Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate

In the present study, optimizing critical nutritional constituents was attempted as a primary strategy to improve biosurfactant production from Klebsiella pneumoniae WMF02 in liquid state fermentation utilizing sludge palm oil as a substrate. One-factor-at-a-time (OFAT) optimization was employed to...

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Main Authors:	Jamal, Parveen, Wan Nawawi, Wan Mohd Fazli, Alam, Md. Zahangir
Format:	Article
Language:	English
Published:	INSI Publications 2012
Subjects:	TA164 Bioengineering TA170 Environmental engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/9757/ http://irep.iium.edu.my/9757/ http://irep.iium.edu.my/9757/1/Optimum_Medium_Components_for_Biosurfactant_Production.pdf

id	iium-9757
recordtype	eprints
spelling	iium-97572017-08-10T01:50:02Z http://irep.iium.edu.my/9757/ Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate Jamal, Parveen Wan Nawawi, Wan Mohd Fazli Alam, Md. Zahangir TA164 Bioengineering TA170 Environmental engineering TP248.13 Biotechnology In the present study, optimizing critical nutritional constituents was attempted as a primary strategy to improve biosurfactant production from Klebsiella pneumoniae WMF02 in liquid state fermentation utilizing sludge palm oil as a substrate. One-factor-at-a-time (OFAT) optimization was employed to evaluate the effects of sludge palm oil (SPO), sucrose, MgSO4, FeSO4, NaNO3 and K2HPO4 on surface tension reductivity. Sucrose was selected as a co-substrate over glucose in production media. The optimal levels of the aforementioned variables were (g/l) sucrose 5.0, MgSO4 0.4, FeSO4 0.3, NaNO3 2.0, and K2HPO4 4.0, with SPO concentration of 4% (v/v). The optimized medium shows surface tension reduction from 36.2 mN/m (non-optimized medium) to 25.70 mN/m. Preliminary biosurfactant identification indicated that the biosurfactant produced was phospholipids in nature. INSI Publications 2012-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/9757/1/Optimum_Medium_Components_for_Biosurfactant_Production.pdf Jamal, Parveen and Wan Nawawi, Wan Mohd Fazli and Alam, Md. Zahangir (2012) Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate. Australian Journal of Basic and Applied Sciences, 6 (1). pp. 100-108. ISSN 1991-8178 http://www.insipub.com/ajbas/2012/January/100-108.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TA164 Bioengineering TA170 Environmental engineering TP248.13 Biotechnology
spellingShingle	TA164 Bioengineering TA170 Environmental engineering TP248.13 Biotechnology Jamal, Parveen Wan Nawawi, Wan Mohd Fazli Alam, Md. Zahangir Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate
description	In the present study, optimizing critical nutritional constituents was attempted as a primary strategy to improve biosurfactant production from Klebsiella pneumoniae WMF02 in liquid state fermentation utilizing sludge palm oil as a substrate. One-factor-at-a-time (OFAT) optimization was employed to evaluate the effects of sludge palm oil (SPO), sucrose, MgSO4, FeSO4, NaNO3 and K2HPO4 on surface tension reductivity. Sucrose was selected as a co-substrate over glucose in production media. The optimal levels of the aforementioned variables were (g/l) sucrose 5.0, MgSO4 0.4, FeSO4 0.3, NaNO3 2.0, and K2HPO4 4.0, with SPO concentration of 4% (v/v). The optimized medium shows surface tension reduction from 36.2 mN/m (non-optimized medium) to 25.70 mN/m. Preliminary biosurfactant identification indicated that the biosurfactant produced was phospholipids in nature.
format	Article
author	Jamal, Parveen Wan Nawawi, Wan Mohd Fazli Alam, Md. Zahangir
author_facet	Jamal, Parveen Wan Nawawi, Wan Mohd Fazli Alam, Md. Zahangir
author_sort	Jamal, Parveen
title	Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate
title_short	Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate
title_full	Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate
title_fullStr	Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate
title_full_unstemmed	Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate
title_sort	optimum medium components for biosurfactant production by klebsiella pneumoniae wmf02 utilizing sludge palm oil as a substrate
publisher	INSI Publications
publishDate	2012
url	http://irep.iium.edu.my/9757/ http://irep.iium.edu.my/9757/ http://irep.iium.edu.my/9757/1/Optimum_Medium_Components_for_Biosurfactant_Production.pdf
first_indexed	2023-09-18T20:19:22Z
last_indexed	2023-09-18T20:19:22Z
_version_	1777408003149922304

Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate

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