In vitro antioxidant and antiproliferative activities of three Malaysian sea cucumber species

The antioxidant and antiproliferative activities of aqueous and organic extracts from three sea cucumber species Holothuria scabra, Holothuria leucospilota and Stichopus chloronotus were studied. The result showed that aqueous extract of H. leucospilota contains the highest level of total phenols (9...

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Main Authors:	Althunibat, Osama Yousef, Hashim, Ridzwan, Bakhtiar, M. Taher, Mohd. Daud, Jamaludin, Ikeda, Masa-Aki, Ibrahim, Zali
Format:	Article
Language:	English
Published:	EuroJournals Publishing, Inc. 2009
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/903/ http://irep.iium.edu.my/903/ http://irep.iium.edu.my/903/1/In_Vitro_Antioxidant_and_Antiproliferative_Activities_of_Three.pdf

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recordtype	eprints
spelling	iium-9032017-06-20T07:47:50Z http://irep.iium.edu.my/903/ In vitro antioxidant and antiproliferative activities of three Malaysian sea cucumber species Althunibat, Osama Yousef Hashim, Ridzwan Bakhtiar, M. Taher Mohd. Daud, Jamaludin Ikeda, Masa-Aki Ibrahim, Zali RB Pathology The antioxidant and antiproliferative activities of aqueous and organic extracts from three sea cucumber species Holothuria scabra, Holothuria leucospilota and Stichopus chloronotus were studied. The result showed that aqueous extract of H. leucospilota contains the highest level of total phenols (9.7 mg GAE/g), while the lowest level was obtained from organic extract of H. scabra (1.53 mg GAE/g). An aqueous extract of S. chloronotus was the most efficient extract in scavenging of DPPH free radical, giving IC50 = 2.13 mg/ml. The aqueous extracts (50mg/ml) of H. scabra, H. leucospilota and S. chloronotus exhibited high antioxidant activities (77.46%, 64.03% and 80.58%, respectively) against linoleic acid free radical. Only an aqueous extract of S. chloronotus inhibited growth of C33A (human cervical cancer) and A549 (human non-small lung carcinoma) cancer cells, giving IC50 equal to 10.0 µg/ml and 28.0 µg/ml, respectively. On the other hand, the dose dependent antiproliferative effects of all organic extracts studied were observed on C33A and A549 cancer cells. This study, therefore, revealed that all the three species of Malaysian sea cucumber are potential sources of natural antioxidant and anticancer agents. EuroJournals Publishing, Inc. 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/903/1/In_Vitro_Antioxidant_and_Antiproliferative_Activities_of_Three.pdf Althunibat, Osama Yousef and Hashim, Ridzwan and Bakhtiar, M. Taher and Mohd. Daud, Jamaludin and Ikeda, Masa-Aki and Ibrahim, Zali (2009) In vitro antioxidant and antiproliferative activities of three Malaysian sea cucumber species. European Journal of Scientific Research, 37 (3). pp. 376-387. ISSN 1450-216X http://www.britannica.com/bps/additionalcontent/18/50881911/In-Vitro-Antioxidant-and-Antiproliferative-Activities-of-Three-Malaysian-Sea-Cucumber-Species
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RB Pathology
spellingShingle	RB Pathology Althunibat, Osama Yousef Hashim, Ridzwan Bakhtiar, M. Taher Mohd. Daud, Jamaludin Ikeda, Masa-Aki Ibrahim, Zali In vitro antioxidant and antiproliferative activities of three Malaysian sea cucumber species
description	The antioxidant and antiproliferative activities of aqueous and organic extracts from three sea cucumber species Holothuria scabra, Holothuria leucospilota and Stichopus chloronotus were studied. The result showed that aqueous extract of H. leucospilota contains the highest level of total phenols (9.7 mg GAE/g), while the lowest level was obtained from organic extract of H. scabra (1.53 mg GAE/g). An aqueous extract of S. chloronotus was the most efficient extract in scavenging of DPPH free radical, giving IC50 = 2.13 mg/ml. The aqueous extracts (50mg/ml) of H. scabra, H. leucospilota and S. chloronotus exhibited high antioxidant activities (77.46%, 64.03% and 80.58%, respectively) against linoleic acid free radical. Only an aqueous extract of S. chloronotus inhibited growth of C33A (human cervical cancer) and A549 (human non-small lung carcinoma) cancer cells, giving IC50 equal to 10.0 µg/ml and 28.0 µg/ml, respectively. On the other hand, the dose dependent antiproliferative effects of all organic extracts studied were observed on C33A and A549 cancer cells. This study, therefore, revealed that all the three species of Malaysian sea cucumber are potential sources of natural antioxidant and anticancer agents.
format	Article
author	Althunibat, Osama Yousef Hashim, Ridzwan Bakhtiar, M. Taher Mohd. Daud, Jamaludin Ikeda, Masa-Aki Ibrahim, Zali
author_facet	Althunibat, Osama Yousef Hashim, Ridzwan Bakhtiar, M. Taher Mohd. Daud, Jamaludin Ikeda, Masa-Aki Ibrahim, Zali
author_sort	Althunibat, Osama Yousef
title	In vitro antioxidant and antiproliferative activities of three Malaysian sea cucumber species
title_short	In vitro antioxidant and antiproliferative activities of three Malaysian sea cucumber species
title_full	In vitro antioxidant and antiproliferative activities of three Malaysian sea cucumber species
title_fullStr	In vitro antioxidant and antiproliferative activities of three Malaysian sea cucumber species
title_full_unstemmed	In vitro antioxidant and antiproliferative activities of three Malaysian sea cucumber species
title_sort	in vitro antioxidant and antiproliferative activities of three malaysian sea cucumber species
publisher	EuroJournals Publishing, Inc.
publishDate	2009
url	http://irep.iium.edu.my/903/ http://irep.iium.edu.my/903/ http://irep.iium.edu.my/903/1/In_Vitro_Antioxidant_and_Antiproliferative_Activities_of_Three.pdf
first_indexed	2023-09-18T20:08:05Z
last_indexed	2023-09-18T20:08:05Z
_version_	1777407293614194688

In vitro antioxidant and antiproliferative activities of three Malaysian sea cucumber species

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