Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion

A study was undertaken to observe the effect of D-glucose and L-glutamine and the combination of both nutrients at various concentrations towards the growth and insulin production by BRIN-BD11 cells. It was found that at a final concentration of 4 g/L D-glucose and 0.3 g/L L-glutamine in RPMI-1640 m...

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Bibliographic Details
Main Author:	Abdul Karim, Mohamed Ismail
Format:	Conference or Workshop Item
Language:	English
Published:	2009
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/8909/ http://irep.iium.edu.my/8909/1/Seminar_on_Research_Findings_Series_No_2__2009__-_Prof_Ismail.pdf

id	iium-8909
recordtype	eprints
spelling	iium-89092012-04-04T06:54:39Z http://irep.iium.edu.my/8909/ Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion Abdul Karim, Mohamed Ismail TP248.13 Biotechnology A study was undertaken to observe the effect of D-glucose and L-glutamine and the combination of both nutrients at various concentrations towards the growth and insulin production by BRIN-BD11 cells. It was found that at a final concentration of 4 g/L D-glucose and 0.3 g/L L-glutamine in RPMI-1640 media gave maximum cell growth and insulin production of 1.75 x 106 cells/ml from 2.5 x 105 cells/ml and 100.77 µg/L, respectively. The result of this study indicates that D-glucose and L-glutamine promote good cell growth and insulin secretion of BRIN-BD11 cells as a step towards mass propagation of transplantable insulinoma cells and insulin production. 2009 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/8909/1/Seminar_on_Research_Findings_Series_No_2__2009__-_Prof_Ismail.pdf Abdul Karim, Mohamed Ismail (2009) Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion. In: Seminar on Research Findings Series No. 2, Kuala Lumpur, Malaysia. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Abdul Karim, Mohamed Ismail Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion
description	A study was undertaken to observe the effect of D-glucose and L-glutamine and the combination of both nutrients at various concentrations towards the growth and insulin production by BRIN-BD11 cells. It was found that at a final concentration of 4 g/L D-glucose and 0.3 g/L L-glutamine in RPMI-1640 media gave maximum cell growth and insulin production of 1.75 x 106 cells/ml from 2.5 x 105 cells/ml and 100.77 µg/L, respectively. The result of this study indicates that D-glucose and L-glutamine promote good cell growth and insulin secretion of BRIN-BD11 cells as a step towards mass propagation of transplantable insulinoma cells and insulin production.
format	Conference or Workshop Item
author	Abdul Karim, Mohamed Ismail
author_facet	Abdul Karim, Mohamed Ismail
author_sort	Abdul Karim, Mohamed Ismail
title	Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion
title_short	Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion
title_full	Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion
title_fullStr	Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion
title_full_unstemmed	Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion
title_sort	optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion
publishDate	2009
url	http://irep.iium.edu.my/8909/ http://irep.iium.edu.my/8909/1/Seminar_on_Research_Findings_Series_No_2__2009__-_Prof_Ismail.pdf
first_indexed	2023-09-18T20:18:43Z
last_indexed	2023-09-18T20:18:43Z
_version_	1777407962099220480

Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion

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