Optimization of CNTs production using full factorial design and its advanced application in protein purification

Carbon nanotubes (CNTs) have been successfully synthesized by using in-house fabricated Double Stage Chemical Vapor Deposition (DS-CVD) technique, using acetylene (C2H2) and hydrogen (H2) as the precursor gases. The purity, morphology and the structure of CNTs were then characterized using Field Emi...

Full description

Bibliographic Details
Main Authors:	Mubarak, N. M., Yusof, Faridah, Al-Khatib, Ma An Fahmi Rashid, Ameen, Emad S.M, Khalid, M., Al-Sa'adi, Abdul Razak Abdul Rahman Asaad, Atieh, Muataz A., Qudsieh, Isam Y., Walvekar , Rashmi Gangasa
Format:	Article
Language:	English
Published:	World Scientific Publishing Co. 2010
Subjects:	TP155 Chemical engineering
Online Access:	http://irep.iium.edu.my/9230/ http://irep.iium.edu.my/9230/ http://irep.iium.edu.my/9230/ http://irep.iium.edu.my/9230/1/IJN_final_paper.pdf

id	iium-9230
recordtype	eprints
spelling	iium-92302012-07-06T07:09:07Z http://irep.iium.edu.my/9230/ Optimization of CNTs production using full factorial design and its advanced application in protein purification Mubarak, N. M. Yusof, Faridah Al-Khatib, Ma An Fahmi Rashid Ameen, Emad S.M Khalid, M. Al-Sa'adi, Abdul Razak Abdul Rahman Asaad Atieh, Muataz A. Qudsieh, Isam Y. Walvekar , Rashmi Gangasa TP155 Chemical engineering Carbon nanotubes (CNTs) have been successfully synthesized by using in-house fabricated Double Stage Chemical Vapor Deposition (DS-CVD) technique, using acetylene (C2H2) and hydrogen (H2) as the precursor gases. The purity, morphology and the structure of CNTs were then characterized using Field Emission Scanning Microscope (FESEM), Transmission Electron Microscope (TEM) and Thermogravimetric Analysis (TGA). The effects of the process parameters were examined whereby the experimental design of the investigation was conducted using Design Expert® Version 6.0.8. The statistical analysis reveals that the optimized conditions for the best CNTs yield production at 850°C reaction temperature, 60 min reaction time, with gas flow rates at 40 and 150 ml/min for C2H2 and H2, respectively. The CNTs produced were successfully used as column chromatographic media. Due to its nanosized structured dimension, CNTs' have tremendously large surface area and that lead to highly efficient protein purification. Skim latex protein has been used as the model protein and we aim to recover useful proteins and enzymes from this known wasteful material. During the purification, the process parameters such as pH and ionic strength of the running buffer were optimized to enhance protein purification. Results reveal that CNTs behave efficiently as a hydrophobic interaction chromatographic media. World Scientific Publishing Co. 2010-10-20 Article PeerReviewed application/pdf en http://irep.iium.edu.my/9230/1/IJN_final_paper.pdf Mubarak, N. M. and Yusof, Faridah and Al-Khatib, Ma An Fahmi Rashid and Ameen, Emad S.M and Khalid, M. and Al-Sa'adi, Abdul Razak Abdul Rahman Asaad and Atieh, Muataz A. and Qudsieh, Isam Y. and Walvekar , Rashmi Gangasa (2010) Optimization of CNTs production using full factorial design and its advanced application in protein purification. International Journal of Nanoscience, 9 (3). pp. 181-192. ISSN 0219-581X (P), 1793-5350 (O) http://www.worldscinet.com/ijn/09/0903/S0219581X10006648.html 10.1142/S0219581X10006648
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering
spellingShingle	TP155 Chemical engineering Mubarak, N. M. Yusof, Faridah Al-Khatib, Ma An Fahmi Rashid Ameen, Emad S.M Khalid, M. Al-Sa'adi, Abdul Razak Abdul Rahman Asaad Atieh, Muataz A. Qudsieh, Isam Y. Walvekar , Rashmi Gangasa Optimization of CNTs production using full factorial design and its advanced application in protein purification
description	Carbon nanotubes (CNTs) have been successfully synthesized by using in-house fabricated Double Stage Chemical Vapor Deposition (DS-CVD) technique, using acetylene (C2H2) and hydrogen (H2) as the precursor gases. The purity, morphology and the structure of CNTs were then characterized using Field Emission Scanning Microscope (FESEM), Transmission Electron Microscope (TEM) and Thermogravimetric Analysis (TGA). The effects of the process parameters were examined whereby the experimental design of the investigation was conducted using Design Expert® Version 6.0.8. The statistical analysis reveals that the optimized conditions for the best CNTs yield production at 850°C reaction temperature, 60 min reaction time, with gas flow rates at 40 and 150 ml/min for C2H2 and H2, respectively. The CNTs produced were successfully used as column chromatographic media. Due to its nanosized structured dimension, CNTs' have tremendously large surface area and that lead to highly efficient protein purification. Skim latex protein has been used as the model protein and we aim to recover useful proteins and enzymes from this known wasteful material. During the purification, the process parameters such as pH and ionic strength of the running buffer were optimized to enhance protein purification. Results reveal that CNTs behave efficiently as a hydrophobic interaction chromatographic media.
format	Article
author	Mubarak, N. M. Yusof, Faridah Al-Khatib, Ma An Fahmi Rashid Ameen, Emad S.M Khalid, M. Al-Sa'adi, Abdul Razak Abdul Rahman Asaad Atieh, Muataz A. Qudsieh, Isam Y. Walvekar , Rashmi Gangasa
author_facet	Mubarak, N. M. Yusof, Faridah Al-Khatib, Ma An Fahmi Rashid Ameen, Emad S.M Khalid, M. Al-Sa'adi, Abdul Razak Abdul Rahman Asaad Atieh, Muataz A. Qudsieh, Isam Y. Walvekar , Rashmi Gangasa
author_sort	Mubarak, N. M.
title	Optimization of CNTs production using full factorial design and its advanced application in protein purification
title_short	Optimization of CNTs production using full factorial design and its advanced application in protein purification
title_full	Optimization of CNTs production using full factorial design and its advanced application in protein purification
title_fullStr	Optimization of CNTs production using full factorial design and its advanced application in protein purification
title_full_unstemmed	Optimization of CNTs production using full factorial design and its advanced application in protein purification
title_sort	optimization of cnts production using full factorial design and its advanced application in protein purification
publisher	World Scientific Publishing Co.
publishDate	2010
url	http://irep.iium.edu.my/9230/ http://irep.iium.edu.my/9230/ http://irep.iium.edu.my/9230/ http://irep.iium.edu.my/9230/1/IJN_final_paper.pdf
first_indexed	2023-09-18T20:18:58Z
last_indexed	2023-09-18T20:18:58Z
_version_	1777407978420305920

Optimization of CNTs production using full factorial design and its advanced application in protein purification

Similar Items