Variation in the metabolites and αglucosidase inhibitory activity of Cosmos caudatus at different growth stages

Background: Cosmos caudatus is an annual plant known for its medicinal value in treating several health conditions, such as high blood pressure, arthritis, and diabetes mellitus. The α-glucosidase inhibitory activity and total phenolic content of the leaf aqueous ethanolic extracts of the plant at d...

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Main Authors:	Wan Ahmad, Wan Nadilah, Akhtar, Muhammad Tayyab, Shaari, Khozirah, Khatib, Alfi, Abdul Hamid, Azizah, Hamid, Muhajir
Format:	Article
Language:	English English English
Published:	NLM (Medline) 2019
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/79132/ http://irep.iium.edu.my/79132/ http://irep.iium.edu.my/79132/ http://irep.iium.edu.my/79132/1/Wan%20Nadilah%202019%2C%20BMC%20complementary.pdf http://irep.iium.edu.my/79132/7/Scopus%20-%20cosmos%20caudatus.pdf http://irep.iium.edu.my/79132/13/79132_Variation%20in%20the%20metabolites_wos.pdf

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spelling	iium-791322020-03-18T01:56:42Z http://irep.iium.edu.my/79132/ Variation in the metabolites and αglucosidase inhibitory activity of Cosmos caudatus at different growth stages Wan Ahmad, Wan Nadilah Akhtar, Muhammad Tayyab Shaari, Khozirah Khatib, Alfi Abdul Hamid, Azizah Hamid, Muhajir Q Science (General) Background: Cosmos caudatus is an annual plant known for its medicinal value in treating several health conditions, such as high blood pressure, arthritis, and diabetes mellitus. The α-glucosidase inhibitory activity and total phenolic content of the leaf aqueous ethanolic extracts of the plant at different growth stages (6, 8. 10, 12 and 14weeks) were determined in an effort to ascertain the best time to harvest the plant for maximum medicinal quality with respect to its glucose-lowering effects. Methods: The aqueous ethanolic leaf extracts of C. caudatus were characterized by NMR and LC-MS/MS. The total phenolic content and α-glucosidase inhibitory activity were evaluated by the Folin-Ciocalteu method and α-glucosidase inhibitory assay, respectively. The statistical significance of the results was evaluated using one-way ANOVA with Duncan’s post hoc test, and correlation among the different activities was performed by Pearson’s correlation test. NMR spectroscopy along with multivariate data analysis was used to identify the metabolites correlated with total phenolic content and α-glucosidase inhibitory activity of the C. caudatus leaf extracts. Results: It was found that the α-glucosidase inhibitory activity and total phenolic content of the optimized ethanol: water (80:20) leaf extract of the plant increased significantly as the plant matured, reaching a maximum at the 10th week. The IC50 value for α-glucosidase inhibitory activity (39.18μgmL− 1) at the 10th week showed greater potency than the positive standard, quercetin (110.50μgmL− 1). Through an 1H NMR-based metabolomics approach, the 10-week-old samples were shown to be correlated with a high total phenolic content and α-glucosidase inhibitory activity. From the partial least squares biplot, rutin and flavonoid glycosides, consisting of quercetin 3-Oarabinofuranoside, quercetin 3-O-rhamnoside, quercetin 3-O-glucoside, and quercetin 3-O-xyloside, were identified as the major bioactive metabolites. The metabolites were identified by NMR spectroscopy (J-resolve, HSQC and HMBC experiments) and further supported by dereplication via LC-MS/MS. Conclusion: For high phytomedicinal quality, the 10th week is recommended as the best time to harvest C. caudatus leaves with respect to its glucose lowering potential. NLM (Medline) 2019-05-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/79132/1/Wan%20Nadilah%202019%2C%20BMC%20complementary.pdf application/pdf en http://irep.iium.edu.my/79132/7/Scopus%20-%20cosmos%20caudatus.pdf application/pdf en http://irep.iium.edu.my/79132/13/79132_Variation%20in%20the%20metabolites_wos.pdf Wan Ahmad, Wan Nadilah and Akhtar, Muhammad Tayyab and Shaari, Khozirah and Khatib, Alfi and Abdul Hamid, Azizah and Hamid, Muhajir (2019) Variation in the metabolites and αglucosidase inhibitory activity of Cosmos caudatus at different growth stages. BMC Complementary And Alternative Medicine, 19 (1). pp. 1-15. ISSN 14726882 https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-019-2655-9 10.1186/s12906-019-2655-9
