$Extraction, analysis, anti-tyrosinase activity and formulation of asiaticoside rich fraction from Centella asiatica$

Extraction, analysis, anti-tyrosinase activity and formulation of asiaticoside rich fraction from Centella asiatica

Introduction: The bioactive component of Centella asiatica (L) Urban, asiaticoside is known to exhibit many therapeutic effects. Objectives: This study aimed to develop asiaticoside fractionation method using activated charcoal, evaluate the bioactivity on melanogenesis of the fraction, as well as t...

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Main Authors:	Sabar, Aina Mardhiah, Bakhtiar, M. Taher, Amri, Mohamad Shahreen, Darnis, Deny Susanti
Format:	Conference or Workshop Item
Language:	English English
Published:	2019
Subjects:	RS192 Materia Medica-Pharmaceutical Technology
Online Access:	http://irep.iium.edu.my/77822/ http://irep.iium.edu.my/77822/1/ICPRP-2019-ALL-Abstracts.pdf http://irep.iium.edu.my/77822/7/Poster%20Presentation%20anti-tyrosinase.pdf

id	iium-77822
recordtype	eprints
spelling	iium-778222020-01-22T08:20:49Z http://irep.iium.edu.my/77822/ Extraction, analysis, anti-tyrosinase activity and formulation of asiaticoside rich fraction from Centella asiatica Sabar, Aina Mardhiah Bakhtiar, M. Taher Amri, Mohamad Shahreen Darnis, Deny Susanti RS192 Materia Medica-Pharmaceutical Technology Introduction: The bioactive component of Centella asiatica (L) Urban, asiaticoside is known to exhibit many therapeutic effects. Objectives: This study aimed to develop asiaticoside fractionation method using activated charcoal, evaluate the bioactivity on melanogenesis of the fraction, as well as the optimization and characterization of asiaticoside rich fraction from C. asiatica (CAEMD) hydrogel. Methods: TLC, HPLC, and LC-DAD-ESI-ITMS in positive ion mode were adopted to evaluate asiaticoside in CAEMD fraction qualitatively and quantitatively. Results: The concentration of asiaticoside found was 11.27 % of the CAEMD fraction. In LC-DAD-ESI-ITMS, asiaticoside was found the second most abundant compound in CAEMD and the main fragment ions formed were at m/z 976.25 and m/z 453.33derived from [M+NH4]+ and [M+H-Glu-Glu-Rha-2H2O] respectively. The anti-tyrosinase activity of CAEMD fraction had increased by two-fold compared to the crude extract (p < 0.05) while asiaticoside alone did not show any activity. Carbopol 940 gel containing CAEMD was successfully optimized using three-factor, three-level Box-Behnken design, and C940 0.6 %, TEA 0.27 %, and mixing speed at 650 rpm were selected as the optimized condition. Conclusion: CAEMD fraction has a promising effect on inhibiting melanogenesis pathway and the CAEMD formulation has successfully developed in this study. 2019 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/77822/1/ICPRP-2019-ALL-Abstracts.pdf application/pdf en http://irep.iium.edu.my/77822/7/Poster%20Presentation%20anti-tyrosinase.pdf Sabar, Aina Mardhiah and Bakhtiar, M. Taher and Amri, Mohamad Shahreen and Darnis, Deny Susanti (2019) Extraction, analysis, anti-tyrosinase activity and formulation of asiaticoside rich fraction from Centella asiatica. In: International Conference on Pharmaceutical Research and Pharmacy Practice cum 14th IIUM-MPS Pharmacy Scientific Conference (ICPRP 2019), 19th-20th October 2019, Hotel Istana Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS192 Materia Medica-Pharmaceutical Technology
spellingShingle	RS192 Materia Medica-Pharmaceutical Technology Sabar, Aina Mardhiah Bakhtiar, M. Taher Amri, Mohamad Shahreen Darnis, Deny Susanti Extraction, analysis, anti-tyrosinase activity and formulation of asiaticoside rich fraction from Centella asiatica
description	Introduction: The bioactive component of Centella asiatica (L) Urban, asiaticoside is known to exhibit many therapeutic effects. Objectives: This study aimed to develop asiaticoside fractionation method using activated charcoal, evaluate the bioactivity on melanogenesis of the fraction, as well as the optimization and characterization of asiaticoside rich fraction from C. asiatica (CAEMD) hydrogel. Methods: TLC, HPLC, and LC-DAD-ESI-ITMS in positive ion mode were adopted to evaluate asiaticoside in CAEMD fraction qualitatively and quantitatively. Results: The concentration of asiaticoside found was 11.27 % of the CAEMD fraction. In LC-DAD-ESI-ITMS, asiaticoside was found the second most abundant compound in CAEMD and the main fragment ions formed were at m/z 976.25 and m/z 453.33derived from [M+NH4]+ and [M+H-Glu-Glu-Rha-2H2O] respectively. The anti-tyrosinase activity of CAEMD fraction had increased by two-fold compared to the crude extract (p < 0.05) while asiaticoside alone did not show any activity. Carbopol 940 gel containing CAEMD was successfully optimized using three-factor, three-level Box-Behnken design, and C940 0.6 %, TEA 0.27 %, and mixing speed at 650 rpm were selected as the optimized condition. Conclusion: CAEMD fraction has a promising effect on inhibiting melanogenesis pathway and the CAEMD formulation has successfully developed in this study.
format	Conference or Workshop Item
author	Sabar, Aina Mardhiah Bakhtiar, M. Taher Amri, Mohamad Shahreen Darnis, Deny Susanti
author_facet	Sabar, Aina Mardhiah Bakhtiar, M. Taher Amri, Mohamad Shahreen Darnis, Deny Susanti
author_sort	Sabar, Aina Mardhiah
title	Extraction, analysis, anti-tyrosinase activity and formulation of asiaticoside rich fraction from Centella asiatica
title_short	Extraction, analysis, anti-tyrosinase activity and formulation of asiaticoside rich fraction from Centella asiatica
title_full	Extraction, analysis, anti-tyrosinase activity and formulation of asiaticoside rich fraction from Centella asiatica
title_fullStr	Extraction, analysis, anti-tyrosinase activity and formulation of asiaticoside rich fraction from Centella asiatica
title_full_unstemmed	Extraction, analysis, anti-tyrosinase activity and formulation of asiaticoside rich fraction from Centella asiatica
title_sort	extraction, analysis, anti-tyrosinase activity and formulation of asiaticoside rich fraction from centella asiatica
publishDate	2019
url	http://irep.iium.edu.my/77822/ http://irep.iium.edu.my/77822/1/ICPRP-2019-ALL-Abstracts.pdf http://irep.iium.edu.my/77822/7/Poster%20Presentation%20anti-tyrosinase.pdf
first_indexed	2023-09-18T21:49:43Z
last_indexed	2023-09-18T21:49:43Z
_version_	1777413687409115136

Extraction, analysis, anti-tyrosinase activity and formulation of asiaticoside rich fraction from Centella asiatica

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