Assessment of genetic relationships within Bouea (Anacardiaceae) accessions in Peninsular Malaysia using inter simple sequence repeats (ISSR) markers

Malaysia is a genetically diverse hotspot for wild relatives of Mangifera and other Anacardiaceae members. The genus Bouea is interesting in that it has small sweet-sour edible fruits. This genus consists of two species, namely Kundang (Bouea macrophylla) and Remia (B. oppositifolia) which are of ec...

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Main Authors:	Ghazali, Mohd Norfaizal, Yunus, Muhamad Fahmi, Mohammad, Abdul Latiff
Format:	Article
Language:	English
Published:	African Journal of Biotechnology 2015
Subjects:	QK Botany SB Plant culture
Online Access:	http://irep.iium.edu.my/78036/ http://irep.iium.edu.my/78036/ http://irep.iium.edu.my/78036/ http://irep.iium.edu.my/78036/1/Assessment%20of%20genetic%20relationships%20within%20Bouea%20%28Anacardiaceae%29.pdf

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recordtype	eprints
spelling	iium-780362020-02-18T01:41:39Z http://irep.iium.edu.my/78036/ Assessment of genetic relationships within Bouea (Anacardiaceae) accessions in Peninsular Malaysia using inter simple sequence repeats (ISSR) markers Ghazali, Mohd Norfaizal Yunus, Muhamad Fahmi Mohammad, Abdul Latiff QK Botany SB Plant culture Malaysia is a genetically diverse hotspot for wild relatives of Mangifera and other Anacardiaceae members. The genus Bouea is interesting in that it has small sweet-sour edible fruits. This genus consists of two species, namely Kundang (Bouea macrophylla) and Remia (B. oppositifolia) which are of economic value. In this study, we highlighted the molecular evidences using inter simple sequence repeats (ISSR) markers to understand its genetic relatedness among accessions. A total of 52 ISSR have been screened on selected accession of Kundang and Remia from Peninsular Malaysia. Ten of the 52 primers for Kundang generated 89 scorable bands, where 45 bands (50.2%) were polymorphic. The simple matching coefficient of similarity provided similarity values ranging from 0.659 to 0.955 while relationship among the different accessions was distributed among three main divergent clusters. For Remia, 10 of the 52 primers tested gave 89 scorable bands, where 54 bands (59.5%) were polymorphic. The simple matching coefficient of similarity values ranged from 0.591 to 0.977 while the cluster analysis separated 23 accessions into two well-defined clusters. These studies indicate that there was considerable ISSR variation among the accessions reflecting variation among the accessions, which were morphologically indistinguishable. DNA polymorphism detected by ISSR analysis offers a useful molecular marker for the identification of different accessions of Kundang and Remia. African Journal of Biotechnology 2015-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/78036/1/Assessment%20of%20genetic%20relationships%20within%20Bouea%20%28Anacardiaceae%29.pdf Ghazali, Mohd Norfaizal and Yunus, Muhamad Fahmi and Mohammad, Abdul Latiff (2015) Assessment of genetic relationships within Bouea (Anacardiaceae) accessions in Peninsular Malaysia using inter simple sequence repeats (ISSR) markers. African Journal of Biotechnology, 14 (2). pp. 76-85. ISSN 1684-5315 https://www.ajol.info/index.php/ajb/article/view/115579/105151 10.5897/AJB2014.13971
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QK Botany SB Plant culture
spellingShingle	QK Botany SB Plant culture Ghazali, Mohd Norfaizal Yunus, Muhamad Fahmi Mohammad, Abdul Latiff Assessment of genetic relationships within Bouea (Anacardiaceae) accessions in Peninsular Malaysia using inter simple sequence repeats (ISSR) markers
description	Malaysia is a genetically diverse hotspot for wild relatives of Mangifera and other Anacardiaceae members. The genus Bouea is interesting in that it has small sweet-sour edible fruits. This genus consists of two species, namely Kundang (Bouea macrophylla) and Remia (B. oppositifolia) which are of economic value. In this study, we highlighted the molecular evidences using inter simple sequence repeats (ISSR) markers to understand its genetic relatedness among accessions. A total of 52 ISSR have been screened on selected accession of Kundang and Remia from Peninsular Malaysia. Ten of the 52 primers for Kundang generated 89 scorable bands, where 45 bands (50.2%) were polymorphic. The simple matching coefficient of similarity provided similarity values ranging from 0.659 to 0.955 while relationship among the different accessions was distributed among three main divergent clusters. For Remia, 10 of the 52 primers tested gave 89 scorable bands, where 54 bands (59.5%) were polymorphic. The simple matching coefficient of similarity values ranged from 0.591 to 0.977 while the cluster analysis separated 23 accessions into two well-defined clusters. These studies indicate that there was considerable ISSR variation among the accessions reflecting variation among the accessions, which were morphologically indistinguishable. DNA polymorphism detected by ISSR analysis offers a useful molecular marker for the identification of different accessions of Kundang and Remia.
format	Article
author	Ghazali, Mohd Norfaizal Yunus, Muhamad Fahmi Mohammad, Abdul Latiff
author_facet	Ghazali, Mohd Norfaizal Yunus, Muhamad Fahmi Mohammad, Abdul Latiff
author_sort	Ghazali, Mohd Norfaizal
title	Assessment of genetic relationships within Bouea (Anacardiaceae) accessions in Peninsular Malaysia using inter simple sequence repeats (ISSR) markers
title_short	Assessment of genetic relationships within Bouea (Anacardiaceae) accessions in Peninsular Malaysia using inter simple sequence repeats (ISSR) markers
title_full	Assessment of genetic relationships within Bouea (Anacardiaceae) accessions in Peninsular Malaysia using inter simple sequence repeats (ISSR) markers
title_fullStr	Assessment of genetic relationships within Bouea (Anacardiaceae) accessions in Peninsular Malaysia using inter simple sequence repeats (ISSR) markers
title_full_unstemmed	Assessment of genetic relationships within Bouea (Anacardiaceae) accessions in Peninsular Malaysia using inter simple sequence repeats (ISSR) markers
title_sort	assessment of genetic relationships within bouea (anacardiaceae) accessions in peninsular malaysia using inter simple sequence repeats (issr) markers
publisher	African Journal of Biotechnology
publishDate	2015
url	http://irep.iium.edu.my/78036/ http://irep.iium.edu.my/78036/ http://irep.iium.edu.my/78036/ http://irep.iium.edu.my/78036/1/Assessment%20of%20genetic%20relationships%20within%20Bouea%20%28Anacardiaceae%29.pdf
first_indexed	2023-09-18T21:50:00Z
last_indexed	2023-09-18T21:50:00Z
_version_	1777413705093349376

Assessment of genetic relationships within Bouea (Anacardiaceae) accessions in Peninsular Malaysia using inter simple sequence repeats (ISSR) markers

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