A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana

The sfr3-1 mutation causes freezing-sensitivity in Arabidopsis thaliana. Through mapping, sequencing and transgenic complementation, sfr3-1 was revealed as a missense mutation in ACC1, which is an essential gene encoding multifunctional acetyl-CoA carboxylase. Suppression of ACC1 expression by RNA i...

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Main Authors:	Amid, Azura, Warren, Garreth J.
Format:	Article
Language:	English
Published:	Asian Network for Scientific Information 2009
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/864/ http://irep.iium.edu.my/864/ http://irep.iium.edu.my/864/ http://irep.iium.edu.my/864/1/144-152.pdf

id	iium-864
recordtype	eprints
spelling	iium-8642011-08-05T07:21:29Z http://irep.iium.edu.my/864/ A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana Amid, Azura Warren, Garreth J. TP248.13 Biotechnology The sfr3-1 mutation causes freezing-sensitivity in Arabidopsis thaliana. Through mapping, sequencing and transgenic complementation, sfr3-1 was revealed as a missense mutation in ACC1, which is an essential gene encoding multifunctional acetyl-CoA carboxylase. Suppression of ACC1 expression by RNA interference produced a freezing-sensitive phenotype with some similarity to that of sfr3-1. The dCAPS primers and PCR confirmed that sfr3 gene encodes multifunctional acetyl-CoA carboxylase. Microarray and real-time PCR experiments demonstrated that the expression of ACC1 increase only 1.48 fold in wild-type and 1.35 fold in mutant in response to cold treatment. Studies also suggested that the sfr3-1 mutation is more likely to be a temperature-sensitive mutation as the sfr3 mutant cuticle becomes leaky only at low temperature and this was confirmed by cuticular defects analysis. Asian Network for Scientific Information 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/864/1/144-152.pdf Amid, Azura and Warren, Garreth J. (2009) A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana. International Journal of Botany, 5 (2). pp. 144-152. ISSN 1811-9719 (O), 1811-9700 (P) http://scialert.net/abstract/?doi=ijb.2009.144.152 DOI: 10.3923/ijb.2009.144.152
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Amid, Azura Warren, Garreth J. A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana
description	The sfr3-1 mutation causes freezing-sensitivity in Arabidopsis thaliana. Through mapping, sequencing and transgenic complementation, sfr3-1 was revealed as a missense mutation in ACC1, which is an essential gene encoding multifunctional acetyl-CoA carboxylase. Suppression of ACC1 expression by RNA interference produced a freezing-sensitive phenotype with some similarity to that of sfr3-1. The dCAPS primers and PCR confirmed that sfr3 gene encodes multifunctional acetyl-CoA carboxylase. Microarray and real-time PCR experiments demonstrated that the expression of ACC1 increase only 1.48 fold in wild-type and 1.35 fold in mutant in response to cold treatment. Studies also suggested that the sfr3-1 mutation is more likely to be a temperature-sensitive mutation as the sfr3 mutant cuticle becomes leaky only at low temperature and this was confirmed by cuticular defects analysis.
format	Article
author	Amid, Azura Warren, Garreth J.
author_facet	Amid, Azura Warren, Garreth J.
author_sort	Amid, Azura
title	A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana
title_short	A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana
title_full	A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana
title_fullStr	A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana
title_full_unstemmed	A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana
title_sort	multifunctional acetyl-coa carboxylase gene confers freesing tolerance in arabidopsis thaliana
publisher	Asian Network for Scientific Information
publishDate	2009
url	http://irep.iium.edu.my/864/ http://irep.iium.edu.my/864/ http://irep.iium.edu.my/864/ http://irep.iium.edu.my/864/1/144-152.pdf
first_indexed	2023-09-18T20:08:03Z
last_indexed	2023-09-18T20:08:03Z
_version_	1777407290988560384

A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana

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