Effects of skin abrasion in immersion challenge with Vibrio harveyi in Asian seabass Lates calcarifer fingerlings

Skin abrasions often occur in farmed fish following handling by labourers, injury by farm facilities, cannibalism and ectoparasites. Vibrio spp. are opportunistic pathogens that can invade host fish through damaged tissues and cause outbreaks of vibriosis. This study describes the effect of skin abr...

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Main Authors:	Chin, Yong Kit, Md Yasin, Ina‐Salwany, M, Zamri-Saad, Al-saari, Nurhidayu
Format:	Article
Language:	English
Published:	2020
Subjects:	QR Microbiology SH151 Aquaculture - Fish Culture
Online Access:	http://irep.iium.edu.my/78159/ http://irep.iium.edu.my/78159/1/d137p167%20%281%29.pdf

id	iium-78159
recordtype	eprints
spelling	iium-781592020-01-21T08:18:20Z http://irep.iium.edu.my/78159/ Effects of skin abrasion in immersion challenge with Vibrio harveyi in Asian seabass Lates calcarifer fingerlings Chin, Yong Kit Md Yasin, Ina‐Salwany M, Zamri-Saad Al-saari, Nurhidayu QR Microbiology SH151 Aquaculture - Fish Culture Skin abrasions often occur in farmed fish following handling by labourers, injury by farm facilities, cannibalism and ectoparasites. Vibrio spp. are opportunistic pathogens that can invade host fish through damaged tissues and cause outbreaks of vibriosis. This study describes the effect of skin abrasions on the infectivity of V. harveyi using Asian seabass Lates calcarifer (Bloch, 1790) fingerlings as a case example and compares bacterial load and fish survival following immersion challenge with different doses. In total, 315 fish (6.67 ± 1.8 g) were divided into 3 treatments: skin abrasion followed by immersion infection, immersion infection only and an uninfected, uninjured control. Fish in the infection treatments were divided into 3 subgroups and exposed in triplicate to a 7 d immersion challenge with 106, 107 and 108 CFU ml−1 of live V. harveyi. No mortalities were observed in the control and immersion infection groups. However, fish in the skin abrasion treatment group that were infected with 108 CFU ml−1 of live V. harveyi showed signs of progressing disease throughout the experiment, which resulted in mortalities. Significantly higher bacterial loads (p < 0.05) were recorded in the intestine, liver and gills of the fish in this group. Fish in the skin abrasion treatment that were exposed to 107 and 108 CFU ml−1 of V. harveyi showed 100% mortality by Days 5 and 4, respectively. These findings confirm that skin injuries increase the susceptibility of seabass fingerlings to V. harveyi infection. 2020-01-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/78159/1/d137p167%20%281%29.pdf Chin, Yong Kit and Md Yasin, Ina‐Salwany and M, Zamri-Saad and Al-saari, Nurhidayu (2020) Effects of skin abrasion in immersion challenge with Vibrio harveyi in Asian seabass Lates calcarifer fingerlings. DISEASES OF AQUATIC ORGANISMS.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology SH151 Aquaculture - Fish Culture
spellingShingle	QR Microbiology SH151 Aquaculture - Fish Culture Chin, Yong Kit Md Yasin, Ina‐Salwany M, Zamri-Saad Al-saari, Nurhidayu Effects of skin abrasion in immersion challenge with Vibrio harveyi in Asian seabass Lates calcarifer fingerlings
description	Skin abrasions often occur in farmed fish following handling by labourers, injury by farm facilities, cannibalism and ectoparasites. Vibrio spp. are opportunistic pathogens that can invade host fish through damaged tissues and cause outbreaks of vibriosis. This study describes the effect of skin abrasions on the infectivity of V. harveyi using Asian seabass Lates calcarifer (Bloch, 1790) fingerlings as a case example and compares bacterial load and fish survival following immersion challenge with different doses. In total, 315 fish (6.67 ± 1.8 g) were divided into 3 treatments: skin abrasion followed by immersion infection, immersion infection only and an uninfected, uninjured control. Fish in the infection treatments were divided into 3 subgroups and exposed in triplicate to a 7 d immersion challenge with 106, 107 and 108 CFU ml−1 of live V. harveyi. No mortalities were observed in the control and immersion infection groups. However, fish in the skin abrasion treatment group that were infected with 108 CFU ml−1 of live V. harveyi showed signs of progressing disease throughout the experiment, which resulted in mortalities. Significantly higher bacterial loads (p < 0.05) were recorded in the intestine, liver and gills of the fish in this group. Fish in the skin abrasion treatment that were exposed to 107 and 108 CFU ml−1 of V. harveyi showed 100% mortality by Days 5 and 4, respectively. These findings confirm that skin injuries increase the susceptibility of seabass fingerlings to V. harveyi infection.
format	Article
author	Chin, Yong Kit Md Yasin, Ina‐Salwany M, Zamri-Saad Al-saari, Nurhidayu
author_facet	Chin, Yong Kit Md Yasin, Ina‐Salwany M, Zamri-Saad Al-saari, Nurhidayu
author_sort	Chin, Yong Kit
title	Effects of skin abrasion in immersion challenge with Vibrio harveyi in Asian seabass Lates calcarifer fingerlings
title_short	Effects of skin abrasion in immersion challenge with Vibrio harveyi in Asian seabass Lates calcarifer fingerlings
title_full	Effects of skin abrasion in immersion challenge with Vibrio harveyi in Asian seabass Lates calcarifer fingerlings
title_fullStr	Effects of skin abrasion in immersion challenge with Vibrio harveyi in Asian seabass Lates calcarifer fingerlings
title_full_unstemmed	Effects of skin abrasion in immersion challenge with Vibrio harveyi in Asian seabass Lates calcarifer fingerlings
title_sort	effects of skin abrasion in immersion challenge with vibrio harveyi in asian seabass lates calcarifer fingerlings
publishDate	2020
url	http://irep.iium.edu.my/78159/ http://irep.iium.edu.my/78159/1/d137p167%20%281%29.pdf
first_indexed	2023-09-18T21:50:09Z
last_indexed	2023-09-18T21:50:09Z
_version_	1777413715163873280

Effects of skin abrasion in immersion challenge with Vibrio harveyi in Asian seabass Lates calcarifer fingerlings

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