Chronic exposure of low dose organic arsenic induced microscopic changes in rat’s kidney

Introduction: Monosodium methylarsonate (MSMA) is an organic arsenic widely used in agriculture. Humans are exposed to arsenic through drinking water and anthropogenic activities. Chronic exposure to inorganic arsenic has been linked with multiple health problems. However, studies focusing on organi...

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Main Authors:	Wan Salleh, Wan Muhamad Salahudin, A.Talib, Norlelawati, Abdullah, Nor Zamzila, Aung, Sanda, Buyong, Zunariah
Format:	Conference or Workshop Item
Language:	English English English
Published:	2019
Subjects:	RA1190 Toxicology
Online Access:	http://irep.iium.edu.my/77438/ http://irep.iium.edu.my/77438/1/Cover%20Page%20SCMSM.pdf http://irep.iium.edu.my/77438/2/List%20Poster%20Presenter.pdf http://irep.iium.edu.my/77438/3/microscopy%20conference%20poster%20v2.pdf

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recordtype	eprints
spelling	iium-774382020-02-04T00:22:20Z http://irep.iium.edu.my/77438/ Chronic exposure of low dose organic arsenic induced microscopic changes in rat’s kidney Wan Salleh, Wan Muhamad Salahudin A.Talib, Norlelawati Abdullah, Nor Zamzila Aung, Sanda Buyong, Zunariah RA1190 Toxicology Introduction: Monosodium methylarsonate (MSMA) is an organic arsenic widely used in agriculture. Humans are exposed to arsenic through drinking water and anthropogenic activities. Chronic exposure to inorganic arsenic has been linked with multiple health problems. However, studies focusing on organic arsenic effects on kidneys were limited. Objective: To determine the effects of organic arsenic exposure on rat’s kidney. Materials and Method: Twelve male Sprague-Dawley rats were divided into control (n=6) and treated (n=6) groups. Control group was given daily oral gavage of distilled water and treated group was given daily oral gavage of MSMA at 63.0 mg/kg body weight, which was equivalent of 1/30 LD50 of MSMA. After four months, rats urines were collected using metabolic cage and both groups were sacrificed. The urines were analyzed using enzyme-linked immunosorbent assay (ELISA) for neutrophil gelatinase-associated lipocalin (NGAL) biomarkers for kidney injury. The kidneys were harvested for light microscopy study. Results: Urine NGAL significantly higher (p-value <0.05) in treated groups. Light microscopy showed focal glomerular and tubular changes in treated groups. Conclusion: Chronic low dose organic arsenic exposure induced kidney injury. 2019-12-18 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/77438/1/Cover%20Page%20SCMSM.pdf application/pdf en http://irep.iium.edu.my/77438/2/List%20Poster%20Presenter.pdf application/pdf en http://irep.iium.edu.my/77438/3/microscopy%20conference%20poster%20v2.pdf Wan Salleh, Wan Muhamad Salahudin and A.Talib, Norlelawati and Abdullah, Nor Zamzila and Aung, Sanda and Buyong, Zunariah (2019) Chronic exposure of low dose organic arsenic induced microscopic changes in rat’s kidney. In: 28th Scientific Conference of Microscopy Society Malaysia, 18th - 20th December 2019, Swiss Garden Beach Resort, Kuantan Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RA1190 Toxicology
spellingShingle	RA1190 Toxicology Wan Salleh, Wan Muhamad Salahudin A.Talib, Norlelawati Abdullah, Nor Zamzila Aung, Sanda Buyong, Zunariah Chronic exposure of low dose organic arsenic induced microscopic changes in rat’s kidney
description	Introduction: Monosodium methylarsonate (MSMA) is an organic arsenic widely used in agriculture. Humans are exposed to arsenic through drinking water and anthropogenic activities. Chronic exposure to inorganic arsenic has been linked with multiple health problems. However, studies focusing on organic arsenic effects on kidneys were limited. Objective: To determine the effects of organic arsenic exposure on rat’s kidney. Materials and Method: Twelve male Sprague-Dawley rats were divided into control (n=6) and treated (n=6) groups. Control group was given daily oral gavage of distilled water and treated group was given daily oral gavage of MSMA at 63.0 mg/kg body weight, which was equivalent of 1/30 LD50 of MSMA. After four months, rats urines were collected using metabolic cage and both groups were sacrificed. The urines were analyzed using enzyme-linked immunosorbent assay (ELISA) for neutrophil gelatinase-associated lipocalin (NGAL) biomarkers for kidney injury. The kidneys were harvested for light microscopy study. Results: Urine NGAL significantly higher (p-value <0.05) in treated groups. Light microscopy showed focal glomerular and tubular changes in treated groups. Conclusion: Chronic low dose organic arsenic exposure induced kidney injury.
format	Conference or Workshop Item
author	Wan Salleh, Wan Muhamad Salahudin A.Talib, Norlelawati Abdullah, Nor Zamzila Aung, Sanda Buyong, Zunariah
author_facet	Wan Salleh, Wan Muhamad Salahudin A.Talib, Norlelawati Abdullah, Nor Zamzila Aung, Sanda Buyong, Zunariah
author_sort	Wan Salleh, Wan Muhamad Salahudin
title	Chronic exposure of low dose organic arsenic induced microscopic changes in rat’s kidney
title_short	Chronic exposure of low dose organic arsenic induced microscopic changes in rat’s kidney
title_full	Chronic exposure of low dose organic arsenic induced microscopic changes in rat’s kidney
title_fullStr	Chronic exposure of low dose organic arsenic induced microscopic changes in rat’s kidney
title_full_unstemmed	Chronic exposure of low dose organic arsenic induced microscopic changes in rat’s kidney
title_sort	chronic exposure of low dose organic arsenic induced microscopic changes in rat’s kidney
publishDate	2019
url	http://irep.iium.edu.my/77438/ http://irep.iium.edu.my/77438/1/Cover%20Page%20SCMSM.pdf http://irep.iium.edu.my/77438/2/List%20Poster%20Presenter.pdf http://irep.iium.edu.my/77438/3/microscopy%20conference%20poster%20v2.pdf
first_indexed	2023-09-18T21:49:13Z
last_indexed	2023-09-18T21:49:13Z
_version_	1777413656011603968

Chronic exposure of low dose organic arsenic induced microscopic changes in rat’s kidney

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