Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.

Researches concerning antioxidants are extensively studied nowadays. Antioxidants are well known to be associated with reduction in cancer incidence since they act against deteriorative effects of free radicals. These antioxidants include the yellow-to-red coloured pigments known as carotenoids, are...

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Main Authors:	Mohd. Zaifuddin, Fatimah Azzah, Mohd Hassan, Norazian, Othman, Rashidi
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	QK Botany SB Plant culture
Online Access:	http://irep.iium.edu.my/8278/ http://irep.iium.edu.my/8278/ http://irep.iium.edu.my/8278/1/POSTER.pdf

id	iium-8278
recordtype	eprints
spelling	iium-82782011-12-30T06:55:25Z http://irep.iium.edu.my/8278/ Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers. Mohd. Zaifuddin, Fatimah Azzah Mohd Hassan, Norazian Othman, Rashidi QK Botany SB Plant culture Researches concerning antioxidants are extensively studied nowadays. Antioxidants are well known to be associated with reduction in cancer incidence since they act against deteriorative effects of free radicals. These antioxidants include the yellow-to-red coloured pigments known as carotenoids, are essential for betterment in human immunity. Carotenoids can be found abundantly in dark green vegetables and also yellow, orange and red coloured fruits. They have to be taken up from daily diets since they cannot be produced by human biological system. Some carotenoids serve as pro-vitamin A that supple human visual and skin health. In this study, we aim to determine the carotenoid(s) content of Ruta angustifolia and their profile in the intact plant, callus and shoot cultures. The callus cultures were induced from stem explants and subsequently subcultured until the calli successfully generated in vitro shoots. The carotenoids extracts from the plant tissues were analysed qualitatively and quantitatively by using UV spectrophotometry and HPLC. The result showed that there was significant difference in accumulation of carotenoid(s) among the plant sources and the most common carotenoids found were β-carotene and lutein. The intact plant showed the highest content of the carotenoids whilst the callus possess the least. The outcome of this study could be used for further manipulation of the in vitro cultures for the carotenoids production enhancement. 2011-11-14 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/8278/1/POSTER.pdf Mohd. Zaifuddin, Fatimah Azzah and Mohd Hassan, Norazian and Othman, Rashidi (2011) Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers. In: International Conference on Natural Products (ICNP 2011): Metabolomics - A New Frontier in Natural Products Science, 14-16 November 2011, Putrajaya, Malaysia. http://www.icnp2011.upm.edu.my
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QK Botany SB Plant culture
spellingShingle	QK Botany SB Plant culture Mohd. Zaifuddin, Fatimah Azzah Mohd Hassan, Norazian Othman, Rashidi Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.
description	Researches concerning antioxidants are extensively studied nowadays. Antioxidants are well known to be associated with reduction in cancer incidence since they act against deteriorative effects of free radicals. These antioxidants include the yellow-to-red coloured pigments known as carotenoids, are essential for betterment in human immunity. Carotenoids can be found abundantly in dark green vegetables and also yellow, orange and red coloured fruits. They have to be taken up from daily diets since they cannot be produced by human biological system. Some carotenoids serve as pro-vitamin A that supple human visual and skin health. In this study, we aim to determine the carotenoid(s) content of Ruta angustifolia and their profile in the intact plant, callus and shoot cultures. The callus cultures were induced from stem explants and subsequently subcultured until the calli successfully generated in vitro shoots. The carotenoids extracts from the plant tissues were analysed qualitatively and quantitatively by using UV spectrophotometry and HPLC. The result showed that there was significant difference in accumulation of carotenoid(s) among the plant sources and the most common carotenoids found were β-carotene and lutein. The intact plant showed the highest content of the carotenoids whilst the callus possess the least. The outcome of this study could be used for further manipulation of the in vitro cultures for the carotenoids production enhancement.
format	Conference or Workshop Item
author	Mohd. Zaifuddin, Fatimah Azzah Mohd Hassan, Norazian Othman, Rashidi
author_facet	Mohd. Zaifuddin, Fatimah Azzah Mohd Hassan, Norazian Othman, Rashidi
author_sort	Mohd. Zaifuddin, Fatimah Azzah
title	Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.
title_short	Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.
title_full	Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.
title_fullStr	Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.
title_full_unstemmed	Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.
title_sort	determination of carotenoid(s) composition in intact plant, callus and shoot cultures of ruta angustifolia (l.) pers.
publishDate	2011
url	http://irep.iium.edu.my/8278/ http://irep.iium.edu.my/8278/ http://irep.iium.edu.my/8278/1/POSTER.pdf
first_indexed	2023-09-18T20:17:57Z
last_indexed	2023-09-18T20:17:57Z
_version_	1777407913881501696

Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.

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