Study of cells attachment using impedance spectroscopy technique

In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell–substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwell...

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Main Authors: Nordin, Anis Nurashikin, S.M., Arifuzzaman, Mel, Maizirwan, Spray, David, Voiculescu, Ioana
Format: Conference or Workshop Item
Language:English
Published: 2011
Subjects:
Online Access:http://irep.iium.edu.my/8147/
http://irep.iium.edu.my/8147/
http://irep.iium.edu.my/8147/1/IMECE2011-62919.pdf
id iium-8147
recordtype eprints
spelling iium-81472011-11-29T07:51:07Z http://irep.iium.edu.my/8147/ Study of cells attachment using impedance spectroscopy technique Nordin, Anis Nurashikin S.M., Arifuzzaman Mel, Maizirwan Spray, David Voiculescu, Ioana TA164 Bioengineering TP248.13 Biotechnology In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell–substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwell cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements were also simulated using COMSOL Multiphysics, a commercially available modeling package. 2011-11 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/8147/1/IMECE2011-62919.pdf Nordin, Anis Nurashikin and S.M., Arifuzzaman and Mel, Maizirwan and Spray, David and Voiculescu, Ioana (2011) Study of cells attachment using impedance spectroscopy technique. In: International Mechanical Engineering Congress & Exposition (IMECE 2011), 11-17 November 2011, Denver, Colorado. http://www.asmeconferences.org/Congress2011/
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TA164 Bioengineering
TP248.13 Biotechnology
spellingShingle TA164 Bioengineering
TP248.13 Biotechnology
Nordin, Anis Nurashikin
S.M., Arifuzzaman
Mel, Maizirwan
Spray, David
Voiculescu, Ioana
Study of cells attachment using impedance spectroscopy technique
description In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell–substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwell cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements were also simulated using COMSOL Multiphysics, a commercially available modeling package.
format Conference or Workshop Item
author Nordin, Anis Nurashikin
S.M., Arifuzzaman
Mel, Maizirwan
Spray, David
Voiculescu, Ioana
author_facet Nordin, Anis Nurashikin
S.M., Arifuzzaman
Mel, Maizirwan
Spray, David
Voiculescu, Ioana
author_sort Nordin, Anis Nurashikin
title Study of cells attachment using impedance spectroscopy technique
title_short Study of cells attachment using impedance spectroscopy technique
title_full Study of cells attachment using impedance spectroscopy technique
title_fullStr Study of cells attachment using impedance spectroscopy technique
title_full_unstemmed Study of cells attachment using impedance spectroscopy technique
title_sort study of cells attachment using impedance spectroscopy technique
publishDate 2011
url http://irep.iium.edu.my/8147/
http://irep.iium.edu.my/8147/
http://irep.iium.edu.my/8147/1/IMECE2011-62919.pdf
first_indexed 2023-09-18T20:17:48Z
last_indexed 2023-09-18T20:17:48Z
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