Backward extraction of jacalin-loaded reverse micelles into new stripping phase and toxicity evaluation on embryonic development of zebrafish

This study demonstrates the effects of aqueous phase pH, KCl concentration and IPA amount on backward extraction efficiency of jacalin from AOT reverse micellar phase into a fresh stripping phase solution. The best condition for backward extraction of jacalin was achieved at the stripping phase pH o...

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Main Authors:	Sy Mohamad, Sharifah Fathiyah, Mohd Said, Farhan, Abdul Munaim, Mimi Sakinah, Mohamad, Shahril, Wan Sulaiman, Wan Mohd. Azizi
Format:	Article
Language:	English English
Published:	Malaysian Society for Biochemistry and Molecular Biology 2019
Subjects:	QL Zoology
Online Access:	http://irep.iium.edu.my/79079/ http://irep.iium.edu.my/79079/ http://irep.iium.edu.my/79079/1/79079_Backward%20extraction%20of%20jacalin-loaded.pdf http://irep.iium.edu.my/79079/2/79079_Backward%20extraction%20of%20jacalin-loaded_SCOPUS.pdf

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recordtype	eprints
spelling	iium-790792020-02-25T00:52:42Z http://irep.iium.edu.my/79079/ Backward extraction of jacalin-loaded reverse micelles into new stripping phase and toxicity evaluation on embryonic development of zebrafish Sy Mohamad, Sharifah Fathiyah Mohd Said, Farhan Abdul Munaim, Mimi Sakinah Mohamad, Shahril Wan Sulaiman, Wan Mohd. Azizi QL Zoology This study demonstrates the effects of aqueous phase pH, KCl concentration and IPA amount on backward extraction efficiency of jacalin from AOT reverse micellar phase into a fresh stripping phase solution. The best condition for backward extraction of jacalin was achieved at the stripping phase pH of 9, 0.75 M KCl and with the addition of 30% v/v IPA. This work also investigates the possible toxicity caused by the backward-extracted jacalin in vivo using zebrafish embryo as a model organism. Our results showed that within the tested concentrations, the purified jacalin had a potentially harmful effect on the embryonic development of zebrafish embryos, as demonstrated by the surviving rates and morphological examination. The survival rate of embryos was found to decrease in concentration and time-dependent manner. The computed LC50 were 20.35, 18.86 and 14.81 µg/ml after exposures of 48, 72 and 96 hours, respectively. Major morphological malformations observed in jacalin-treated embryos were pericardial edema and body curvature for the concentration of jacalin above 12.5 µg/ml. Hence, it can be concluded that the reverse micellar extraction is a suitable method for purification of jacalin from jackfruit seeds, the use of jacalin for therapeutic applications, however, should be done with caution Malaysian Society for Biochemistry and Molecular Biology 2019-03-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/79079/1/79079_Backward%20extraction%20of%20jacalin-loaded.pdf application/pdf en http://irep.iium.edu.my/79079/2/79079_Backward%20extraction%20of%20jacalin-loaded_SCOPUS.pdf Sy Mohamad, Sharifah Fathiyah and Mohd Said, Farhan and Abdul Munaim, Mimi Sakinah and Mohamad, Shahril and Wan Sulaiman, Wan Mohd. Azizi (2019) Backward extraction of jacalin-loaded reverse micelles into new stripping phase and toxicity evaluation on embryonic development of zebrafish. Malaysian Journal of Biochemistry and Molecular Biology, 22 (1). pp. 134-141. ISSN 15112616 https://msbmb2010.wixsite.com/mjbmb/copy-of-home-5
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QL Zoology
spellingShingle	QL Zoology Sy Mohamad, Sharifah Fathiyah Mohd Said, Farhan Abdul Munaim, Mimi Sakinah Mohamad, Shahril Wan Sulaiman, Wan Mohd. Azizi Backward extraction of jacalin-loaded reverse micelles into new stripping phase and toxicity evaluation on embryonic development of zebrafish
description	This study demonstrates the effects of aqueous phase pH, KCl concentration and IPA amount on backward extraction efficiency of jacalin from AOT reverse micellar phase into a fresh stripping phase solution. The best condition for backward extraction of jacalin was achieved at the stripping phase pH of 9, 0.75 M KCl and with the addition of 30% v/v IPA. This work also investigates the possible toxicity caused by the backward-extracted jacalin in vivo using zebrafish embryo as a model organism. Our results showed that within the tested concentrations, the purified jacalin had a potentially harmful effect on the embryonic development of zebrafish embryos, as demonstrated by the surviving rates and morphological examination. The survival rate of embryos was found to decrease in concentration and time-dependent manner. The computed LC50 were 20.35, 18.86 and 14.81 µg/ml after exposures of 48, 72 and 96 hours, respectively. Major morphological malformations observed in jacalin-treated embryos were pericardial edema and body curvature for the concentration of jacalin above 12.5 µg/ml. Hence, it can be concluded that the reverse micellar extraction is a suitable method for purification of jacalin from jackfruit seeds, the use of jacalin for therapeutic applications, however, should be done with caution
format	Article
author	Sy Mohamad, Sharifah Fathiyah Mohd Said, Farhan Abdul Munaim, Mimi Sakinah Mohamad, Shahril Wan Sulaiman, Wan Mohd. Azizi
author_facet	Sy Mohamad, Sharifah Fathiyah Mohd Said, Farhan Abdul Munaim, Mimi Sakinah Mohamad, Shahril Wan Sulaiman, Wan Mohd. Azizi
author_sort	Sy Mohamad, Sharifah Fathiyah
title	Backward extraction of jacalin-loaded reverse micelles into new stripping phase and toxicity evaluation on embryonic development of zebrafish
title_short	Backward extraction of jacalin-loaded reverse micelles into new stripping phase and toxicity evaluation on embryonic development of zebrafish
title_full	Backward extraction of jacalin-loaded reverse micelles into new stripping phase and toxicity evaluation on embryonic development of zebrafish
title_fullStr	Backward extraction of jacalin-loaded reverse micelles into new stripping phase and toxicity evaluation on embryonic development of zebrafish
title_full_unstemmed	Backward extraction of jacalin-loaded reverse micelles into new stripping phase and toxicity evaluation on embryonic development of zebrafish
title_sort	backward extraction of jacalin-loaded reverse micelles into new stripping phase and toxicity evaluation on embryonic development of zebrafish
publisher	Malaysian Society for Biochemistry and Molecular Biology
publishDate	2019
url	http://irep.iium.edu.my/79079/ http://irep.iium.edu.my/79079/ http://irep.iium.edu.my/79079/1/79079_Backward%20extraction%20of%20jacalin-loaded.pdf http://irep.iium.edu.my/79079/2/79079_Backward%20extraction%20of%20jacalin-loaded_SCOPUS.pdf
first_indexed	2023-09-18T21:51:15Z
last_indexed	2023-09-18T21:51:15Z
_version_	1777413783653711872

Backward extraction of jacalin-loaded reverse micelles into new stripping phase and toxicity evaluation on embryonic development of zebrafish

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