Expression of microrna-101 in formalin-fixed paraffin- embedded samples of nasopharyngeal carcinoma

Nasopharyngeal carcinoma (NPC) is among the five most common malignancies in Malaysia. Most NPC patients are diagnosed at late stages of the disease which complicates the clinical management of the patients. Identification of new reliable biomarker is crucial to improve early diagnosis of NPC and in...

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Main Authors:	Ab. Wahid, Haziq, Othman, Noor Syamila, Ahmad, Azmir, Muhammad, Siti Aesah @ Naznin, Abdullah, Kahairi, Leman, Wan Ishlah, Mohd. Shiyuti, Muhammad Izani, Rosla, Luqman, Kaderi, Mohd Arifin
Format:	Article
Language:	English English
Published:	Malaysian Society for Biochemistry and Molecular Biology 2019
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/79672/ http://irep.iium.edu.my/79672/ http://irep.iium.edu.my/79672/1/79672_Expression%20of%20microrna-101.pdf http://irep.iium.edu.my/79672/2/79672_Expression%20of%20microrna-101_SCOPUS.pdf

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recordtype	eprints
spelling	iium-796722020-03-19T14:16:42Z http://irep.iium.edu.my/79672/ Expression of microrna-101 in formalin-fixed paraffin- embedded samples of nasopharyngeal carcinoma Ab. Wahid, Haziq Othman, Noor Syamila Ahmad, Azmir Muhammad, Siti Aesah @ Naznin Abdullah, Kahairi Leman, Wan Ishlah Mohd. Shiyuti, Muhammad Izani Rosla, Luqman Kaderi, Mohd Arifin QD Chemistry Nasopharyngeal carcinoma (NPC) is among the five most common malignancies in Malaysia. Most NPC patients are diagnosed at late stages of the disease which complicates the clinical management of the patients. Identification of new reliable biomarker is crucial to improve early diagnosis of NPC and increase the survival rate of patients. Recent study found that microRNAs (miRNAs), particularly miR-101, were involved in the tumorigenesis of head and neck cancer where NPC samples were included in the study. This study was conducted to observe the expression of miR-101 in NPC tumour tissues and compare its consistency with previous study as a step towards finding the new biomarker for NPC. The biopsy samples were obtained from hospitals and verified histologically using hematoxylin and eosin method for tissue classification. Total RNA was extracted from NPC tissues and normal nasopharyngeal epithelium tissues. The expression of miR-101 in NPC was quantified using quantitative polymerase chain reaction (qPCR) method. The differential expression of miR-101 in NPC as compared to normal nasopharyngeal epithelium tissues was analysed using 2-ΔΔCT calculation. The significance of the differential expression was analysed using SPSS software. Five samples have been verified as NPC and three samples were normal nasopharyngeal epithelium. The differential expression calculation found that miR-101 was downregulated in NPC as compared to normal nasopharyngeal epithelium tissues, which consistent with previous study. However, the differential expression was not significant. Therefore, our finding provides a preliminary result towards embarkment of a larger and comprehensive study. © 2019 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved. Malaysian Society for Biochemistry and Molecular Biology 2019-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/79672/1/79672_Expression%20of%20microrna-101.pdf application/pdf en http://irep.iium.edu.my/79672/2/79672_Expression%20of%20microrna-101_SCOPUS.pdf Ab. Wahid, Haziq and Othman, Noor Syamila and Ahmad, Azmir and Muhammad, Siti Aesah @ Naznin and Abdullah, Kahairi and Leman, Wan Ishlah and Mohd. Shiyuti, Muhammad Izani and Rosla, Luqman and Kaderi, Mohd Arifin (2019) Expression of microrna-101 in formalin-fixed paraffin- embedded samples of nasopharyngeal carcinoma. Malaysian Journal of Biochemistry and Molecular Biology, 22 (2). pp. 60-63. ISSN 1511-2616 https://1f169227-0328-4af2-99bc-24934f131be8.filesusr.com/ugd/66925b_f13e47f32fb641aba4d75ff3cb419b71.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Ab. Wahid, Haziq Othman, Noor Syamila Ahmad, Azmir Muhammad, Siti Aesah @ Naznin Abdullah, Kahairi Leman, Wan Ishlah Mohd. Shiyuti, Muhammad Izani Rosla, Luqman Kaderi, Mohd Arifin Expression of microrna-101 in formalin-fixed paraffin- embedded samples of nasopharyngeal carcinoma
