression of myogenin, and an increased percentage of cells in the G0/G1 phase. Treatment with C. vulgaris resulted in decreased SA-β-gal expression and promotion of myogenic differentiation, as observed via an increased fusion index, maturation index, myotube size, and surface area and an increased percentage of cells that stained positive for myogenin. In conclusion, C. vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes myoblast differentiation, indicating its potential to promote muscle regeneration. Hindawi 2019-06-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/77215/1/77215_Chlorella%20vulgaris%20Improves%20the%20Regenerative.pdf application/pdf en http://irep.iium.edu.my/77215/2/77215_Chlorella%20vulgaris%20Improves%20the%20Regenerative_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/77215/3/77215_Chlorella%20vulgaris%20Improves%20the%20Regenerative_WOS.pdf Zainul Azlan, Nurhazirah and Mohd Yusof, Yasmin Anum and Alias, Ekram and Makpol, Suzana (2019) Chlorella vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes muscle regeneration. Oxidative Medicine and Cellular Longevity, 2019. pp. 1-16. ISSN 1942-0900 E-ISSN 1942-0994 http://downloads.hindawi.com/journals/omcl/2019/3520789.pdf https://doi.org/10.1155/2019/3520789
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RZ Other systems of medicine
spellingShingle	RZ Other systems of medicine Zainul Azlan, Nurhazirah Mohd Yusof, Yasmin Anum Alias, Ekram Makpol, Suzana Chlorella vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes muscle regeneration
description	Sarcopenia is characterized by the loss of muscle mass, strength, and function with ageing. With increasing life expectancy, greater attention has been given to counteracting the effects of sarcopenia on the growing elderly population. Chlorella vulgaris, a microscopic, unicellular, green alga with the potential for various pharmaceutical uses, has been widely studied in this context. This study is aimed at determining the effects of C. vulgaris on promoting muscle regeneration by evaluating myoblast regenerative capacity in vitro. Human skeletal myoblast cells were cultured and underwent serial passaging into young and senescent phases and were then treated with C. vulgaris, followed by the induction of differentiation. The ability of C. vulgaris to promote myoblast differentiation was analysed through cellular morphology, real-time monitoring, cell proliferation, senescence-associated β-galactosidase (SA-β-gal) expression, myogenic differentiation, myogenin expression, and cell cycle profiling. The results obtained showed that senescent myoblasts exhibited an enlarged and flattened morphology, with increased SA-β-gal expression, reduced myogenic differentiation, decreased expression of myogenin, and an increased percentage of cells in the G0/G1 phase. Treatment with C. vulgaris resulted in decreased SA-β-gal expression and promotion of myogenic differentiation, as observed via an increased fusion index, maturation index, myotube size, and surface area and an increased percentage of cells that stained positive for myogenin. In conclusion, C. vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes myoblast differentiation, indicating its potential to promote muscle regeneration.
format	Article
author	Zainul Azlan, Nurhazirah Mohd Yusof, Yasmin Anum Alias, Ekram Makpol, Suzana
author_facet	Zainul Azlan, Nurhazirah Mohd Yusof, Yasmin Anum Alias, Ekram Makpol, Suzana
author_sort	Zainul Azlan, Nurhazirah
title	Chlorella vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes muscle regeneration
title_short	Chlorella vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes muscle regeneration
title_full	Chlorella vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes muscle regeneration
title_fullStr	Chlorella vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes muscle regeneration
title_full_unstemmed	Chlorella vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes muscle regeneration
title_sort	chlorella vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes muscle regeneration
publisher	Hindawi
publishDate	2019
url	http://irep.iium.edu.my/77215/ http://irep.iium.edu.my/77215/ http://irep.iium.edu.my/77215/ http://irep.iium.edu.my/77215/1/77215_Chlorella%20vulgaris%20Improves%20the%20Regenerative.pdf http://irep.iium.edu.my/77215/2/77215_Chlorella%20vulgaris%20Improves%20the%20Regenerative_SCOPUS.pdf http://irep.iium.edu.my/77215/3/77215_Chlorella%20vulgaris%20Improves%20the%20Regenerative_WOS.pdf
first_indexed	2023-09-18T21:48:56Z
last_indexed	2023-09-18T21:48:56Z
_version_	1777413638149111808

Chlorella vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes muscle regeneration

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