Role of Vascular Endothelial Growth Factor (VEGF) and doppler sub-endometrial parameters as predictors of successful implantation in Intracytoplasmic Sperm Injection (ICSI) patients
Objective: to investigate the expression of vascular endothelial growth factor (VEGF) in patients having infertility due to low endometrial acceptance, and to correlate it to non-invasive ultrasound variables, endometrial thickness, and sub-endometrial Doppler parameters (PI, RI, Vs/Vd). Methods:...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2020
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/78028/ http://irep.iium.edu.my/78028/ http://irep.iium.edu.my/78028/ http://irep.iium.edu.my/78028/1/78028_Role%20of%20Vascular%20Endothelial%20Growth%20Factor%20%28VEGF%29.pdf |
| Summary: | Objective: to investigate the expression of vascular endothelial growth factor (VEGF) in patients having infertility
due to low endometrial acceptance, and to correlate it to non-invasive ultrasound variables, endometrial thickness,
and sub-endometrial Doppler parameters (PI, RI, Vs/Vd).
Methods: 80 women all under the age of 40 underwent ICSI-ET; all patients were exposed to ovarian stimulation
protocols. The oocytes were retrieved using an ultrasound guide, and were fertilised via injection of sperm inside
the follicle (ICSI). VEGF serum level was analysed at day of embryo transfer by ELIZA test, and sub-endometrial
evaluation was conducted via two-dimension power Doppler ultrasound (2D PD-US), by measuring resistance
index (RI) and pulsatility index (PI) on the day of embryo transfer.
Results: There was a significantly higher VEGF level and endometrial thickness in pregnant (433± 207 and 9.72±
1.35) women, compared to non-pregnant (276± 165 and 8.95 ± 1.21) respectively as p-values were (0.001 and
0.01)). Additionally, there were significantly lower RI and PI in pregnant (0.584 ± 0.124 and 0.829 ± 0.301) women
compared to non-pregnant (0.651±0.132 and 1.006±0.335) women, as p-values were (0.02 and 0.02, respectively).
The level of E2 was on the day of embryo transfer and Vs/Vd in pregnant women (1402± 524 and 3.14 ± 3.75) and
in the non-pregnant group (1296± 611 and 3.82 ± 3.07), as p-values were 0.41 and 0.38, respectively.
Conclusion: The combined analysis of endometrial receptivity was completed, and the serum level of VEGF and
sub-endometrial evaluation with 2D PD-US was defined by measuring resistance index (RI) and pulsatility index
(PI) on the day of embryo transfer. These can serve as useful prognostic methods for the detection of endometrial
receptivity and pregnancy outcomes in infertile women undergoing ICSI protocols, and will be helpful for candidate
counselling about postponing embryo transfer and cryopreservation, which may serve as a better option, to be
recommended for the next cycle, when achieving better endometrial Doppler parameters. |
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