Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study

Introduction: Monosodium methylarsonate (MSMA) is an organic arsenical pesticide widely used in agriculture. Humans are exposed to arsenic through drinking water and anthropogenic activities. Exposure to inorganic arsenic has been linked with multiple health problems. However, studies focusing on ch...

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Main Authors:	Wan Salleh, Wan Muhamad Salahudin, A.Talib, Norlelawati, Abdullah, Nor Zamzila, Aung, Sanda, Buyong, Zunariah
Format:	Conference or Workshop Item
Language:	English English English
Published:	2019
Subjects:	RA1190 Toxicology
Online Access:	http://irep.iium.edu.my/77426/ http://irep.iium.edu.my/77426/1/MRS%202019%20abstract.pdf http://irep.iium.edu.my/77426/2/Frontpage%20MRS%20and%20Abstract.pdf http://irep.iium.edu.my/77426/3/MRS%20drsolah%202019%20v2.pdf

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recordtype	eprints
spelling	iium-774262020-02-04T00:24:43Z http://irep.iium.edu.my/77426/ Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study Wan Salleh, Wan Muhamad Salahudin A.Talib, Norlelawati Abdullah, Nor Zamzila Aung, Sanda Buyong, Zunariah RA1190 Toxicology Introduction: Monosodium methylarsonate (MSMA) is an organic arsenical pesticide widely used in agriculture. Humans are exposed to arsenic through drinking water and anthropogenic activities. Exposure to inorganic arsenic has been linked with multiple health problems. However, studies focusing on chronic organic arsenic exposure and its adverse effects on kidney were limited. The purpose of current study was to determine the effects of chronic organic arsenic exposure in rats kidney by light and electron microscopy. Materials and Method: Thirty-six male SpragueDawley rats were divided into six groups (n=6); three control and three treatment group respectively. All the control group was given distilled water via oral gavage.The treatment group was given oral gavage of MSMA at 42.10 mg/kg body weight (BW) which is equivalent 1/30 LD50 of MSMA . The control and treatment groups were sacrificed at two month, four month and six months interval. Both kidneys harvested for light microscopy and electron microscopy study. Results: Showed progressive changes. The changes initially focal and became diffused involving glomerular; such as glomerular hypercellularity, glomerular shrinkage and dilated Bowman's space. Meanwhile, in proximal tubules, showed diminished brush borders, detachment of nucleus and basement membrane thickening. Electron microscopy showed flattened cell bodies of podocytes, effacement and fusion of podocytes foot processes, thickening of glomerular basement membrane, and discontinuity of brush border. The control and two-months treated group appeared to be normal.Conclusion: Chronic organic arsenic (MSMA) exposure induced chronic kidney injury. 2019-10-09 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/77426/1/MRS%202019%20abstract.pdf application/pdf en http://irep.iium.edu.my/77426/2/Frontpage%20MRS%20and%20Abstract.pdf application/pdf en http://irep.iium.edu.my/77426/3/MRS%20drsolah%202019%20v2.pdf Wan Salleh, Wan Muhamad Salahudin and A.Talib, Norlelawati and Abdullah, Nor Zamzila and Aung, Sanda and Buyong, Zunariah (2019) Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study. In: 5th Medical Research Symposium 2019, 9th October 2019, IIUM Medical Centre, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RA1190 Toxicology
spellingShingle	RA1190 Toxicology Wan Salleh, Wan Muhamad Salahudin A.Talib, Norlelawati Abdullah, Nor Zamzila Aung, Sanda Buyong, Zunariah Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study
description	Introduction: Monosodium methylarsonate (MSMA) is an organic arsenical pesticide widely used in agriculture. Humans are exposed to arsenic through drinking water and anthropogenic activities. Exposure to inorganic arsenic has been linked with multiple health problems. However, studies focusing on chronic organic arsenic exposure and its adverse effects on kidney were limited. The purpose of current study was to determine the effects of chronic organic arsenic exposure in rats kidney by light and electron microscopy. Materials and Method: Thirty-six male SpragueDawley rats were divided into six groups (n=6); three control and three treatment group respectively. All the control group was given distilled water via oral gavage.The treatment group was given oral gavage of MSMA at 42.10 mg/kg body weight (BW) which is equivalent 1/30 LD50 of MSMA . The control and treatment groups were sacrificed at two month, four month and six months interval. Both kidneys harvested for light microscopy and electron microscopy study. Results: Showed progressive changes. The changes initially focal and became diffused involving glomerular; such as glomerular hypercellularity, glomerular shrinkage and dilated Bowman's space. Meanwhile, in proximal tubules, showed diminished brush borders, detachment of nucleus and basement membrane thickening. Electron microscopy showed flattened cell bodies of podocytes, effacement and fusion of podocytes foot processes, thickening of glomerular basement membrane, and discontinuity of brush border. The control and two-months treated group appeared to be normal.Conclusion: Chronic organic arsenic (MSMA) exposure induced chronic kidney injury.
format	Conference or Workshop Item
author	Wan Salleh, Wan Muhamad Salahudin A.Talib, Norlelawati Abdullah, Nor Zamzila Aung, Sanda Buyong, Zunariah
author_facet	Wan Salleh, Wan Muhamad Salahudin A.Talib, Norlelawati Abdullah, Nor Zamzila Aung, Sanda Buyong, Zunariah
author_sort	Wan Salleh, Wan Muhamad Salahudin
title	Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study
title_short	Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study
title_full	Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study
title_fullStr	Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study
title_full_unstemmed	Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study
title_sort	effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study
publishDate	2019
url	http://irep.iium.edu.my/77426/ http://irep.iium.edu.my/77426/1/MRS%202019%20abstract.pdf http://irep.iium.edu.my/77426/2/Frontpage%20MRS%20and%20Abstract.pdf http://irep.iium.edu.my/77426/3/MRS%20drsolah%202019%20v2.pdf
first_indexed	2023-09-18T21:49:12Z
last_indexed	2023-09-18T21:49:12Z
_version_	1777413655454810112

Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study

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