In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers

Mutation breeding techniques in combination with tissue culture and molecular marker methods provide a powerful tool for improvement of vegetatively propagated plants. The aim of this study was to develop a protocol for shoot regeneration and mutation induction of Etlingera elatior. The results of...

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Main Authors:	Yunus, Muhamad Fahmi, Abd Aziz, Maheran, Abdul Kadir, Mihdzar, Daud, Siti Khalijah, Abdul Rashid, Azmi
Format:	Article
Language:	English
Published:	Scientific and Technological Research Council of Turkey 2013
Subjects:	SB Plant culture
Online Access:	http://irep.iium.edu.my/78033/ http://irep.iium.edu.my/78033/ http://irep.iium.edu.my/78033/ http://irep.iium.edu.my/78033/1/In%20vitro%20mutagenesis%20of%20Etlingera%20elatior%20%28Jack%29.pdf

id	iium-78033
recordtype	eprints
spelling	iium-780332020-02-18T01:25:29Z http://irep.iium.edu.my/78033/ In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers Yunus, Muhamad Fahmi Abd Aziz, Maheran Abdul Kadir, Mihdzar Daud, Siti Khalijah Abdul Rashid, Azmi SB Plant culture Mutation breeding techniques in combination with tissue culture and molecular marker methods provide a powerful tool for improvement of vegetatively propagated plants. The aim of this study was to develop a protocol for shoot regeneration and mutation induction of Etlingera elatior. The results of irradiation on in vitro buds of E. elatior showed LD50 to be 10 Gy, with the survival of explants being sharply reduced at this dosage. All 8 selected gamma irradiated regenerants were differentiated from the untreated control based on the banding patterns obtained using 9 primers, which generated 59 reproducible bands, whereby 35 (55.31%) were found to be polymorphic. Jaccard’s coefficient of similarity values ranging from 0.537 to 0.860 were indicative of the level of genetic variation among the mutants studied. For comparison between the potential lines (PL) and the control, a maximum similarity value (0.814) was observed in PL1 mutant, while the minimum value (0.537) was observed in PL7. In summary, a combination of irradiation, regeneration, multiplication, and random amplification of polymorphic DNA (RAPD) analysis for early screening of mutants can speed up the breeding program of E. elatior. Scientific and Technological Research Council of Turkey 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/78033/1/In%20vitro%20mutagenesis%20of%20Etlingera%20elatior%20%28Jack%29.pdf Yunus, Muhamad Fahmi and Abd Aziz, Maheran and Abdul Kadir, Mihdzar and Daud, Siti Khalijah and Abdul Rashid, Azmi (2013) In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers. Turkish Journal of Biology, 37 (6). pp. 716-725. ISSN 1300-0152 E-ISSN 1303-6092 http://journals.tubitak.gov.tr/biology/issues/biy-13-37-6/biy-37-6-11-1303-19.pdf 10.3906/biy-1303-19
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	SB Plant culture
spellingShingle	SB Plant culture Yunus, Muhamad Fahmi Abd Aziz, Maheran Abdul Kadir, Mihdzar Daud, Siti Khalijah Abdul Rashid, Azmi In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers
description	Mutation breeding techniques in combination with tissue culture and molecular marker methods provide a powerful tool for improvement of vegetatively propagated plants. The aim of this study was to develop a protocol for shoot regeneration and mutation induction of Etlingera elatior. The results of irradiation on in vitro buds of E. elatior showed LD50 to be 10 Gy, with the survival of explants being sharply reduced at this dosage. All 8 selected gamma irradiated regenerants were differentiated from the untreated control based on the banding patterns obtained using 9 primers, which generated 59 reproducible bands, whereby 35 (55.31%) were found to be polymorphic. Jaccard’s coefficient of similarity values ranging from 0.537 to 0.860 were indicative of the level of genetic variation among the mutants studied. For comparison between the potential lines (PL) and the control, a maximum similarity value (0.814) was observed in PL1 mutant, while the minimum value (0.537) was observed in PL7. In summary, a combination of irradiation, regeneration, multiplication, and random amplification of polymorphic DNA (RAPD) analysis for early screening of mutants can speed up the breeding program of E. elatior.
format	Article
author	Yunus, Muhamad Fahmi Abd Aziz, Maheran Abdul Kadir, Mihdzar Daud, Siti Khalijah Abdul Rashid, Azmi
author_facet	Yunus, Muhamad Fahmi Abd Aziz, Maheran Abdul Kadir, Mihdzar Daud, Siti Khalijah Abdul Rashid, Azmi
author_sort	Yunus, Muhamad Fahmi
title	In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers
title_short	In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers
title_full	In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers
title_fullStr	In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers
title_full_unstemmed	In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers
title_sort	in vitro mutagenesis of etlingera elatior (jack) and early detection of mutation using rapd markers
publisher	Scientific and Technological Research Council of Turkey
publishDate	2013
url	http://irep.iium.edu.my/78033/ http://irep.iium.edu.my/78033/ http://irep.iium.edu.my/78033/ http://irep.iium.edu.my/78033/1/In%20vitro%20mutagenesis%20of%20Etlingera%20elatior%20%28Jack%29.pdf
first_indexed	2023-09-18T21:49:59Z
last_indexed	2023-09-18T21:49:59Z
_version_	1777413704787165184

In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers

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