Discrimination of Clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting

Clinacanthus nutans or Sabah Snake Grass is a small shrub indigenous to tropical Asia. It has been used in the treatment of skin rashes, insect and snake bites, cancer and diabetes. In this study, Fourier transform infrared spectroscopy was used to discriminate the effects of different extraction me...

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Main Authors:	Noor Hashim, Noor Haslinda, Ali, Amatul Hamizah, Khatib, Alfi, Latip, Jalifah
Format:	Article
Language:	English English
Published:	Elsevier B.V. 2019
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/79147/ http://irep.iium.edu.my/79147/ http://irep.iium.edu.my/79147/ http://irep.iium.edu.my/79147/1/hashim%20et%20al%202019-vibspec.pdf http://irep.iium.edu.my/79147/7/Scopus%20-%20discrimination%20of%20clinacanthus.pdf

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spelling	iium-791472020-02-26T07:27:08Z http://irep.iium.edu.my/79147/ Discrimination of Clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting Noor Hashim, Noor Haslinda Ali, Amatul Hamizah Khatib, Alfi Latip, Jalifah Q Science (General) Clinacanthus nutans or Sabah Snake Grass is a small shrub indigenous to tropical Asia. It has been used in the treatment of skin rashes, insect and snake bites, cancer and diabetes. In this study, Fourier transform infrared spectroscopy was used to discriminate the effects of different extraction methods on the chemical composition of C. nutans freeze dried leaves and stems. The samples were extracted using different methods (soaking and sonication) followed by the measurement of its antiplasmodial activity. Based on the absorption peaks, C. nutans extracts comprise of compounds with hydroxyl, methyl, sulfoxide, sulfone, aromatic and carbonyl functionalities which indicate the presence of carbohydrates, terpenes, sulfurous glycosides and aromatic compounds in the extracts. Multivariate data analysis such as orthogonal partial least square (OPLS) was used to correlate the FTIR spectra of the extracts with antiplasmodial activity. The OPLS model exhibited R2Y and Q2Y values of 0.896 and 0.736, respectively. The RMSEE and RMSECV value were 22.41 and 32.36. The loading line plot of the OPLS model revealed that OH, C[dbnd]O, C[sbnd]H and C[sbnd]C functional group induced the activity, whereas, C[sbnd]N and S[dbnd]O reduced the bioactivity. These preliminary results demonstrated that FTIR spectroscopy can be used as a simple and rapid technique to discriminate C. nutans extracts obtained from different extraction methods which is useful in the quality control during processing of this plant. Elsevier B.V. 2019-09-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/79147/1/hashim%20et%20al%202019-vibspec.pdf application/pdf en http://irep.iium.edu.my/79147/7/Scopus%20-%20discrimination%20of%20clinacanthus.pdf Noor Hashim, Noor Haslinda and Ali, Amatul Hamizah and Khatib, Alfi and Latip, Jalifah (2019) Discrimination of Clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting. Vibrational Spectroscopy, 104. pp. 1-6. ISSN 092-42031 https://www.sciencedirect.com/science/article/abs/pii/S092420311930075X?via%3Dihub 10.1016/j.vibspec.2019.102966
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Noor Hashim, Noor Haslinda Ali, Amatul Hamizah Khatib, Alfi Latip, Jalifah Discrimination of Clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting
description	Clinacanthus nutans or Sabah Snake Grass is a small shrub indigenous to tropical Asia. It has been used in the treatment of skin rashes, insect and snake bites, cancer and diabetes. In this study, Fourier transform infrared spectroscopy was used to discriminate the effects of different extraction methods on the chemical composition of C. nutans freeze dried leaves and stems. The samples were extracted using different methods (soaking and sonication) followed by the measurement of its antiplasmodial activity. Based on the absorption peaks, C. nutans extracts comprise of compounds with hydroxyl, methyl, sulfoxide, sulfone, aromatic and carbonyl functionalities which indicate the presence of carbohydrates, terpenes, sulfurous glycosides and aromatic compounds in the extracts. Multivariate data analysis such as orthogonal partial least square (OPLS) was used to correlate the FTIR spectra of the extracts with antiplasmodial activity. The OPLS model exhibited R2Y and Q2Y values of 0.896 and 0.736, respectively. The RMSEE and RMSECV value were 22.41 and 32.36. The loading line plot of the OPLS model revealed that OH, C[dbnd]O, C[sbnd]H and C[sbnd]C functional group induced the activity, whereas, C[sbnd]N and S[dbnd]O reduced the bioactivity. These preliminary results demonstrated that FTIR spectroscopy can be used as a simple and rapid technique to discriminate C. nutans extracts obtained from different extraction methods which is useful in the quality control during processing of this plant.
format	Article
author	Noor Hashim, Noor Haslinda Ali, Amatul Hamizah Khatib, Alfi Latip, Jalifah
author_facet	Noor Hashim, Noor Haslinda Ali, Amatul Hamizah Khatib, Alfi Latip, Jalifah
author_sort	Noor Hashim, Noor Haslinda
title	Discrimination of Clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting
title_short	Discrimination of Clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting
title_full	Discrimination of Clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting
title_fullStr	Discrimination of Clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting
title_full_unstemmed	Discrimination of Clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting
title_sort	discrimination of clinacanthus nutans extracts and correlation with antiplasmodial activity using atr-ftir fingerprinting
publisher	Elsevier B.V.
publishDate	2019
url	http://irep.iium.edu.my/79147/ http://irep.iium.edu.my/79147/ http://irep.iium.edu.my/79147/ http://irep.iium.edu.my/79147/1/hashim%20et%20al%202019-vibspec.pdf http://irep.iium.edu.my/79147/7/Scopus%20-%20discrimination%20of%20clinacanthus.pdf
first_indexed	2023-09-18T21:51:17Z
last_indexed	2023-09-18T21:51:17Z
_version_	1777413786438729728

Discrimination of Clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting

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