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	Q Science (General)
spellingShingle	Q Science (General) Wan Ahmad, Wan Nadilah Akhtar, Muhammad Tayyab Shaari, Khozirah Khatib, Alfi Abdul Hamid, Azizah Hamid, Muhajir Variation in the metabolites and αglucosidase inhibitory activity of Cosmos caudatus at different growth stages
description	Background: Cosmos caudatus is an annual plant known for its medicinal value in treating several health conditions, such as high blood pressure, arthritis, and diabetes mellitus. The α-glucosidase inhibitory activity and total phenolic content of the leaf aqueous ethanolic extracts of the plant at different growth stages (6, 8. 10, 12 and 14weeks) were determined in an effort to ascertain the best time to harvest the plant for maximum medicinal quality with respect to its glucose-lowering effects. Methods: The aqueous ethanolic leaf extracts of C. caudatus were characterized by NMR and LC-MS/MS. The total phenolic content and α-glucosidase inhibitory activity were evaluated by the Folin-Ciocalteu method and α-glucosidase inhibitory assay, respectively. The statistical significance of the results was evaluated using one-way ANOVA with Duncan’s post hoc test, and correlation among the different activities was performed by Pearson’s correlation test. NMR spectroscopy along with multivariate data analysis was used to identify the metabolites correlated with total phenolic content and α-glucosidase inhibitory activity of the C. caudatus leaf extracts. Results: It was found that the α-glucosidase inhibitory activity and total phenolic content of the optimized ethanol: water (80:20) leaf extract of the plant increased significantly as the plant matured, reaching a maximum at the 10th week. The IC50 value for α-glucosidase inhibitory activity (39.18μgmL− 1) at the 10th week showed greater potency than the positive standard, quercetin (110.50μgmL− 1). Through an 1H NMR-based metabolomics approach, the 10-week-old samples were shown to be correlated with a high total phenolic content and α-glucosidase inhibitory activity. From the partial least squares biplot, rutin and flavonoid glycosides, consisting of quercetin 3-Oarabinofuranoside, quercetin 3-O-rhamnoside, quercetin 3-O-glucoside, and quercetin 3-O-xyloside, were identified as the major bioactive metabolites. The metabolites were identified by NMR spectroscopy (J-resolve, HSQC and HMBC experiments) and further supported by dereplication via LC-MS/MS. Conclusion: For high phytomedicinal quality, the 10th week is recommended as the best time to harvest C. caudatus leaves with respect to its glucose lowering potential.
format	Article
author	Wan Ahmad, Wan Nadilah Akhtar, Muhammad Tayyab Shaari, Khozirah Khatib, Alfi Abdul Hamid, Azizah Hamid, Muhajir
author_facet	Wan Ahmad, Wan Nadilah Akhtar, Muhammad Tayyab Shaari, Khozirah Khatib, Alfi Abdul Hamid, Azizah Hamid, Muhajir
author_sort	Wan Ahmad, Wan Nadilah
title	Variation in the metabolites and αglucosidase inhibitory activity of Cosmos caudatus at different growth stages
title_short	Variation in the metabolites and αglucosidase inhibitory activity of Cosmos caudatus at different growth stages
title_full	Variation in the metabolites and αglucosidase inhibitory activity of Cosmos caudatus at different growth stages
title_fullStr	Variation in the metabolites and αglucosidase inhibitory activity of Cosmos caudatus at different growth stages
title_full_unstemmed	Variation in the metabolites and αglucosidase inhibitory activity of Cosmos caudatus at different growth stages
title_sort	variation in the metabolites and αglucosidase inhibitory activity of cosmos caudatus at different growth stages
publisher	NLM (Medline)
publishDate	2019
url	http://irep.iium.edu.my/79132/ http://irep.iium.edu.my/79132/ http://irep.iium.edu.my/79132/ http://irep.iium.edu.my/79132/1/Wan%20Nadilah%202019%2C%20BMC%20complementary.pdf http://irep.iium.edu.my/79132/7/Scopus%20-%20cosmos%20caudatus.pdf http://irep.iium.edu.my/79132/13/79132_Variation%20in%20the%20metabolites_wos.pdf
first_indexed	2023-09-18T21:51:16Z
last_indexed	2023-09-18T21:51:16Z
_version_	1777413785418465280

Variation in the metabolites and αglucosidase inhibitory activity of Cosmos caudatus at different growth stages

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