description	Nasopharyngeal carcinoma (NPC) is among the five most common malignancies in Malaysia. Most NPC patients are diagnosed at late stages of the disease which complicates the clinical management of the patients. Identification of new reliable biomarker is crucial to improve early diagnosis of NPC and increase the survival rate of patients. Recent study found that microRNAs (miRNAs), particularly miR-101, were involved in the tumorigenesis of head and neck cancer where NPC samples were included in the study. This study was conducted to observe the expression of miR-101 in NPC tumour tissues and compare its consistency with previous study as a step towards finding the new biomarker for NPC. The biopsy samples were obtained from hospitals and verified histologically using hematoxylin and eosin method for tissue classification. Total RNA was extracted from NPC tissues and normal nasopharyngeal epithelium tissues. The expression of miR-101 in NPC was quantified using quantitative polymerase chain reaction (qPCR) method. The differential expression of miR-101 in NPC as compared to normal nasopharyngeal epithelium tissues was analysed using 2-ΔΔCT calculation. The significance of the differential expression was analysed using SPSS software. Five samples have been verified as NPC and three samples were normal nasopharyngeal epithelium. The differential expression calculation found that miR-101 was downregulated in NPC as compared to normal nasopharyngeal epithelium tissues, which consistent with previous study. However, the differential expression was not significant. Therefore, our finding provides a preliminary result towards embarkment of a larger and comprehensive study. © 2019 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.
format	Article
author	Ab. Wahid, Haziq Othman, Noor Syamila Ahmad, Azmir Muhammad, Siti Aesah @ Naznin Abdullah, Kahairi Leman, Wan Ishlah Mohd. Shiyuti, Muhammad Izani Rosla, Luqman Kaderi, Mohd Arifin
author_facet	Ab. Wahid, Haziq Othman, Noor Syamila Ahmad, Azmir Muhammad, Siti Aesah @ Naznin Abdullah, Kahairi Leman, Wan Ishlah Mohd. Shiyuti, Muhammad Izani Rosla, Luqman Kaderi, Mohd Arifin
author_sort	Ab. Wahid, Haziq
title	Expression of microrna-101 in formalin-fixed paraffin- embedded samples of nasopharyngeal carcinoma
title_short	Expression of microrna-101 in formalin-fixed paraffin- embedded samples of nasopharyngeal carcinoma
title_full	Expression of microrna-101 in formalin-fixed paraffin- embedded samples of nasopharyngeal carcinoma
title_fullStr	Expression of microrna-101 in formalin-fixed paraffin- embedded samples of nasopharyngeal carcinoma
title_full_unstemmed	Expression of microrna-101 in formalin-fixed paraffin- embedded samples of nasopharyngeal carcinoma
title_sort	expression of microrna-101 in formalin-fixed paraffin- embedded samples of nasopharyngeal carcinoma
publisher	Malaysian Society for Biochemistry and Molecular Biology
publishDate	2019
url	http://irep.iium.edu.my/79672/ http://irep.iium.edu.my/79672/ http://irep.iium.edu.my/79672/1/79672_Expression%20of%20microrna-101.pdf http://irep.iium.edu.my/79672/2/79672_Expression%20of%20microrna-101_SCOPUS.pdf
first_indexed	2023-09-18T21:51:40Z
last_indexed	2023-09-18T21:51:40Z
_version_	1777413810184781824

Expression of microrna-101 in formalin-fixed paraffin- embedded samples of nasopharyngeal carcinoma

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