Cloning, expression, purification, crystallization and preliminary X-ray studies of the C-terminal domain of Rv3262 (FbiB) from Mycobacterium tuberculosis

During cofactor F420 biosynthesis, the enzyme F420-[gamma]-glutamyl ligase (FbiB) catalyzes the addition of [gamma]-linked L-glutamate residues to form polyglutamylated F420 derivatives. In Mycobacterium tuberculosis, Rv3262 (FbiB) consists of two domains: an N-terminal domain from the F420 ligase s...

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Bibliographic Details
Main Authors: Mohamed Rehan, Aisyah, Paterson, Neil G., Baker, Edward N., Squire, Christopher J.
Format: Article
Language:English
Published: Wiley-Blackwell Publishing, Inc. 2011
Subjects:
Online Access:http://irep.iium.edu.my/9893/
http://irep.iium.edu.my/9893/
http://irep.iium.edu.my/9893/
http://irep.iium.edu.my/9893/4/CLONING%2CEXPRESSION%2C_PURIFICATION%2C_CRYSTALLIZATION_AND_PRELIMINARY_X-RAY_STUDIES_OF_THE_C-TERMINAL.pdf

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