Phylogenetic relationship of non-typeable haemophilus influenzaeisolates / Nurul Hamirah Kamsani

Nontypeable Haemophilus influenzae (NTHi) is a significant pathogen in children, causing otitis media, sinusitis, conjunctivitis, pneumonia, and occasionally invasive infections. Twenty-eight strains obtained from the Institute for Medical Research (IMR), Kuala Lumpur, were examined for antimicro...

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Main Author:	Kamsani, Nurul Hamirah
Format:	Thesis
Language:	English
Published:	2014
Subjects:	QR Microbiology Bacteria
Online Access:	http://ir.uitm.edu.my/id/eprint/14289/ http://ir.uitm.edu.my/id/eprint/14289/1/TM_NURUL%20HAMIRAH%20KAMSANI%20MD%2014_5.pdf

id	uitm-14289
recordtype	eprints
spelling	uitm-142892016-08-24T06:37:34Z http://ir.uitm.edu.my/id/eprint/14289/ Phylogenetic relationship of non-typeable haemophilus influenzaeisolates / Nurul Hamirah Kamsani Kamsani, Nurul Hamirah QR Microbiology Bacteria Nontypeable Haemophilus influenzae (NTHi) is a significant pathogen in children, causing otitis media, sinusitis, conjunctivitis, pneumonia, and occasionally invasive infections. Twenty-eight strains obtained from the Institute for Medical Research (IMR), Kuala Lumpur, were examined for antimicrobial susceptibility pattern and the relationship between the strains was determined by using three different molecular epidemiological methods; restriction fragment length polymorphism (RFLP), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Antibiotic susceptibility test showed 33% of the strains were resistant to two or more of the tested antimicrobial agents. These NTHi strains demonstrated resistance most frequently to trimethoprim-sulfomethoxazole (32%), followed by ampicilin (21%), erythromycin (10%) and 7% each for chloramphenicol and streptomycin. Digestion with HinlIII for RFLP demonstrated limited use because it produced only two to three bands for each strains. When analysed by PFGE, a total of 25 PFGE patterns were produced with SmaI, representing a genetically assorted population. It was observed that the strains fell into four major clusters with genetic distances >40% similarity of which only six strains were clonal with more than 900/0 similarity. While for MLST typing, new combination of allele numbers were found and 27 novel sequence types (STs) were detected. Among the three methods, RFLP was less discriminating in comparison to the PFGE and MLST. PFGE provides information on the relatedness between strains while the MLST reveals the genetic variation amongst Malaysian NTHi strains and their relationship to all the H influenzae isolates in the MLST database. Based on all the three typing methods, this study shows that majority of the NTHi strains in Malaysia are heterogenous and are genetically diversified. 2014 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/14289/1/TM_NURUL%20HAMIRAH%20KAMSANI%20MD%2014_5.pdf Kamsani, Nurul Hamirah (2014) Phylogenetic relationship of non-typeable haemophilus influenzaeisolates / Nurul Hamirah Kamsani. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	QR Microbiology Bacteria
spellingShingle	QR Microbiology Bacteria Kamsani, Nurul Hamirah Phylogenetic relationship of non-typeable haemophilus influenzaeisolates / Nurul Hamirah Kamsani
description	Nontypeable Haemophilus influenzae (NTHi) is a significant pathogen in children, causing otitis media, sinusitis, conjunctivitis, pneumonia, and occasionally invasive infections. Twenty-eight strains obtained from the Institute for Medical Research (IMR), Kuala Lumpur, were examined for antimicrobial susceptibility pattern and the relationship between the strains was determined by using three different molecular epidemiological methods; restriction fragment length polymorphism (RFLP), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Antibiotic susceptibility test showed 33% of the strains were resistant to two or more of the tested antimicrobial agents. These NTHi strains demonstrated resistance most frequently to trimethoprim-sulfomethoxazole (32%), followed by ampicilin (21%), erythromycin (10%) and 7% each for chloramphenicol and streptomycin. Digestion with HinlIII for RFLP demonstrated limited use because it produced only two to three bands for each strains. When analysed by PFGE, a total of 25 PFGE patterns were produced with SmaI, representing a genetically assorted population. It was observed that the strains fell into four major clusters with genetic distances >40% similarity of which only six strains were clonal with more than 900/0 similarity. While for MLST typing, new combination of allele numbers were found and 27 novel sequence types (STs) were detected. Among the three methods, RFLP was less discriminating in comparison to the PFGE and MLST. PFGE provides information on the relatedness between strains while the MLST reveals the genetic variation amongst Malaysian NTHi strains and their relationship to all the H influenzae isolates in the MLST database. Based on all the three typing methods, this study shows that majority of the NTHi strains in Malaysia are heterogenous and are genetically diversified.
format	Thesis
author	Kamsani, Nurul Hamirah
author_facet	Kamsani, Nurul Hamirah
author_sort	Kamsani, Nurul Hamirah
title	Phylogenetic relationship of non-typeable haemophilus influenzaeisolates / Nurul Hamirah Kamsani
title_short	Phylogenetic relationship of non-typeable haemophilus influenzaeisolates / Nurul Hamirah Kamsani
title_full	Phylogenetic relationship of non-typeable haemophilus influenzaeisolates / Nurul Hamirah Kamsani
title_fullStr	Phylogenetic relationship of non-typeable haemophilus influenzaeisolates / Nurul Hamirah Kamsani
title_full_unstemmed	Phylogenetic relationship of non-typeable haemophilus influenzaeisolates / Nurul Hamirah Kamsani
title_sort	phylogenetic relationship of non-typeable haemophilus influenzaeisolates / nurul hamirah kamsani
publishDate	2014
url	http://ir.uitm.edu.my/id/eprint/14289/ http://ir.uitm.edu.my/id/eprint/14289/1/TM_NURUL%20HAMIRAH%20KAMSANI%20MD%2014_5.pdf
first_indexed	2023-09-18T22:51:17Z
last_indexed	2023-09-18T22:51:17Z
_version_	1777417561398312960

Phylogenetic relationship of non-typeable haemophilus influenzaeisolates / Nurul Hamirah Kamsani

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