PCR-based chromosome walking: the SFR 3 case

In this study, mapping experiments were initiated in a region of 34.23 cM or 2.7 Mb to map the SFR3 gene into the smallest possible region on chromosome I. Eleven new PCR markers had been designed to fine map the gene. Roughly 557 F2 plants tested in these experiments and 38 lines were then used in...

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Main Authors:	Amid, Azura, Warren, Garreth J., Bramley, Peter M.
Format:	Article
Language:	English
Published:	Asian Network for Scientific Information 2006
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/868/ http://irep.iium.edu.my/868/ http://irep.iium.edu.my/868/1/PCR-based_chromosome_walking.pdf

id	iium-868
recordtype	eprints
spelling	iium-8682011-08-05T07:32:50Z http://irep.iium.edu.my/868/ PCR-based chromosome walking: the SFR 3 case Amid, Azura Warren, Garreth J. Bramley, Peter M. TP248.13 Biotechnology In this study, mapping experiments were initiated in a region of 34.23 cM or 2.7 Mb to map the SFR3 gene into the smallest possible region on chromosome I. Eleven new PCR markers had been designed to fine map the gene. Roughly 557 F2 plants tested in these experiments and 38 lines were then used in the fine mapping experiments. The fine mapping experiments left the SFR3 region amongst 26 genes. Finally, 13 pseudogenes and transposable elements were eliminated and this left the SFR3 gene region amongst 13 genes in a 0.19 Mb region, which is represented by 4 BAC clones. The PCR-based chromosome mapping experiments to fine map the SFR3 gene have successfully decreased the SFR3 region from 2.7 to 0.19 Mb. We concluded that PCR-based chromosome walking is a convenient method to clone SFR3 gene. Asian Network for Scientific Information 2006 Article PeerReviewed application/pdf en http://irep.iium.edu.my/868/1/PCR-based_chromosome_walking.pdf Amid, Azura and Warren, Garreth J. and Bramley, Peter M. (2006) PCR-based chromosome walking: the SFR 3 case. Pakistan Journal of Biological Sciences, 9 (1). pp. 164-169. ISSN 1812-5735 (O), 1028-8880 (P) http://docsdrive.com/pdfs/ansinet/pjbs/2006/164-169.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Amid, Azura Warren, Garreth J. Bramley, Peter M. PCR-based chromosome walking: the SFR 3 case
description	In this study, mapping experiments were initiated in a region of 34.23 cM or 2.7 Mb to map the SFR3 gene into the smallest possible region on chromosome I. Eleven new PCR markers had been designed to fine map the gene. Roughly 557 F2 plants tested in these experiments and 38 lines were then used in the fine mapping experiments. The fine mapping experiments left the SFR3 region amongst 26 genes. Finally, 13 pseudogenes and transposable elements were eliminated and this left the SFR3 gene region amongst 13 genes in a 0.19 Mb region, which is represented by 4 BAC clones. The PCR-based chromosome mapping experiments to fine map the SFR3 gene have successfully decreased the SFR3 region from 2.7 to 0.19 Mb. We concluded that PCR-based chromosome walking is a convenient method to clone SFR3 gene.
format	Article
author	Amid, Azura Warren, Garreth J. Bramley, Peter M.
author_facet	Amid, Azura Warren, Garreth J. Bramley, Peter M.
author_sort	Amid, Azura
title	PCR-based chromosome walking: the SFR 3 case
title_short	PCR-based chromosome walking: the SFR 3 case
title_full	PCR-based chromosome walking: the SFR 3 case
title_fullStr	PCR-based chromosome walking: the SFR 3 case
title_full_unstemmed	PCR-based chromosome walking: the SFR 3 case
title_sort	pcr-based chromosome walking: the sfr 3 case
publisher	Asian Network for Scientific Information
publishDate	2006
url	http://irep.iium.edu.my/868/ http://irep.iium.edu.my/868/ http://irep.iium.edu.my/868/1/PCR-based_chromosome_walking.pdf
first_indexed	2023-09-18T20:08:03Z
last_indexed	2023-09-18T20:08:03Z
_version_	1777407291422670848

PCR-based chromosome walking: the SFR 3 case

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