Effect of salicylic acid on carotenoids and chlorophyll content in Mas Cotek (Ficus Deltoidea Jack Var. Trengganuensis) leaves and its Retinol Activity Equivalents (RAE)
This study was conducted to determine the effect of different concentrations of salicylic acid (SA) (control, 0.01, 0.10, 1.00 mM) on carotenoids and chlorophyll content and its retinol activity equivalents (RAE) value in Ficus deltoidea Jack var. trengganuensis leaves. In this study, 12 seedlings...
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iium-784942020-02-06T02:12:56Z http://irep.iium.edu.my/78494/ Effect of salicylic acid on carotenoids and chlorophyll content in Mas Cotek (Ficus Deltoidea Jack Var. Trengganuensis) leaves and its Retinol Activity Equivalents (RAE) ISMAIL, Azrina Shahidan, Norshazila Mat, Nashriyah Othman, Rashidi RS Pharmacy and materia medica S Agriculture (General) SB Plant culture SD Forestry TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc This study was conducted to determine the effect of different concentrations of salicylic acid (SA) (control, 0.01, 0.10, 1.00 mM) on carotenoids and chlorophyll content and its retinol activity equivalents (RAE) value in Ficus deltoidea Jack var. trengganuensis leaves. In this study, 12 seedlings of Ficus deltoidea Jack var. trengganuensis were sprayed with different concentrations of SA. Carotenoid content was determined using High-Performance Liquid Chromatography (HPLC) and chlorophyll content was determined using chlorophyll meter. Retinol activity equivalents were calculated using RAE formulation. From the results obtained, two types of carotenoids, lutein and β-carotene, were detected in HPLC. The highest lutein and β-carotene content present in Ficus deltoidea Jack var. trengganuensis leaves extract was found in 0.10 mM SA treatment (93.50b ± 0.71 μg/g DW) for lutein and (282.00b ± 46.67 μg/g DW) for β-carotene, while the lowest lutein compound was found in 1.00 mM SA treatment (30.25a ± 1.77 μg/g DW) while the least β-carotenoid content was found in (1.00 mM SA) treatment (63.00a ± 0.71 μg/g DW). The same goes for retinol activity equivalents, the highest retinol activity equivalents was recorded in 0.10 mM SA treatment while lowest in 1.00 mM SA treatment. For chlorophyll content, the highest reading was showed in 0.10 mM SA treatment (73.50b ± 0.71 μg/g DW) while the lowest reading was in 1.00mM SA treatment (42.10a ± 1.41 μg/g DW). From this study, it can be concluded that salicylic acid at a certain concentration could increase or improve the carotenoid or chlorophyll content. Thus, it could be an alternative source of carotenoid and chlorophyll for the food and pharmaceutical industry in the future. SET PUBLISHER 2020-02-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/78494/1/JPANSV10N1A5-Shahidan.pdf ISMAIL, Azrina and Shahidan, Norshazila and Mat, Nashriyah and Othman, Rashidi (2020) Effect of salicylic acid on carotenoids and chlorophyll content in Mas Cotek (Ficus Deltoidea Jack Var. Trengganuensis) leaves and its Retinol Activity Equivalents (RAE). Journal of Pharmacy and Nutrition Sciences, 10 (1). pp. 25-33. ISSN 2223-3806 E-ISSN 1927-5951 |
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RS Pharmacy and materia medica S Agriculture (General) SB Plant culture SD Forestry TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc |
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RS Pharmacy and materia medica S Agriculture (General) SB Plant culture SD Forestry TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc ISMAIL, Azrina Shahidan, Norshazila Mat, Nashriyah Othman, Rashidi Effect of salicylic acid on carotenoids and chlorophyll content in Mas Cotek (Ficus Deltoidea Jack Var. Trengganuensis) leaves and its Retinol Activity Equivalents (RAE) |
description |
This study was conducted to determine the effect of different concentrations of salicylic acid (SA) (control,
0.01, 0.10, 1.00 mM) on carotenoids and chlorophyll content and its retinol activity equivalents (RAE) value in Ficus
deltoidea Jack var. trengganuensis leaves. In this study, 12 seedlings of Ficus deltoidea Jack var. trengganuensis were
sprayed with different concentrations of SA. Carotenoid content was determined using High-Performance Liquid
Chromatography (HPLC) and chlorophyll content was determined using chlorophyll meter. Retinol activity equivalents were calculated using RAE formulation. From the results obtained, two types of carotenoids, lutein and β-carotene, were detected in HPLC. The highest lutein and β-carotene content present in Ficus deltoidea Jack var. trengganuensis leaves extract was found in 0.10 mM SA treatment (93.50b ± 0.71 μg/g DW) for lutein and (282.00b ± 46.67 μg/g DW) for β-carotene, while the lowest lutein compound was found in 1.00 mM SA treatment (30.25a ± 1.77 μg/g DW) while the least β-carotenoid content was found in (1.00 mM SA) treatment (63.00a ± 0.71 μg/g DW). The same goes for retinol activity equivalents, the highest retinol activity equivalents was recorded in 0.10 mM SA treatment while lowest in 1.00 mM SA treatment. For chlorophyll content, the highest reading was showed in 0.10 mM SA treatment (73.50b ± 0.71 μg/g DW) while the lowest reading was in 1.00mM SA treatment (42.10a ± 1.41 μg/g DW). From this study, it can be concluded that salicylic acid at a certain concentration could increase or improve the carotenoid or chlorophyll content. Thus, it could be an alternative source of carotenoid and chlorophyll for the food and pharmaceutical industry in the future. |
format |
Article |
author |
ISMAIL, Azrina Shahidan, Norshazila Mat, Nashriyah Othman, Rashidi |
author_facet |
ISMAIL, Azrina Shahidan, Norshazila Mat, Nashriyah Othman, Rashidi |
author_sort |
ISMAIL, Azrina |
title |
Effect of salicylic acid on carotenoids and chlorophyll content in Mas Cotek (Ficus Deltoidea Jack Var. Trengganuensis) leaves and its Retinol Activity Equivalents (RAE) |
title_short |
Effect of salicylic acid on carotenoids and chlorophyll content in Mas Cotek (Ficus Deltoidea Jack Var. Trengganuensis) leaves and its Retinol Activity Equivalents (RAE) |
title_full |
Effect of salicylic acid on carotenoids and chlorophyll content in Mas Cotek (Ficus Deltoidea Jack Var. Trengganuensis) leaves and its Retinol Activity Equivalents (RAE) |
title_fullStr |
Effect of salicylic acid on carotenoids and chlorophyll content in Mas Cotek (Ficus Deltoidea Jack Var. Trengganuensis) leaves and its Retinol Activity Equivalents (RAE) |
title_full_unstemmed |
Effect of salicylic acid on carotenoids and chlorophyll content in Mas Cotek (Ficus Deltoidea Jack Var. Trengganuensis) leaves and its Retinol Activity Equivalents (RAE) |
title_sort |
effect of salicylic acid on carotenoids and chlorophyll content in mas cotek (ficus deltoidea jack var. trengganuensis) leaves and its retinol activity equivalents (rae) |
publisher |
SET PUBLISHER |
publishDate |
2020 |
url |
http://irep.iium.edu.my/78494/ http://irep.iium.edu.my/78494/1/JPANSV10N1A5-Shahidan.pdf |
first_indexed |
2023-09-18T21:50:35Z |
last_indexed |
2023-09-18T21:50:35Z |
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1777413742614544384 |