A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana
The sfr3-1 mutation causes freezing-sensitivity in Arabidopsis thaliana. Through mapping, sequencing and transgenic complementation, sfr3-1 was revealed as a missense mutation in ACC1, which is an essential gene encoding multifunctional acetyl-CoA carboxylase. Suppression of ACC1 expression by RNA i...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Asian Network for Scientific Information
2009
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/864/ http://irep.iium.edu.my/864/ http://irep.iium.edu.my/864/ http://irep.iium.edu.my/864/1/144-152.pdf |
| Summary: | The sfr3-1 mutation causes freezing-sensitivity in Arabidopsis thaliana. Through mapping, sequencing and transgenic complementation, sfr3-1 was revealed as a missense mutation in ACC1, which is an essential gene encoding multifunctional acetyl-CoA carboxylase. Suppression of ACC1 expression by RNA interference produced a freezing-sensitive phenotype with some similarity to that of sfr3-1. The dCAPS primers and PCR confirmed that sfr3 gene encodes multifunctional acetyl-CoA carboxylase. Microarray and real-time PCR experiments demonstrated that the expression of ACC1 increase only 1.48 fold in wild-type and 1.35 fold in mutant in response to cold treatment. Studies also suggested that the sfr3-1 mutation is more likely to be a temperature-sensitive mutation as the sfr3 mutant cuticle becomes leaky only at low temperature and this was confirmed by cuticular defects analysis.
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