Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah

Rapid increase in the industrial application of nanomaterials such as MgO nanoparticles has great impact on the public. MgO nanomaterial is now used in medical and pharmaceutical products as well as industrial products such as paints.Thus, they are now widely used by the public, but their significan...

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Main Author:	Hasbullah, Nur Intan
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Physical and theoretical chemistry Analytical chemistry
Online Access:	http://ir.uitm.edu.my/id/eprint/15416/ http://ir.uitm.edu.my/id/eprint/15416/1/TM_NUR%20INTAN%20HASBULLAH%20AS%2015_5.pdf

id	uitm-15416
recordtype	eprints
spelling	uitm-154162016-11-23T06:33:56Z http://ir.uitm.edu.my/id/eprint/15416/ Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah Hasbullah, Nur Intan Physical and theoretical chemistry Analytical chemistry Rapid increase in the industrial application of nanomaterials such as MgO nanoparticles has great impact on the public. MgO nanomaterial is now used in medical and pharmaceutical products as well as industrial products such as paints.Thus, they are now widely used by the public, but their significant potential risks of exposure have not been systematically investigated. In this study, toxic effects of MgO nanoparticles which differ in terms of size and physical dimensions were investigated against cancer neuroblastoma SH-SY5Y cell lines and neuron-phenotypic cell lines. Viability of cells was measured by using MTS assay. Three types of MgO were used in the study, of which two of them were ultra-thin MgO sheets designated as MgO-5 (size: 500 \|im, 5 nm thickness) and MgO-10 (size: 500 \|im, 2 nm thickness). The other nanoparticle was spherical with average size of 10-100 nm (MgO-24). Oxidative stress was measured by the production of reactive oxygen species (ROS) using dicholofluorescein (DCF) assay. Beneficial effects of MgO nanoparticles were also evaluated for potential neuroprotection. Results indicated that the toxic effects of MgO nanoparticles on cancer neuroblastoma SH-SY5Y cell lines and neuron-phenotypic cell lines were dependent on shape, concentration and size of MgO nanoparticles. Particles with spherical shape (MgO-24) exerted more toxic effects compared to ultra-thin nanosheet particles (MgO-5 and MgO-10). MgO nanoparticles were not toxic to both cell lines at concentration <100 \|ng/ml and did not exert any potential for neuroprotection. However, once the concentration was increased to 1 mg/ml, MgO nanoparticles significantly reduced the viability of both cell lines tested due the production of ROS. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/15416/1/TM_NUR%20INTAN%20HASBULLAH%20AS%2015_5.pdf Hasbullah, Nur Intan (2015) Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Physical and theoretical chemistry Analytical chemistry
spellingShingle	Physical and theoretical chemistry Analytical chemistry Hasbullah, Nur Intan Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah
description	Rapid increase in the industrial application of nanomaterials such as MgO nanoparticles has great impact on the public. MgO nanomaterial is now used in medical and pharmaceutical products as well as industrial products such as paints.Thus, they are now widely used by the public, but their significant potential risks of exposure have not been systematically investigated. In this study, toxic effects of MgO nanoparticles which differ in terms of size and physical dimensions were investigated against cancer neuroblastoma SH-SY5Y cell lines and neuron-phenotypic cell lines. Viability of cells was measured by using MTS assay. Three types of MgO were used in the study, of which two of them were ultra-thin MgO sheets designated as MgO-5 (size: 500 \|im, 5 nm thickness) and MgO-10 (size: 500 \|im, 2 nm thickness). The other nanoparticle was spherical with average size of 10-100 nm (MgO-24). Oxidative stress was measured by the production of reactive oxygen species (ROS) using dicholofluorescein (DCF) assay. Beneficial effects of MgO nanoparticles were also evaluated for potential neuroprotection. Results indicated that the toxic effects of MgO nanoparticles on cancer neuroblastoma SH-SY5Y cell lines and neuron-phenotypic cell lines were dependent on shape, concentration and size of MgO nanoparticles. Particles with spherical shape (MgO-24) exerted more toxic effects compared to ultra-thin nanosheet particles (MgO-5 and MgO-10). MgO nanoparticles were not toxic to both cell lines at concentration <100 \|ng/ml and did not exert any potential for neuroprotection. However, once the concentration was increased to 1 mg/ml, MgO nanoparticles significantly reduced the viability of both cell lines tested due the production of ROS.
format	Thesis
author	Hasbullah, Nur Intan
author_facet	Hasbullah, Nur Intan
author_sort	Hasbullah, Nur Intan
title	Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah
title_short	Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah
title_full	Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah
title_fullStr	Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah
title_full_unstemmed	Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah
title_sort	toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / nur intan hasbullah
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/15416/ http://ir.uitm.edu.my/id/eprint/15416/1/TM_NUR%20INTAN%20HASBULLAH%20AS%2015_5.pdf
first_indexed	2023-09-18T22:53:48Z
last_indexed	2023-09-18T22:53:48Z
_version_	1777417719037034496

Toxic effects of magnesium oxide nanoparticles on human neuronal cell lines / Nur Intan Hasbullah

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